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Citations for Miltenyi Biotec :
601 - 650 of 909 citations for Leucine Rich Repeats And Immunoglobulin Like Domains Protein 1 LRIG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Nonspecific binding was blocked by using 1% Fc block (Miltenyi Biotec). Cells were stained with HLA-A2 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
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bioRxiv - Cell Biology 2024Quote: ... by running mouse lung 1-2 program on GentleMACS (Miltenyi Biotec). Lung pieces were then digested by shaking (∼150 rpm ...
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bioRxiv - Immunology 2022Quote: ... and erythrocyte depletion cocktail (#130-098-196, Miltenyi Biotec, 1:50) was added ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and FITC-Labeling Reagent (Miltenyi Biotec, Cat # 327806, 1:50 dilution). Composition analysis of enriched iSCs was performed using anti- ITGB4 (BD Cat # 744150 ...
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bioRxiv - Molecular Biology 2023Quote: ... PE-conjugated anti-O4 (Miltenyi Biotec, 130-117-507, 1:50), and APC-conjugated anti-ACSA-2 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti- CD276-FITC (Miltenyi Biotec, 130-118-697, 1:50) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... or CD86 (1:100, Miltenyi Biotec Cat# 130-109-130, RRID:AB_2659403), PE-conjugated anti-CD45RA (1:100 ...
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bioRxiv - Genomics 2023Quote: ... Multi Tissue Dissociation Kit 1 and gentleMACS Octo Dissociator (Miltenyi Biotec) were used for dissociation of the tissue into single-cell suspension with a reduced amount of enzyme R (25% of the regular amount) ...
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bioRxiv - Cancer Biology 2024Quote: ... Rae1 (REA723) and Ly6a/Sca-1 (REA422) purchased from Miltenyi Biotec. Single living cells were gated according to size and granularity in FSC-A ...
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bioRxiv - Neuroscience 2024Quote: ... using the anti-CD11b (1:50, 130-113-806, Miltenyi Biotec) and anti-CD45 (1:50 ...
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Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spreadbioRxiv - Microbiology 2024Quote: ... and 1% penicillin-streptomycin in gentleMACS™ M tubes (Miltenyi Biotec) to generate a 10% tissue homogenate.
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Molecular Biology 2019Quote: For isolating the SSEA1 positive cells from reprogramming intermediates at day6 and day9 we used magnetic microbeads conjugated to anti-SSEA1 antibody (MACS Miltenyi Biotec #130-094-530) as per manufacture instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... also known as LPEC-1 and -6 in previous studies) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) 10-13 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
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bioRxiv - Immunology 2021Quote: B cells were purified from splenocytes by positive selection using magnetic beads-conjugated monoclonal anti-mouse B220 antibody (clone: RA3-6B2, Miltenyi Biotec, Bergisch Gladbach, Germany). Isolated B cells (5 × 105/mL ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2022Quote: ... and efficiently depleted of non-target cells by addition of magnetic microbeads conjugated to mouse anti-biotin monoclonal IgG1 antibodies and passage through LS ferromagnetic columns (Miltenyi Biotec, 130-042-401). The flow through ...
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bioRxiv - Physiology 2023Quote: ... the cells were mixed with CD45 antibody-coated magnetic beads (CD45 MicroBeads, 130-052-301) and then passed through a MACS LD column (Miltenyi Biotec, 130-042-901) on a MidiMACSTM separator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
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bioRxiv - Pathology 2023Quote: ... CD14 + monocytes were then isolated from the PBMCs by positive selection using magnetic beads conjugated to anti-CD14 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Cancer Biology 2023Quote: CD40 stimulation medium were prepared by incubation of 40 uL of CD40 ligand and 40 uL of cross-linking antibody (Miltenyi Biotec 130-106-196) mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... GLAST (1:500, mouse monoclonal; Miltenyi Biotec cat. no. 130-095-822), or NFIA (1:200 rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec, #130-092-613, 1:100), Anti-Biotin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... in mTeSR™1 medium supplemented with StemMACS™ Y27632 (Miltenyi Biotec). Once the cells reached greater than 95% confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... and CD338-PE (ABCG2-PE, Miltenyi Biotec, 130-105-010, 1:40) followed by 1hour incubation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... at a 1:100 dilution and human FcR blocking reagent (Miltenyi Biotec) at a 1:20 dilution on ice for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... containing 1 mL PBSbefore homogenizationwith a tissue dissociator (Oligo-Macs, Miltenyi Biotec). The samples were serially diluted and plated on cetrimide agar.
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bioRxiv - Immunology 2023Quote: ... and blocked with PBS supplemented with 1% Fc Block (MACS, Miltenyi Biotec) and 0.5% BSA for 15 minutes at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... 25 ng mL−1 macrophage–colony stimulating factor (w/v; Miltenyi Biotec), and 100U mL−1 penicillin/streptomycin (w/v ...
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bioRxiv - Immunology 2020Quote: Peritoneal B1a cells were obtained through negative magnetic-activated cell separation with a cocktail of antibodies depleting other than B1a cells achieving more than 90% purity in isolation (Miltenyi Biotec B.V., Utrecht, The Netherlands). B1a cells were subsequently counted and plated in IMDM medium (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were positively selected using anti-CD4 and CD8 antibodies through the Prodigy TCT program on a CliniMACS Prodigy (Miltenyi Biotec, Cat# 200-075-301). After selection ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec). Primary cell cultures were maintained in the presence of mouse embryonic fibroblasts (STO ...
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bioRxiv - Cell Biology 2020Quote: Astrocytes were purified using the Anti-GLAST (ACSA-1) MicroBead Kit (Miltenyi biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry was done using anti-CD133/1-PE (Miltenyi Biotec, clone AC133) and anti-GD2-APC (Biolegend ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-cMyc-FITC (1:50, Miltenyi Biotec, Somerville, MA, #130-116-485) at 4°C for 45 min ...
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bioRxiv - Neuroscience 2019Quote: ... human recombinant anti-CD11b/c (1:50) (#130-120-288, Miltenyi Biotec, Germany). Secondary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-cardiac troponin-T-APC (Miltenyi Biotec, 130-120-403, 1:50) in blocking buffer (PBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-cMyc-FITC (1:50, Miltenyi Biotec, Somerville, MA, #130-116-485) at 4°C for 45 min in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... RRID:AB_1227553) or anti- granulocytes (1:100, Miltenyi Biotec Cat# 130-108-084, RRID:AB_2651886), PerCP5.5-conjugated anti-CD8 (1:100 ...