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Citations for Miltenyi Biotec :
251 - 300 of 1301 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Immunology 2022Quote: ... Naïve B cells were then purified by negative selection using anti-CD43 microbeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by B cell enrichment using anti-CD19 magnetic beads (Miltenyi Biotec, 130-050-301) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Magnetic columns were used to deplete non-B cells according to standard protocol (Miltenyi Biotec). Cells in effluent were further labeled with NP-PE ...
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bioRxiv - Immunology 2021Quote: ... we purified B cells (Fig. 1D) by positive selection using magnetic beads (MACS, Miltenyi Biotec) conjugated with a monoclonal anti-mouse B220 antibody (clone ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were isolated from 16 healthy individuals and enriched for CD19+ B cells (Miltenyi Biotec). Two times 2.5 × 105 of naive B cells (CD20+IgM+IgD+IgG− ...
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bioRxiv - Immunology 2021Quote: ... B cells were excluded from whole splenocytes by incubating with anti-CD19 beads (Miltenyi Biotec) or by using the EasySep™ mouse CD19 positive selection kit (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
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bioRxiv - Immunology 2023Quote: ... The cells were subsequently processed using the Pan B Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Synthetic Biology 2020Quote: ... PBMC were isolated from a Buffy Coat from healthy volunteer donors using Ficoll gradient centrifugation and human T cells were isolated using the Human Pan T cell isolation kit (Miltenyi Biotec). Human T cells were cultured in supplemented RPMI media as described for cell lines above ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Physiology 2024Quote: Primary human monocytes were isolated from healthy human blood samples using CD14-magnetic beads (cat. no.: 130-050-201, Miltenyi Biotec). In brief ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Splenic CD4+ T cells or CD19+ B cells were positively selected using immunomagnetic MicroBeads (Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec). Cells were washed twice with 5 ml FACS buffer with 3 mM EDTA and adjusted to a cell density of 1-2×106 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
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bioRxiv - Microbiology 2021Quote: ... and B-cells were affinity-purified using CD19 microbeads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >95% pure B-cell populations ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2020Quote: ... was added to B cells and then analyzed by flow cytometry (MacsQuant Analyzer 10; Miltenyi Biotec,). The double labeling procedure allows a distinction into living (Annexin V −/PI−) ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Microbiology 2024Quote: CD19+ B cells were purified by positive magnetic separation using CD19 MicroBeads (Miltenyi Biotec, Gaithersburg, MD) and MS or LS columns ...
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bioRxiv - Immunology 2022Quote: ... using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... and then pelleted prior to resuspension in MB containing anti-mouse IgG2a+b MicroBeads (Miltenyi Biotec) and incubation for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Human IL-11 (Miltenyi Biotec ref# 130-094-623) and human IL-1β (Enzo Life Sciences ref# ALX-522-056 ...
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bioRxiv - Systems Biology 2019Quote: ... Human cells expressing CD45 (Miltenyi Biotec, 130-045-801) or CD235a (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... digested enzymatically (Miltenyi Biotec Whole Skin Dissociation Kit, human) for 2 hours and further dispersed using the Miltenyi gentleMACS Octo Dissociator ...
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bioRxiv - Microbiology 2022Quote: ... PE-conjugated anti-human CD11b (clone REA713, Miltenyi Biotec), BV786-conjugated anti-human CD4 (clone SK3 ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... using human CD14 Microbeads and LS columns (Miltenyi Biotec). Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CD133-PE antibody were purchased from Miltenyi Biotec. Human CD22 antibody were obtained from Biolegend ...
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bioRxiv - Physiology 2019Quote: ... Anti-human SLAMF6 (REA) was purchased from Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2019Quote: ... CD56+ NK cells (mouse anti-human CD56, Miltenyi Biotec) and CD19+ B cells (mouse anti-human CD19 ...
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bioRxiv - Immunology 2020Quote: ... and human M-CSF (100 ng/ml; Miltenyi Biotec) were added ...
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bioRxiv - Immunology 2021Quote: ... or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 50 U/mL human IL-15 (Miltenyi Biotec) for three days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 50 U/mL human IL-15 (Miltenyi Biotec). Monocytes were isolated from PBMCs by anti-human CD14 (REA599 ...
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bioRxiv - Bioengineering 2024Quote: ... 100 U/ml human IL-2 IS (Miltenyi Biotec) and 1 ng/ml IL-15 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... with added 100ng/mL Human GM-CSF (Miltenyi Biotec), and 100ng/mL Human IL-4 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec), recombinant human Flt3-ligand (Miltenyi Biotec) ...