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Citations for Becton, Dickinson and Company :
1 - 50 of 326 citations for Fetal + Enhanced fetal bovine serum Aseptically Filled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 10% Bovine Fetal Serum (BD Difco, MD). Bacterial cells were washed twice in PBS and adjusted to 108 cells mL-1 in the biofilm broth and transferred to the 96-multiwell plates ...
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bioRxiv - Immunology 2020Quote: ... medium mixed with 10% fetal bovine serum (BD Biosciences, NY) for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... containing 10% fetal bovine serum (FBS) on type I collagen-coated flasks (BD Biosciences).
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bioRxiv - Systems Biology 2020Quote: ... Total EC (lin-CD31+) were sorted into 100% fetal bovine serum using FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl 100% fetal bovine serum and 16 μl of Matrigel (growth factor reduced; BD Biosciences) were then added to obtain the final mix ...
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bioRxiv - Cell Biology 2019Quote: ... strains were cultured in modified TYI-S-33 medium supplemented with bovine bile and 5% adult and 5% fetal bovine serum [56] in sterile 16 ml screw-capped disposable tubes (BD Falcon). Cultures were incubated upright at 37°C without shaking as previously described(Hagen et al. ...
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bioRxiv - Immunology 2019Quote: ... resuspended in 1% inactivated fetal bovine serum and analyzed by flow-cytometry with a LSRFortessaTM machine (BD Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 2% fetal-bovine serum in PBS and analyzed using the LSRFortessa flow cytometer (BD Biosciences). A total of 20,000 events were collected for each sample ...
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bioRxiv - Neuroscience 2021Quote: ... the cell pellet was suspended in alpha minimum essential medium (MEM) with 5% fetal bovine serum (FBS) and 0.6% glucose and filtered with a 40-μm cell strainer (BD Falcon). After centrifugation (200 × g ...
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bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The enzyme activity was stopped with fetal bovine serum (FBS) followed by the fixation and staining with the APC BrDU Flow Kit – Part A (552598, BD Pharmingen) according to the manufacturer’s recommendation with some exceptions ...
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bioRxiv - Developmental Biology 2019Quote: ... the trypsinised cells were resuspended in ice cold PBS + 10% Fetal bovine serum and subjected to FACS (BD Bioscience FACSAriaTM III). The GFP positive cells were sorted and collected in RP1 buffer and RNA was isolated using Nucleospin RNA XS kit (Macherey Nagel ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Immunology 2023Quote: ... Cells were plated in Tfh conditioning media (RPMI complete media with 10% fetal bovine serum, 10μg/ml anti-IL4 (BD Biosciences, clone 11B11), 10μg/ml anti-IFNγ (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10 % of heat-inactivated Fetal Calf Serum (FCS) (BD, Biosciences), 4 mM L-Glutamine (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 100 µg/ml streptomycin, and 10% fetal bovine serum before stimulation with 1X stimulation cocktail (eBiosciences, Waltham MA) with Golgi Plug (BD Biosciences, Franklin Lakes, NJ) before flow cytometry for 4-6 hours ...
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bioRxiv - Immunology 2024Quote: ... 100 µg/ml streptomycin, and 10% fetal bovine serum before stimulation with 1X stimulation cocktail (eBiosciences, Waltham MA) with Golgi Plug (BD Biosciences, Franklin Lakes, NJ) before flow cytometry for 4-6 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were diluted to a final concentration of 1 × 107 cells mL− 1 in staining buffer (fetal bovine serum) (BD Pharmingen, Franklin Lakes, NJ, USA); 2.5 µL of fluorescence-labeled antibodies was added to 50 µL of cell suspension ...
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bioRxiv - Physiology 2021Quote: ... cells were washed twice with FACS buffer (PBS plus 2% heat inactivated fetal bovine serum) and analyzed on a SORP 8 Laser BD LSR flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Genetics 2020Quote: ... Anti-Human Fetal Hemoglobin FITC 1:10 (BD Pharmigen 552829), and Anti-Hemoglobin B-(37-8 ...
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Feces were collected aseptically into anaerobic jars (BD Port-A-Cul™) and dissolved in anoxic PBS inside an anaerobic chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... fetal liver cells were stained with 1 μg anti-mouse Ter119-PE antibody (BD Pharmingen) per 106 cells for 20 minutes at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... MLN were collected aseptically and homogenized in BBL Mycoflask Thioglycollate (liquid) Prepared Media (BD Diagnostic Systems). Gifu medium agar plates containing the contents of the BBL Mycoflask Thioglycollate were incubated in an anaerobic incubator at 37°C for 48 h ...
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bioRxiv - Neuroscience 2023Quote: IL-17 concentration in serum was measured by cytometric bead array mouse IL-17A Enhanced Sensitivity Flex Set (BDBiosciences)24 or by electrochemiluminescence-based multi-array MSD V-Plex Mouse IL-17A Kit (MesoScale)88 ...
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bioRxiv - Microbiology 2022Quote: ... blood samples were collected aseptically and inoculated into BD™ BACTEC™ bottles at the bedside (BD™ BACTEC™ Plus Aerobic/F Culture vials Plastic [catalogue number 442023] for aerobic blood culture ...
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bioRxiv - Cell Biology 2024Quote: ... 30 mL of blood was aseptically collected into BD Vacutainer EDTA tubes (BD Biosciences, Mississauga, ON, Canada) and then gently layered on top of PolymorphPrep (Progen ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5 % bovine serum albumin) supplemented with CD133 antibody (5 % v/v, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and spleen were harvested and transferred aseptically to individual BBL Mycoflask Thioglycolate (Fluid) Prepared Media (BD Diagnostic Systems) and incubated for 72 h at 37°C ...
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bioRxiv - Genomics 2020Quote: ... with 0.1% bovine serum albumin (BSA) containing FITC-conjugated PAC-1 (BD Biosciences), PacBlue-conjugated CD42a (eBioscience ...
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bioRxiv - Microbiology 2019Quote: ... we filled separate 1-mL syringes (BD) with a solution of UltraPure distilled water (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... was filled with Matrigel© (BD Bioscience) and incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Sampling was performed aseptically with 3.5 mL syringes using a 0.8 mm x 50 mm Microlance needle (BD Biosciences). Microaerobic cultures were inoculated at an OD660nm of 0.1 from stationary-phase precultures in 50 mL Bio-One Cellstar Cellreactor tubes (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: Both strains were isolated by surface sterilizing infected Camponotus floridanus cadavers in 70% ethanol for 10 sec and aseptically removing ant cuticle with 25 G needles (PrecisionGlide, BD) to extract O ...
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bioRxiv - Microbiology 2021Quote: ... The tested bacterial colonies on each blood agar plate were aseptically transferred into Mueller-Hinton (MH) broth (BD Biosciences, America), and a homogenous suspension with a density equivalent to a 0.5 McFarland’s standard was prepared ...
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bioRxiv - Molecular Biology 2022Quote: ... SF was aspirated aseptically with a 19-gauge 40 mm needle and transferred to tubes containing ethylene diamine tetra acetic acid (EDTA) (BD Vacutainer ...
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bioRxiv - Cell Biology 2023Quote: ... The lungs were filled with a dispase (BD Biosciences)/agarose (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Animal caps were blocked for 1 hour using sheep serum and bovine serum albumin and then incubated in 1:250 anti-β-catenin antibody (BD Bioscience cat# 610154) in blocking solution overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... filled with 198 μL YNB media without ammonium sulfate (BD, Franklin Lakes ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Bovine Serum Albumin) followed by incubation with PE Mouse Anti-Human CD184 and CD44 antibodies (BD Biosciences Cat ...
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bioRxiv - Neuroscience 2019Quote: ... and enhanced green fluorescent protein cDNAs (eGFP; BD Bioscience) as described previously (Raingo et al. ...
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bioRxiv - Microbiology 2022Quote: ... a loopful from frozen glycerol stock will be aseptically transferred into 10 ml Tryptic Soy Broth plus 0.6% yeast extract (TSBYE) (Difco, Becton, Dickinson and Company, Sparks, Md.), (BeanTown Chemical) ...
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bioRxiv - Immunology 2023Quote: ... staining was performed on ice using the following panel of fluorochrome-conjugated antibodies diluted in stain buffer (0.5% BSA (Bovine Serum Albumin) supplemented with brilliant stain buffer plus (BD)) ...
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bioRxiv - Immunology 2021Quote: ... using a human IL-1β enhanced-sensitivity flex set (BD Biosciences). Undiluted cell supernatant was stained according to the manufacturer’s instructions and run on a BD FACS Canto II ...
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bioRxiv - Cancer Biology 2021Quote: ... γδ T cells were washed twice in cold FACS buffer (PBS, 5 mM EDTA, 1% bovine serum antigen) and stained with 1:20 anti-CD3-PerCP-Cy5.5 (BD Biosciences), 1:20 anti-TCRγδ-PE (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...