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Citations for Becton, Dickinson and Company :
1651 - 1700 of 2194 citations for Recombinant Human dipeptidyl peptidase 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Immunology 2021Quote: ... and viability dye (eBioscience™ Fixable Viability Dye eFluor™ 780) followed by Foxp3 staining with anti-FoxP3 (259D/C7) using Human FoxP3 buffer set (BD biosciences) according to the Manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... and co-cultured with human CD8+ T cells at 1:1 E:T ratio in the presence of anti-CD107a-APC mAb (BD Biosciences, CA, USA). After 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: The purity of each NK population was checked before and after cell sorting and routinely by flow cytometry with anti-human CD16-BV421 (clone 3G8, BD Pharmagen, France), anti-human CD56-PeCy7 (clone B159 ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Myelin-depleted single-cell suspensions obtained from high grade glioma samples were sequentially labeled using the BD Human Sample Multiplexing Kit (Sample-tag) (BD Biosciences, #633781), the sorting antibody CD45-APC (#560973 ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody concentrations were as follows: alveolar macrophage membranes were stained using CD206 PE-CF594 (Mouse Anti-Human CD206, BD Biosciences; catalogue number: 564063) at a concentration of 4µL in a 50μL final staining volume per section ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 45 min with the indicated antibodies: AlexaFluor® 647 anti-human CD107a antibody (diluted at 1/100, clone H4A3; BD Pharmingen TM), anti-human Perforin (10µg/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 50 µL Perm/Wash buffer containing a cocktail of anti-human antibodies including IFNγ BV480 (BD, 566100, clone B27), TNFα BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Human platelets in the obtained blood were identified using APC-labeled polyclonal rabbit anti-human (h) CD41 and/or CD42b (BD Pharmingen, 1:100) and fluorescein isothiocyanate (FITC)-labeled polyclonal rabbit anti-mouse (m ...
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bioRxiv - Bioengineering 2024Quote: ... and stained at room temperature for 1 hour using a PE labeled mouse anti-IDO1 antibody (12-9477-42, Thermo, Waltham, MA) or a BV786 labeled mouse anti-human HLA-DR antibody (564041, BD, Franklin Lakes, NJ) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Cancer Biology 2021Quote: Activated OTI T-cells were combined with MC38-OVA tumor cells at a 1:4 effector:target ratio and cultured together with 0.6μL/mL GolgiStop (BD Biosciences) for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... For intracellular staining the cells were incubated for 4 h with the protein transport inhibitor GolgiPlug (BD Biosciences). For fixation and permeabilization of the cells ...
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bioRxiv - Cell Biology 2020Quote: ... slides were briefly rinsed with PBS and stained overnight at 4°C with antibodies (mouse anti-EPS8, BD Transduction Laboratories Cat#610144 ...
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bioRxiv - Microbiology 2022Quote: ... Strains were grown and maintained at 32 °C on Casitone-yeast extract (CYE) (1% casitone, 0.5% yeast extract, 10 mM MOPS [pH 7.5], 4 mM MgSO4) 1.5% agar (BD Difco) plates or in CYE liquid medium at 32 °C on a rotary shaker at 220 rpm.
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bioRxiv - Biochemistry 2022Quote: ... The retinas were then incubated overnight at 4℃ with a monoclonal VE-cadherin antibody (1:100, BD Biosciences) and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for immunoblot were as follows: AURKA (1:1000; mouse mAb clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C [1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200, BD Biosciences, Cat. N: 561664), anti-Eomes-PE (1:200 ...
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bioRxiv - Immunology 2019Quote: ... Sections were stained at 4°C overnight with primary rat anti-mouse CD11b-FITC (M1/80; BD Bioscience) and rat anti-mouse Ly6G-FITC (1A8 ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed in 600μl of 4% PFA and acquired on an LSR II Flow Cytometer (BD Biosciences). Compensation was run and applied for each experimental replicate and voltages consistent throughout ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Samples were run on a Fortessa Flow Cytometer equipped with 4 lasers and using DiVa software (BD Biosciences). Compensation and analysis was performed using FlowJo software (Tree Star ...
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bioRxiv - Microbiology 2021Quote: ... cells were resuspended in PBS containing 4% paraformaldehyde (PFA) and were analysed on a BD LSRFortessa (BD Biosciences). Gating strategies are shown in S8 and S9 Figs ...
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bioRxiv - Cancer Biology 2021Quote: ... KYSE-150 cells were loaded with 5 µM Fluo-4 in 96-well imaging plates (BD Falcon, NJ) at room temperature for 20 min ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Genetics 2022Quote: ... Diluted segregant cultures were grown for 4 hours prior to sorting on a FACSAria II cell sorter (BD). Plots of side scatter (SSC ...
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bioRxiv - Bioengineering 2022Quote: Cells were incubated with fluorochrome-labeled antibodies for 30 minutes at 4°C in binding buffer (BD Biosciences), prior to analysis using a BD Accuri™-B6 flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... cells were fixed and permeabilised in 100μL Cytofix/Cytoperm solution for 20 minutes at 4°C (BD Biosciences). Cells were then washed twice in Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mice were intraperitoneally injected with 1 ml of 4% Brewer’s modified thioglycolate medium Brewer Modified (BD company, USA). Four days later ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was incubated overnight at 4°C with mouse anti-LR11 (1:500, #612633, BD Transduction Laboratories) for SORLA detection ...