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Citations for Miltenyi Biotec :
1 - 50 of 1187 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Immunology 2022Quote: ... with the 37°C Multi-B program of a gentleMACS Dissociator (Miltenyi Biotec). The resulting single cell suspension was passed through a 70 µm cell strainer (Falcon) ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Neuroscience 2021Quote: ... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used were: CD19 PE (LT19) and CD138 APC (B-B4; Miltenyi Biotec); CD23 APC (M-L233) ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Cancer Biology 2021Quote: ... After incubation each sample was placed in 9ml ice-cold 0.04% BSA/PBS in a C-type GentleMacs tube (Miltenyi Biotec). Tissue was further dissociated using a GentleMACs dissociator with programs A1 and two rounds of program B1 with the sample kept on ice between programs ...
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bioRxiv - Immunology 2019Quote: The three sets of primary mAb-treated mdPBMC were then incubated for 15 minutes at 4°C with anti-mouse IgG2a+b microbeads per the manufacturer’s instructions (Miltenyi Biotec). After incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... c-Kit+ cells were enriched using magnetic beads conjugated with an anti-c-Kit antibody (Miltenyi Biotec), transduced with a recombinant retrovirus by spinoculation ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... B cells were enriched with the B cell isolation Kit II (Miltenyi biotec) and stained with a recombinant ...
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bioRxiv - Immunology 2020Quote: Primary murine peritoneal cells were sorted into B-1a and B-1b cells using MACS B-1a selection kit (Miltenyi Biotec inc.). Sorted cells were cultured in RPMI1640 containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched using a B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) from spleens of immunized mice ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells were negatively sorted using the Pan B cell isolation kit (Miltenyi Biotec). Induction of germinal center B-cell differentiation was done following the protocol of T ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
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bioRxiv - Immunology 2019Quote: ... B cells were isolated from total splenocytes using naïve mouse B-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were isolated from PBMCs by using CD19+ B cell isolation kits (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... and total splenic B cells were purified using a negative B cell isolation kit (Miltenyi Biotec). Purity of enriched B cells was determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Immunology 2019Quote: ... Total B-cells were isolated by negative selection with the Memory B-cell Isolation Kit (Miltenyi Biotec). Memory-enriched B cells fractions were isolated by negative selection following incubation of total ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Cancer Biology 2023Quote: ... B cells were immunomagnetically separated using the MACS mouse B cells isolation kit (Miltenyi Biotec, Auburn, CA) and L.S ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were then isolated with Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec). Resting or stimulated CD4 T cells were nucleofected with the MPRA plasmid library using at least 6 technical replicates for each sample ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec) and IL-2 (10ng/ml ...
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bioRxiv - Bioengineering 2022Quote: ... in DPBS) and 2 μL of CD34-PE antibody (Miltenyi Biotec). Cells were then incubated at 4°C for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... B cells were isolated by negative selection using the B Cell Isolation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). The procedure was carried out following the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Immunology 2022Quote: Engineered mouse B cells were analyzed by double-staining with FITC labeled anti-mouse IgM antibody (Miltenyi Biotec, 130-095-906) and a SOSIP protein labeled with APC using a fluorochrome using the Lightning-Link (R ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Neuroscience 2022Quote: ... microbeads containing antibodies directed against ACSA-2 (130-097-678, Miltenyi Biotec) were used.
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bioRxiv - Biochemistry 2023Quote: ... 2 μL of conjugated antibody (anti CD119-PE, Miltenyi Biotec cat # 130125874) or isotype control (IgG1-PE ...
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bioRxiv - Immunology 2019Quote: ... Murine B cells were enriched by negative selection of IgMpos cells with a mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
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bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2023Quote: Memory B cells and naïve B cells were isolated from RosetteSep pre-sorted total B cells using MACS cell sorting from Miltenyi Biotec. Memory B cells were obtained via positive selection using the Milteny Memory B cell isolation kit ...
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bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Immunology 2022Quote: ... or specifically using two pools of Spike peptides covering the C- and N-terminal (PepTivator SARS-CoV-2 Prot_S1 and PepTivator SARS-CoV-2 Prot_S+) (Miltenyi Biotec Ref. 130-126-701& 130-126-700), Peptide pools were used at a final concentration of 1 μg/ml each peptide ...