Labshake search
Citations for Becton, Dickinson and Company :
1 - 50 of 7315 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... TALON metal affinity resin (BD Bioscience), GFP-Trap magnetic agarose beads (Chromotek ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Binding (ACE2+/FLAG+) and non-binding (ACE2-/FLAG+) cells were sorted by FACS (BD FACSAria Fusion or Sony MA800 cytometer ...
-
bioRxiv - Biochemistry 2022Quote: ... incubated for 1-hour rocking at 4°C with a BV421 anti-flag antibody (BD Bioscience), washed twice with FACS buffers ...
-
bioRxiv - Molecular Biology 2019Quote: ... 50 µl cobalt beads (Talon metal affinity beads; BD Bioscience) that had been pre-blocked with BSA for two hours (by incubation of beads in 400 µl 1X PBS containing 100 µg BSA ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Binding (ACE2+/FLAG+) and non-binding (ACE2-/FLAG+) yeast cells were sorted by FACS (BD FACSAria Fusion). Collected cells were immediately checked for purity and resorted if necessary.
-
bioRxiv - Plant Biology 2023Quote: ... and the supernatant was mixed with TALON cobalt affinity resin (BD Biosciences) and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... The binding of GITRL or OX40L was detected using anti-FLAG APC antibody and measured on Accuri C6 cytometer (BD Bioscience). The data were analyzed using FlowJo software (BD Bioscience).
-
bioRxiv - Immunology 2019Quote: ... The expressed protein was purified by Talon metal affinity chromatography (BD Biosciences, CA) following the description ...
-
bioRxiv - Cell Biology 2022Quote: ... and low-affinity nerve growth factor receptor (NGFR, also known as p75; BD Pharmingen) for fluorescence-activated cell sorting (FACS) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Yeast cells expressing RBD that maintained antibody-binding (IgG+/FLAG+) or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or BD Influx). Collected cells were cultured in SD-UT medium for 16-48 hours at 30°C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Cells expressing RBD that maintained antibody-binding (IgG+/FLAG+) or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or Sony MA800 instrument). Collected cells were cultured in SD-UT medium for 16-40 hours at 30 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 750 μgs of protein were incubated with 25 μL of TALON Metal Affinity Bead suspension (BD Biosciences) in a 500 μL final volume for 30 min at 4°C with slow rotation ...
-
bioRxiv - Bioengineering 2023Quote: ... Each collection flag was swabbed using a polyester-tipped swab (BD BBL, 263000) and was resuspended in 200 μL nuclease-free water (Wang ...
-
bioRxiv - Physiology 2019Quote: ... Cells were then washed and resuspended in FACS buffer and analyzed for lectin binding affinity by performing flow cytometry analysis on an LSR II equipped with FACS DiVA software (version 6.1.1; BD). Measurement in the absence of lectins was performed as a negative control and at least 1 × 104 cells were analyzed for each sample ...
-
bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: scFv expressing (FLAG+) cells in the 4D5 library were sorted by FACS (BD Aria Fusion) first into HER2 binding and non-binding fractions ...
-
bioRxiv - Bioengineering 2023Quote: ... scFv expressing (FLAG+) cells in the 5A12 library were sorted by FACS (BD Aria Fusion) into High binding (VEGF+ or Ang2+ ...
-
bioRxiv - Bioengineering 2023Quote: ... Each collection flag was swabbed using a wet polyester-tipped swab (263000, BD BBL, USA) and was resuspended in 200 μL nuclease-free water ...
-
bioRxiv - Cell Biology 2021Quote: Expression of the various GPR116 constructs tagged with V5 or FLAG was verified by immunocytochemistry (Cytofix/Cytoperm, BD 51-2091KZ). Cells were plated in 8-chamber slides (Ibidi ...
-
bioRxiv - Biophysics 2022Quote: ... Fully melted 1.5% agarose gel (214010, BD, Franklin Lakes, New Jersey, USA) was poured onto the TomoDish and coverslipped ...
-
bioRxiv - Immunology 2020Quote: ... The serum was separated from the blood by serum gel tubes (BD) and incubated with Protein G high-capacity agarose beads (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... A second swab for microbiologic culture (BD BBL CultureSwab Plus Amies Gel) was then inserted into the posterior vaginal fornix immediately following collection of the first swab ...
-
bioRxiv - Pathology 2022Quote: Mouse blood was collected into a microtainer serum separator gel tube (BD Microtainer #365967 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or BD Influx). Collected cells were cultured in SD-UT medium for 16-48 hours at 30°C ...
-
bioRxiv - Biophysics 2019Quote: ... First-generation plasmodia were grown from sporae on 1% agar gel (Granulated; BD) using 150×15–mm culture plates (BD ...
-
bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech) into XPack CMV constructs (System Biosciences) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Collagen gels were prepared by combining 90μl of 3.68 mg/ml collagen (BD Biosciences), 10μl of 10X DMEM (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... blood was drawn into Vacutainer Gold Top 5 mL Silica Gel tubes (BD, #365968). Blood was allowed to clot for 30 min ...
-
bioRxiv - Systems Biology 2023Quote: ... The gel-purified amplicons were fused with the activation (AD) and binding domain (BD) Y2H expression vectors (pGADT7 and pGEO_BD ...
-
bioRxiv - Genomics 2020Quote: ... and a panel of 5 FITC-conjugated lineage antibodies (anti-CD41, anti-CD45R, anti-CD3e, anti-CD11b and anti-Ly-6G/6C, all at 1 μg/ml; BD 553848 ...
-
bioRxiv - Developmental Biology 2021Quote: ... endocardial cushions from the AV cushion were explanted on rat tail collagen gel (BD Biosciences). After an overnight incubation ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-CD63 and anti-GM130 (BD biosciences cat ...
-
bioRxiv - Immunology 2020Quote: ... rat anti-mouse anti-CD43-FITC (BD Pharmingen™ ...
-
bioRxiv - Immunology 2020Quote: ... hamster anti-mouse anti-CD3-FITC (BD Pharmingen™ ...
-
bioRxiv - Synthetic Biology 2019Quote: ... anti-CD8 (eBioscience) and anti-NKp46 (BD). To measure T cell production of cytokines ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-CXCR4 or anti-CD184 (BD Biosciences), 1:500 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CAFs were trypsinized and then seeded into 2mg/ml collagen gels (Rat tail collagen, BD Biosciences) at a density of 1×105 cells/gel in 24 well plates ...
-
bioRxiv - Microbiology 2019Quote: ... while blood collected via heart puncture utilized a polymer gel-based separator tube (BD Microtainer SST). Following collection ...
-
bioRxiv - Immunology 2021Quote: ... PE-CF594-conjugated anti-CD122 and BUV395-conjugated anti-CD244.2 were added along with anti-CD27 and anti-CD127 (BD Biosciences) as previously described.
-
bioRxiv - Immunology 2020Quote: ... rat anti-mouse anti-IgM-PE-Cy7 (BD Pharmingen™ ...
-
bioRxiv - Immunology 2020Quote: ... rat anti-mouse anti-CD45R/B220-APC (BD Pharmingen™ ...
-
bioRxiv - Immunology 2020Quote: ... anti-CD107a and anti-CD107b antibodies (BD Bioscience). Intracellular cytokine staining for CD107 ...
-
bioRxiv - Immunology 2022Quote: ... and anti-Ly6G and anti-Ly6C (BD Biosciences). Dead cells were excluded using Ghost Dye (eBioscience) ...
-
bioRxiv - Immunology 2020Quote: ... anti-Fas/CD95 and anti-IgA (BD Biosciences) for 20 min at 4 °C in PBS/FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... anti-CD14 and anti-CD235a purchased from BD Biosciences ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were stained with fluorochrome-conjugated antibodies (anti-CD15-V450-#642922, anti-CD24-PE-Cy7 #561646, anti-CD44-PE #550989, anti-CD184-PE-#555974; BD Biosciences) for 30 minutes on ice ...
-
bioRxiv - Cancer Biology 2020Quote: ... anti-hCD3 and/or anti-hCD44 (all from BD) antibodies to determine the fraction of viable human blasts (hCD3-hCD45+mCD45.1−hCD33+/hCD44+AnnV-cells ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-GM130 (610822) and anti-GS28 (611184) from BD Transduction Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-GM130 (610822) and anti-GS28 (611184) from BD Transduction Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... anti-IdU (mouse monoclonal anti-BrdU, BD Biosciences, 347580) and anti-ssDNA (mouse monoclonal IgG2a ...