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Citations for Miltenyi Biotec :
1 - 50 of 709 citations for Cytomegalovirus Glycoprotein B gB Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Two positive controls for activation were used: PepTivator against cytomegalovirus (Pep-CMV, Miltenyi Biotec), which consisted of 15-mer peptides with 11 amino acids ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with mouse Fc-blocking reagent (Miltenyi Biotec) plus Viakrom-808 (Bekman Culter ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... B cells were isolated from total splenocytes using naïve mouse B-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were immunomagnetically separated using the MACS mouse B cells isolation kit (Miltenyi Biotec, Auburn, CA) and L.S ...
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bioRxiv - Microbiology 2020Quote: ... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ...
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bioRxiv - Microbiology 2020Quote: ... and Fc-receptors were blocked by the mouse FcR Blocking Reagent (Miltenyi Biotec)(0.5μL/well ...
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bioRxiv - Physiology 2023Quote: ... Cells were blocked with mouse Fc receptor block (Miltenyi Biotec, San Diego, CA) and stained with conjugated primary antibodies ...
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bioRxiv - Immunology 2019Quote: ... Murine B cells were enriched by negative selection of IgMpos cells with a mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Immunology 2020Quote: B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... before cells were incubated in anti-mouse IgG 2a+b microbeads (Miltenyi Biotec) for 20 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and the membrane glycoprotein (PepTivator® SARS-CoV-2 Prot_M) were obtained from Miltenyi Biotec. The PepTivator® Peptide Pools are constituted by peptides of 15 amino acid length with 11 amino acid overlap ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Molecular Biology 2021Quote: Nave B lymphocytes were purified from mouse spleens by negative selection using CD43 (Ly48) magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions. ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor block (Miltenyi Biotec Inc) was added and incubated on ice-water bath for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Fc receptor blocker (Miltenyi Biotec Inc) was added and ice-water bath was used for 10 minutes ...
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bioRxiv - Immunology 2019Quote: ... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
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bioRxiv - Immunology 2024Quote: ... containing Fc receptor blocker (Miltenyi Biotec) and incubated for 10 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2022Quote: Naïve B cells (CD43-B220+CD3-) were obtained by negative selection (mouse CD43 Ly-48 MicroBeads; MACS Miltenyi Biotec) from spleens of WT and Lrba-/- mice with 95% purity ...
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched with the B cell isolation Kit II (Miltenyi biotec) and stained with a recombinant ...
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Microbiology 2020Quote: ... human Fc blocking reagent (MACS Miltenyi Biotec) was added to this blocking step ...
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bioRxiv - Cancer Biology 2022Quote: ... the Fc receptor was blocked (Miltenyi Biotec) and cells were stained with the following antibodies for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were negatively sorted using the Pan B cell isolation kit (Miltenyi Biotec). Induction of germinal center B-cell differentiation was done following the protocol of T ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched using a B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) from spleens of immunized mice ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were isolated from PBMCs by using CD19+ B cell isolation kits (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Immunology 2023Quote: ... and total splenic B cells were purified using a negative B cell isolation kit (Miltenyi Biotec). Purity of enriched B cells was determined by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...