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Citations for Becton, Dickinson and Company :
1 - 50 of 592 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
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bioRxiv - Neuroscience 2019Quote: ... and enhanced green fluorescent protein cDNAs (eGFP; BD Bioscience) as described previously (Raingo et al. ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibody or recombinant protein biding on fluorescent nanobeads were directly counted and measured by BD FACSCelesta™ flow cytometer.
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bioRxiv - Immunology 2023Quote: ... The green fluorescent protein (GFP) signal from cells was measured using a FACSVerse (BD). To measure autophagic flux ...
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bioRxiv - Microbiology 2019Quote: ... Flow cytometric analysis was performed by Green Fluorescent Protein (GFP) on FACSAria Flow Cytometer (BD Biosciences). FACS data was analyzed with FlowJo software (TreeStar Inc.).
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bioRxiv - Cancer Biology 2021Quote: ... CAR-T cells were stained with fluorescent tagged anti-CD3 (BD Biosciences, Cat#557851), anti-NGFR (Miltenyi Biotech ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 344SQ murine lung adenocarcinoma cells expressing firefly luciferase and green fluorescent protein (in 50 μL of 1:1 mix of HBSS and BD Matrigel) were injected into the left lung of 8 weeks old female 129/Sv mice via intrapulmonary injection as described previously (50) ...
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bioRxiv - Microbiology 2020Quote: ... Green fluorescent beads (3.5 micron) and 5 micron Accudrop beads from BD biosciences were used to calibrate the instrument for laser alignment ...
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bioRxiv - Molecular Biology 2022Quote: ... Viral production titers were assessed on HT1080 cells with serial dilutions of a lentivector expressing GFP and scored for green fluorescent protein (GFP) expression by flow cytometry analysis on a BD FACSVerse (BD Biosciences, Le Pont de Claix, France).
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bioRxiv - Cell Biology 2021Quote: ... bound proteins were analyzed by western blotting using anti-His monoclonal antibodies (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... living (green fluorescent) cells were separated from dead (red fluorescent) cells and isolated by FACS on a BD FACSAria™ Fusion (BD Biosciences Franklin Lakes, NJ) using an excitation with a 488 nm laser and the emission collected using a 530/30 band-pass filter for living (calcein AM-labelled ...
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bioRxiv - Microbiology 2021Quote: ... Five-to-ten microliters of green fluorescent reference beads of 3.0-3.4 μm (Rainbow beads, BD Biosciences) were added to each flow cytometry tube prior to acquisition to calculate bacterial concentration ...
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bioRxiv - Cell Biology 2019Quote: ... Cells containing Atto488-tagged protein (530 nm) were detected by flow cytometry on a FACSCalibur (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... cells expressing red fluorescent protein were enriched by FACS Aria Fusion SORP (BD Bioscience) and plated into a dish at a very low density ...
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bioRxiv - Cancer Biology 2020Quote: FITC-tagged AnnexinV (BD-Biosciences), PI(Sigma ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing eGFP-tagged huntingtin proteins were harvested and eGFP expression was analyzed on an LSR Fortessa (BD Pharmingen) flow cytometer for PulSA analysis to detect protein aggregates ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... cells expressing TagRFP-tagged proteins were flow-sorted using a BD Influx cell sorter (BD Bioscience, San Jose, CA). TagRFP emission was triggered by 561 nm laser ...
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bioRxiv - Systems Biology 2019Quote: Analysis of fluorescent protein reporter expression was performed with a BD LSRII flow cytometer (BD Biosciences) equipped with a high-throughput sampler ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Biophysics 2020Quote: ... Positive cells were amplified under antibiotic selection pressure and sorted for low or intermediate expression level of the fluorescently tagged protein on an Aria II Fluorescence Activated Cell Sorter (BD). Each sorted population was then used for pilot experiments to determine the lowest possible expression level required for optimal imaging conditions ...
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bioRxiv - Physiology 2023Quote: ... livers protein lysates were incubated with fluorescent substrate Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) at 37°C and the reaction was monitored for at least 4 h to track changes in fluorescence (λ excitation of 390 nm and λ emission of 510 nm) ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Immunology 2023Quote: ... and recombinant IFNγ (BD Pharmingen) as a standard ...
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bioRxiv - Immunology 2020Quote: ... Rainbow Fluorescent Particles (BD Biosciences) and applications settings in BD FACSDiva v7.0 were used to correct for day-to-day variations in instrument performance ...
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bioRxiv - Immunology 2021Quote: ... using a human IL-1β enhanced-sensitivity flex set (BD Biosciences). Undiluted cell supernatant was stained according to the manufacturer’s instructions and run on a BD FACS Canto II ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Immunology 2021Quote: ... FNA samples were stained in P2 for 30 minutes on ice with biotinylated and Alexa Fluor 647 conjugated recombinant soluble Spike proteins as well as PD-1 BB515 (EH12.1, BD Horizon). Cells were then washed twice with P2 and stained with IgG BV480 (goat polyclonal ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... and the amounts of recombinant ACE2-GFP and dACE2-GFP proteins in lysates were quantified by densitometry analysis (Imagelab, BD Biosciences) of Western blots ...
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bioRxiv - Cell Biology 2022Quote: ... Green fluorescent protein (GFP) positive cells in HRasL61 with GFP transfected cells were sorted using flow-cytometry (FACS Aria II; BD Bioscience).
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bioRxiv - Neuroscience 2023Quote: Expression of the different fluorescent proteins (ORFs) of the stable GEFRI2 line were measured by High Throughput LSR Fortessa (BD Biosciences) fluorescence-activated cell sorter (FACS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected with 5 mg/mL puromycin and sorted based on red fluorescent protein (RFP) by fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Immunology 2022Quote: ... CD69 BV711 (Clone HI.2F3) (BD Cat. 740664), CD103 PE-CF594 (Clone M290 ...
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bioRxiv - Immunology 2023Quote: ... IL17A and IL13 concentration was measured using Enhanced Sensitivity CBA (BD Biosciences). The limit of detection was 274 fg/mL.
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bioRxiv - Immunology 2023Quote: ... IL5 and IL13 concentration was measured using Enhanced Sensitivity CBA (BD Biosciences). The limit of detection was 274 fg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Cancer Biology 2022Quote: ... Stable cells expressing fluorescent reporter proteins (mCherry or eGFP) were sorted using BD FACSAria Flow Cytometer (BD Bioscience, Mountain View, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... fluorescent GCs were purified using fluorescent small particle sorting (FSPS) on a customized SORP FACSAriaII (BD Instruments) equipped with a 70 μm nozzle running at 70 psi and a 0.1 μm filter for the sheath fluid to remove potential dust particles ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in supernatants were measured using Enhanced Sensitivity Flex Sets (BD Biosciences) for mouse IL-5 and IL-13 according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... A polyclonal population of cells expressing all 3 fluorescent proteins (mturquoise2, Venus and mCherry) was subsequently generated by two rounds of FACS (BD FACS Aria II).
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bioRxiv - Systems Biology 2022Quote: Recombinant human EGF was purchased from BD Biosciences (San Jose ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant human EGF was purchased from BD Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent activated cell sorting (FACS, BD, influx) sorted CD31-CD45-CD184+ cells were cultured in complete growth medium DMEM supplemented with 20% FCS and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent antibodies were purchased from from BD Biosciences ...
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bioRxiv - Systems Biology 2021Quote: ... and anti-active Caspase3- V450 tagged antibody)) were procured from BD Biosciences.
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bioRxiv - Neuroscience 2023Quote: ... Alexa-488 tagged cytochrome c antibody (BD Biosciences, Franklin Lakes, USA), as well as the secondary antibody goat-anti-rabbit Alexa 568 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... the cells were tagged using a Sample Multiplexing Kit from BD. Biosciences ...