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Citations for Becton, Dickinson and Company :
1 - 50 of 1974 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC (BD), Streptavidin-PE (BD) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Streptavidin-Horseradish Peroxidase (HRP) (BD #557630) and AEC chromogen substrate (BD #551951 ...
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bioRxiv - Immunology 2022Quote: ... CD169+) were flow-sorted prior to infection with SARS-CoV-2 using FACSAria Fusion (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
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bioRxiv - Immunology 2021Quote: ... with horseradish peroxidase(HRP)-conjugated Streptavidin (BD Biosciences) for 1 hour (h) ...
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bioRxiv - Neuroscience 2022Quote: ... they were probed with horseradish peroxidase-Streptavidin (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
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bioRxiv - Immunology 2022Quote: ... 2% Fc block (BD), and fixable viability dye eFluor 450 (ebiosciences) ...
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bioRxiv - Physiology 2020Quote: ... and horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences, 554066; 1:300). The HRP activity was visualized with diaminobenzidine staining ...
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bioRxiv - Immunology 2020Quote: ... and then detected with streptavidin conjugated horseradish peroxidase (554066, BD biosciences). Plates were developed using TMB (Dako ...
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bioRxiv - Immunology 2022Quote: ... A total of 100 μl per well of horseradish peroxidase-conjugated streptavidin (BD Biosciences) diluted in PBS containing 0.1% BSA was then added and incubated for 1 hr at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 2% FCS and 10% Brilliant Stain Buffer (BD) were then added directly to cells and incubated for a further 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulins were detected with horseradish peroxidase (HRP) goat anti-mouse Ig (BD Pharmingen, 1/2000 diluted), IgM ...
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bioRxiv - Immunology 2019Quote: ... followed by streptavidin-conjugated horseradish peroxidase (SA-HRP) and TMB substrate (both BD Bioscience, San Diego, CA). Anti-mouse Ig(H+L ...
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bioRxiv - Immunology 2023Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... fixed with 2% paraformaldehyde with Fc block (BD Biosciences) before and after ...
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bioRxiv - Immunology 2023Quote: ... afterward kept in staining buffer (2% FCS, BD Biosciences). Surface proteins were stained for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... IL-1β was detected using biotinylated anti IL-1β (eBioscience) and streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected using an o-phenylenediamine hydrochloride (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: SVC were resuspended in 2% Fc Block (553142, BD Pharmingen) and blocked for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 2*106 cells were incubated with Fc block (BD Pharmingen) and subsequently stained for surface markers with fluorophore-conjugated antibodies for 30 minutes on ice.
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bioRxiv - Cancer Biology 2023Quote: ... in PBS supplemented with 2% FCS and iMag (BD Biosciences). After depletion ...
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bioRxiv - Immunology 2022Quote: ... followed by washing and incubation for 30 mins at room temperature with horseradish peroxidase (HRP)-streptavidin (BD, Cat. 554066) and Amplex red substrate for 45 mins at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (HRP-linked anti-mouse or anti-rabbit IgG; BD Biosciences) at RT for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µL of CD16/CD32 Fc Block antibody (BD Biosciences 553141) was added for 10 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... and suspended in 2% FBS/DPBS buffer with Fc Block (BD) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... IL-1β was then detected with a secondary biotinylated antibody (eBioscience) followed by streptavidin conjugated to horseradish peroxidase (BD Biosciences). Peroxidase activity was detected by o-phenylenediamine hydrochloride (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
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bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2021Quote: ... + 2% FBS with Fc block (1:100 dilution, clone 2.4G2; BD Biosciences) along with the following antibodies (all from BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... Tissues were blocked with 2% Fc block (BD Pharmingen Cat. No. 553142) with 5% normal goat serum in 1X PBS at room temperature for one hour ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice in PBS + 2% FCS + 2 mM EDTA and incubated with mouse FcBlock (BD Biosciences), Epcam-APC (CD326 Monoclonal Antibody (G8.8) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in PBS + 2% FCS and an Influx cell sorter (BD) was used.
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bioRxiv - Immunology 2021Quote: ... and cells were suspended in 2% FBS/DPBS buffer with Fc Block (BD) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were suspended in 2% FBS/DPBS buffer with Fc Block (BD Biosciences) for 10 minutes15 ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 cells were incubated with 50 µL Fc Block (BD Biosciences, cat #553142) that was pre-diluted 1:50 in FACS buffer (PBS (Gibco/Thermofisher scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1-2 x 106 BM cells were incubated with human Fc BlockTM (BD, 564219) for 10 minutes at RT ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...
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bioRxiv - Cell Biology 2021Quote: ... sheep anti-human CD31 1:500 (BD AF806) and chicken anti-GFP 1:1000 (Abcam ab13970) ...
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2021Quote: ... Spike protein conjugated to Alexa 647 and Spike protein conjugated to Alexa 488 and Brilliant Staining buffer (BD Horizon). Samples were then incubated on ice for an additional 30 minutes before they were washed twice with P2 and fixed in a final concentration of 1% paraformaldehyde for 15 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL2/L3/S2 (BD, Clone CH-L: BrilliantViolet605, FITC), KIR3DL1/S1 (Miltenyi ...