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Citations for Miltenyi Biotec :
951 - 1000 of 1544 citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
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bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... 2mM ethylenediaminetetracetic acid (EDTA) and PBS and incubated with either CD133-APC or CD133-VioBright FITC antibodies (both monoclonal mouse, Miltenyi Biotec, dilution 1:500) and FcR blocking reagent (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were suspended in a 0.5% NGS solution then incubated with anti-PDGFRα or anti-O4 coupled-beads (Miltenyi Biotec; ref 130-094-543 and 130-096-670). Unbound bead-coupled antibodies were washed away by centrifugation ...
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bioRxiv - Genomics 2024Quote: ... The EGFR-Hofbauer/fibroblast fraction was washed and incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ phycoerythrin (PE)-conjugated anti-CD10 antibody (anti-CD10-PE, Miltenyi Biotec, #130-114-5-2, lot #5190109168). The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... detected with 1:2500 diluted anti-GFP HRP conjugated antibodies (Miltenyi Biotec Cat# 130-091-833 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec), then stained with the I-A/E-APC antibody (MHCII ...
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bioRxiv - Neuroscience 2020Quote: ... with LS columns and Anti-PE MicroBeads (Miltenyi Biotec 130-048-801) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Cells were activated with soluble human anti-CD3 (clone OKT3, Miltenyi Biotec) and human anti-CD28 (clone 15E8 ...
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bioRxiv - Immunology 2021Quote: ... cell suspensions were counted and stained with anti-CD45 microbeads (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: Human and mouse DCs were stained with the following antibodies from Miltenyi Biotec or as specified: Vioblue-conjugated anti-human CD86 (clone FM95, Miltenyi Biotec), PE-conjugated anti-human CD83 (clone REA714) ...
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bioRxiv - Immunology 2021Quote: ... and stained with APC-conjugated anti-Granzyme B (clone REA226, Miltenyi Biotec). Cell viability was assessed by LIVE/DEAD staining according to the manufacturer’s instruction (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-MHC Class II (Miltenyi Biotec, 130-102-139, clone M5/114.15.2), anti-ICAM-1 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were purified using anti-CD4+ conjugated microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with anti-O4 Microbeads (130-094-543, Miltenyi Biotec) and magnetic separation was done using Multi-24 Column Blocks and the MultiMACS Cell24 Separator Plus (130-095-692 and130-098-637 ...
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bioRxiv - Physiology 2021Quote: ... PBMCs purification was performed using anti-human CD14 magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... anti-CD158b (KIR2DL2/DL3) APC vio 770 (clone REA 1006, Miltenyi Biotec), CD85j (ILT2 or CD94 ...
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bioRxiv - Immunology 2022Quote: ... a mixture of PerCP-Vio700-anti-CD3 (130-119-656, Miltenyi Biotec), PECy7-CD4 (552775 ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 minutes and Anti-Biotin MicroBeads (Miltenyi Biotec, 130-090-485) for 10 minutes at 2-8°C ...
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bioRxiv - Biochemistry 2020Quote: ABCB1 co-immunoprecipitation analyses using anti-GFP micro beads (Miltenyi Biotec, Germany) were carried using microsomal fractions prepared from Agrobacterium-co-infiltrated N ...
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bioRxiv - Developmental Biology 2022Quote: ... or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443) per 107 total cells ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were first labeled with PE-conjugated anti-CD154 (5C8, Miltenyi Biotec) and CD154+ cells were then enriched using anti-PE magnetic beads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... by positive selection with anti-CD4 microbeads (Miltenyi Biotec Inc., Auburn, CA).
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bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Monocytes were labelled with anti-human CD14 antibody (clone REA599, Miltenyi Biotec). Immature phenotype of neutrophils was assessed by surface CD10 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by magnetic labeling with an Anti-APC MicroBeads kit (Miltenyi Biotec). Cells were then applied to magnetic MS columns (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... in combination with anti-Biotin UltraPure MicroBeads (Miltenyi Biotec, 130-105-637). Single cells were counted using Luna-II automated cell counter (Logos Biosystems ...
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bioRxiv - Immunology 2021Quote: ... followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotec). Purity (>90% CD34+ cells ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with anti-IL-10-PE-conjugated antibody (Miltenyi Biotec). For all primary antibodies relevant isotype control antibodies were used ...
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bioRxiv - Immunology 2020Quote: ... anti-Major Histocompatibility Complex II (MHC-II-PE (Miltenyi Biotec, Clone: REA610); anti-Norepinephrine transporter (NET ...
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bioRxiv - Microbiology 2021Quote: ... They were activated by incubation with anti-biotin MACSiBead particles (Miltenyi Biotec) loaded with biotinylated anti-CD2 ...