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Citations for Becton, Dickinson and Company :
1 - 50 of 1269 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... spike and SARS-CoV-1 RBD were incubated with Streptavidin-APC (BD), Streptavidin-PE (BD) ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Immunology 2022Quote: ... CD169+) were flow-sorted prior to infection with SARS-CoV-2 using FACSAria Fusion (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Spike-RBD binding was measured by flow cytometry on a BD FACSCelesta (BD Biosciences) and analyzed with FlowJo software.
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated prefusion-stabilized spike (S-2P) and spike subdomain (NTD, RBD) probes were produced as previously described79 and conjugated to streptavidin-labeled dyes (BD) to yield the following and streptavidin-conjugated B cell probes ...
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bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... RBD SA-BV650 (BD), and S-2P SA-APC (BD) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for the spike ELISA) for 5 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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Chromosome fissions and fusions act as barriers to gene flow between Brenthis fritillary butterfliesbioRxiv - Evolutionary Biology 2022Quote: ... daphne individual (FR_BD_1329; Table S1) was used for chromatin conformation capture (HiC ...
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bioRxiv - Immunology 2021Quote: ... Beta-RBD with BUV661-streptavidin (BD Biosciences), and Delta RBD with PE-streptavidin (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and RBD to streptavidin-PE (BD Biosciences, 554061) and streptavidin-AF647 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... and RBD to streptavidin-PE (BD Biosciences, 554061) and streptavidin- AF647 (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... RBD-Alexa 647 in brilliant stain buffer (BD) for 20min ...
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bioRxiv - Immunology 2021Quote: ... and RBD to streptavidin-PE (BD Biosciences, 554061) and streptavidin-AF647 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and RBD to streptavidin-PE (BD biosciences, 554061) and streptavidin-Alexa Fluor 647 (AF647 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 infected cells were fixed in 2% formaldehyde for 30 mins and permeabilized with BD Perm/Wash buffer (BD Biosciences, 554723). The primary antibody used was sheep anti-SARS-CoV-2 nucleocapsid antibody (DA114 ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 MR1-5-OP-RU-APC Tetramer magnetic bead enriched spleen (n= 2) or unenriched colon (n= 2) single cell suspensions were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). MAIT cells (MR1-5-OP-RU-Tet+TCRβ+CD3+ ...
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bioRxiv - Immunology 2022Quote: ... RBD was conjugated to streptavidin-PE (BD Biosciences, 554061) and streptavidin-AF647 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... RBD was conjugated to streptavidin-PE (BD Biosciences, 554061) and streptavidin-AF647 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and SD1-RBD to streptavidin-PE (BD biosciences, 554061) and streptavidin-Alexa Fluor 647 (AF647 ...
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bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...
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bioRxiv - Immunology 2021Quote: ... Spike protein conjugated to Alexa 647 and Spike protein conjugated to Alexa 488 and Brilliant Staining buffer (BD Horizon). Samples were then incubated on ice for an additional 30 minutes before they were washed twice with P2 and fixed in a final concentration of 1% paraformaldehyde for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... WT RBD was conjugated to streptavidin-PE (BD Biosciences, 554061) and streptavidin-AF647 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Delta RBD was conjugated to streptavidin-PE (BD Biosciences, 554061) and Omicron BA.1 RBD (ACROBiosystems ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Immunology 2020Quote: ... VCAM1-BB515 (1:20, clone S1-10C9, #9093693, BD Biosciences), CD103-PerCP-Cy5.5 (1:50 ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Immunology 2023Quote: ... up to 2 x106 enriched or unenriched cells were labeled with an oligonucleotide-tagged antibody sample tag (BD Biosciences). From infant thymus and PBMC donors up to 5 populations were sorted after doublet ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated Ovalbumin and RBD were conjugated to streptavidin-BV711 (BD biosciences, 563262) and SD1-RBD to streptavidin-PE (BD biosciences ...
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bioRxiv - Immunology 2024Quote: ... RBD-647+ B cells were sorted on a FACS Aria (BD Biosciences) in the Robert H ...
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bioRxiv - Genomics 2023Quote: ... CD34+ cells (gating as per Fig. S1) were FACS-sorted (BD FACSMelody ...
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bioRxiv - Immunology 2023Quote: ... p-MPM2 (Table S1) in BD Perm/WashTM buffer (BD biosciences) containing 2% FCS for 30 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized with isoflurane 1% to 2% v/v oxygen and immunized subcutaneously with 200 μg myelin oligodendrocyte glycoprotein 35–55 (MOG35–55) peptide (peptides & elephants) in complete Freund’s adjuvant (BD) containing 4 mg ml−1 Mycobacterium tuberculosis (BD) ...
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bioRxiv - Immunology 2021Quote: ... or A549-Spike cells in the presence of GolgiStop (BD Biosciences) in a V-bottomed 96-well plate in complete RPMI-1640 media at 37°C under 5% CO2 for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...