Labshake search
Citations for Becton, Dickinson and Company :
1 - 50 of 5651 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
-
bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained for 30 min on ice with biotinylated recombinant HAs and PD-1-BB515 (EH12.1, BD Horizon, 1:100) diluted in P2 ...
-
bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were tetramerized with streptavidin-PE (BD) at a 4:1 molar ratio.
-
bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
-
bioRxiv - Immunology 2019Quote: ... Active HIV-1 infection was estimated by flow cytometry (BD LSRII, BD bioscience) as the percentage of eGFP-expressing ...
-
bioRxiv - Immunology 2019Quote: ... Active HIV-1 infection was estimated by flow cytometry (BD LSRII, BD bioscience) as the percentage of eGFP-expressing ...
-
bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... intracellular HIV-1-p24 staining was performed using the Cytofix/CytopermTM kit (BD #554714) using anti-HIV-1 p24-PE antibody (#6604667 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Prox1 and pErkT202/Y204 proteins were detected non-fluorescently using biotinylated goat anti-rabbit IgG (1: 400, BD Biosciences) followed by avidin-HRP and DAB detection (Vector labs ...
-
bioRxiv - Immunology 2024Quote: ... biotinylated anti-IgG1 (clone A85-1, BD Biosciences) was added at 1 µg/mL in 10% FBS in PBS ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: Purified HIV particles were fixed using by BD CytoPerm/Cytofix Solution and adhered to poly-L-lysine solution (SIAMG ...
-
bioRxiv - Microbiology 2020Quote: ... an HIV-blocking anti-CD4 antibody (BD Biosciences) was used ...
-
bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
-
bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... and (3) biotinylated anti-mouse CD184 (1:100; BD Biosciences). After incubation for 30 min ...
-
bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... biotinylated CD49d (BD Biosciences Cat# 557419 ...
-
bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells containing Atto488-tagged protein (530 nm) were detected by flow cytometry on a FACSCalibur (BD Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... rat anti-CD8 biotinylated (1:500, catalog no. 553028; BD Biosciences), rabbit anti-LAG3 (1:100 ...
-
bioRxiv - Immunology 2023Quote: ... the biotinylated forms of anti-mouse IgG1 (BD Biosciences; A85-1) or anti-mouse IgG2b (BD Biosciences ...
-
bioRxiv - Immunology 2020Quote: ... For HIV detection cells were incubated with Cytofix/Cytoperm solution (BD) for 15 minutes at room temp and washed with permeability wash (1% BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
-
bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated rat anti-mouse IgG2a antibody (1:300; BD Biosciences, Cat. 550332), or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L ...
-
bioRxiv - Cancer Biology 2020Quote: FITC-tagged AnnexinV (BD-Biosciences), PI(Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
-
bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
-
C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
-
bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
-
bioRxiv - Immunology 2020Quote: ... intracellular HIV-p24 staining was performed using the Cytofix/CytopermTM kit (BD #554714). Flow cytometry was performed using BD LSRFortessa or BD accuri c6 plus ...
-
bioRxiv - Immunology 2021Quote: ... reactivation of latent HIV-1 was determined by quantifying the percentage of GFP+ cells using the FACS ARIA and analyzed using FlowJo (BD Biosciences) software ...
-
bioRxiv - Immunology 2021Quote: ... Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063 ...
-
bioRxiv - Immunology 2022Quote: ... Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and immunostained with biotinylated antibodies as follows: anti-mouse IgG1 (1:100, BD Pharmingen 553441; A85-1), anti-mouse IgG2b (1:100 ...
-
bioRxiv - Bioengineering 2023Quote: ... Recombinant TNFα was purchased from R&D Systems (cat #: 210TA100/CF) and recombinant MCP-1 was purchased from BD Biosciences (cat # ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
-
bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
-
bioRxiv - Physiology 2022Quote: ... CD11c+ DCs were isolated using biotinylated anti-CD11c (1:200; clone HL3; BD Biosciences) and anti-biotin microbeads (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2019Quote: ... biotinylated anti-mouse IgG2a (BD PharMingen), and anti-mouse IgE (BioLegend) ...
-
bioRxiv - Immunology 2021Quote: ... IgD-biotinylated (IA6-2, BD Bioscience), IgM-FITC (G20-127 ...
-
bioRxiv - Developmental Biology 2022Quote: ... and biotinylated secondary antibody (BD, NJ) and fluorochrome-conjugated streptavidin (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2021Quote: ... HIV replication was measured using a BD Accuri C6 Flow Cytometer (BD Bioscience, USA) as percent HIV-iRFP670 expressing cells in HIV-guide RNA vs ...
-
bioRxiv - Microbiology 2022Quote: ... HIV infected cells and cell viability were both analyzed by Flow cytometry (BD LSRFortessa) and quantified using FlowJo™ v10.
-
bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
-
bioRxiv - Molecular Biology 2019Quote: ... (4) biotinylated anti-streptavidin diluted 1:50 and IdU primary antibody (mouse anti-BrdU, BD Biosciences 347580 ...
-
bioRxiv - Immunology 2023Quote: ... Slides were stained with biotinylated anti-mouse CD35 (diluted in 1:100) (8C12, BD Bioscience), APC conjugated anti-mouse CD3 (1:100 ...
-
bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.