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Citations for Miltenyi Biotec :
501 - 550 of 1301 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... using the human specific CD235a+ glycophorin A MicroBeads (Miltenyi Biotec Inc, 130-050-501). Purified CD235a+ were evaluated for erythroid maturation and enucleation (Table S3) ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were isolated by MACS using the human CD14+ isolation kit (Miltenyi Biotec). CD14+ monocytes were differentiated into DCs over 6 days by culturing in RPMI (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
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bioRxiv - Immunology 2022Quote: ... B cells were depleted from OTII splenocytes by magnetic sorting using CD45R (B220) MicroBeads according manufacturer’s instructions (Miltenyi Biotec cat. 130-049-501). For experiments involving cell division analysis ...
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bioRxiv - Bioengineering 2022Quote: Omicron RBD-specific memory B cells were isolated from pre-enriched memory B cells by magnetic positive selection according to the manufacturer’s protocol (Miltenyi Biotec, 130-095-838). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Systems Biology 2022Quote: ... and T and B cells were depleted by using CD19 and CD3 MicroBeads (Miltenyi Biotec Cat#130-097-055 and #130-097-043) to enrich for myeloid cells ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2020Quote: ... and spike proteins (Prot_S) were purchased from Miltenyi Biotec. Optimal peptides for the manufacture of HLA class I tetramers were synthesized at >95% purity by Peptides & Elephants GmbH ...
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bioRxiv - Cell Biology 2019Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Cell Biology 2024Quote: The μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) was used to pull down proteins associated with the GFP-tagged protein ...
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bioRxiv - Genomics 2020Quote: ... Structures were enzymatically dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec) with a gentleMACS™ Octo Dissociator (with heaters ...
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bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
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bioRxiv - Cell Biology 2021Quote: Human neutrophils were isolated on a MACS LS column system (Miltenyi Biotec #130-042-401), enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601 ...
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bioRxiv - Immunology 2022Quote: ... resting NK cells were obtained using the human NK cell isolation kit (MACS Miltenyi Biotec), following manufacture’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... Human monocytes were isolated from PBMC using a magnetic-based monocyte isolation kit (Miltenyi Biotec) and differentiated into macrophages with GMCSF (50 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Systems Biology 2020Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (MACS Miltenyi Biotec) and LS Columns (Miltenybiotec) ...
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bioRxiv - Immunology 2019Quote: ... human CAR T cells were sorted for CD34+ (CD34 MicroBead Kit UltraPure, MACS, Miltenyi Biotec) CAR T cells and expanded for up to 15 days in the presence of human IL-2 ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... T cells were stimulated and expanded using TransAct Human T cell activation reagent (Miltenyi Biotec). For transduction ...
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bioRxiv - Immunology 2019Quote: ... density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201, Miltenyi Biotec). Monocytes were incubated for 7 days with RPMI-1640 (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... HDMECs were then isolated using a human CD31 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... CD8 or CD4 T cells were purified from human PBMCs using isolation kits (Miltenyi Biotec) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified using the human Naïve Pan T Cell Isolation Kit (Miltenyi Biotec) and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... The freshly isolated PBMCs were subsequently cultured in human NK MACS® medium (Miltenyi Biotec) for 2 to 3 weeks to ensure optimal in vitro expansion of NK cells ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with FcR-blocking reagent and human CD34 Microbeads (Miltenyi Biotec, Gaithersburg, MD) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... One quarter of the of the cell suspension volume human anti-fibroblast Microbeads (Miltenyi Biotec) were added for 30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... human according to manufacturer instructions and the GentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) using custom settings ...
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bioRxiv - Cell Biology 2023Quote: ... CD14+ monocytes were then obtained by positive magnetic isolation using human CD14 microbeads (Miltenyi Biotec) and a MACS LS column with a QuadroMacs™ separator (Miltenyi Biotech) ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Developmental Biology 2024Quote: A truncated human CD4 sequence (corresponding to the sequence identified by hCD4 MicroBeads, Miltenyi Biotec) was cloned into a pCS2+ vector containing an SP6 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested using the Tumor Dissociation Kit for human tissue (Miltenyi Biotec: #130-095-929) on a gentle MACS Octo Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: B cells were purified by negative selection from single-cell suspensions from spleen using magnetic separation B cell isolation kit (Miltenyi Biotec 130-090-862). Purified cells were cultured for 4 days in RPMI (Corning ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and CD19+ B cells were primarily sorted from PBMCs of convalescents and vaccinees by CD4 and CD19 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively ...
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were eluted with 100 μL elution buffer (Miltenyi Biotec). Proteins were resolved in 8% SDS-PAGE gels described above.
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bioRxiv - Immunology 2020Quote: Protein A beads and µ columns were from Miltenyi Biotec Ltd. ...
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bioRxiv - Biochemistry 2019Quote: ... and subsequent CD14+ positive selection using the MACS Miltenyi Biotec Human CD14 microbead protocol (Miltenyi Biotec). CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were labeled with human CD11b-APCVio770 and CD45-phycoerythrin (PE) (Miltenyi Biotec, Gladbach, Germany), and flow cytometry was performed as described previously (38) ...