Labshake search
Citations for Becton, Dickinson and Company :
1401 - 1450 of 1608 citations for 6 BENZOFURAN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
-
bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
-
bioRxiv - Immunology 2022Quote: ... Then live Tetr-gag+Pent-gag+CD3+CD8+ cells were sorted by flow cytometry into PBS buffer 1% BSA 2 mM EDTA using a FACSAria III (BD Biosciences) equipped with 488 ...
-
bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
-
bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
-
bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
-
bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...
-
bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
-
bioRxiv - Microbiology 2024Quote: ... pyogenes M1T1 5448 strain (56) were grown aerobically at 37°C in Todd-Hewitt Broth supplemented with 2% yeast extract (THY, BD, France). Liquid and solid cultures of L ...
-
bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
-
bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
-
bioRxiv - Immunology 2024Quote: ... Colonies were then picked for shape and hemolytic activity and cultured in Todd Hewitt Broth supplemented with 2% Yeast Extract (Fisher 50489152) or Brain Heart infusion media (BD DF0037178) in 5%CO2 at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
-
bioRxiv - Immunology 2023Quote: ... 1 μg/ml each of ESAT-6 and CFP-10 peptide pools (provided by Aeras, Rockville, MD) or R10 media for 2 h before adding 10 μg/ml BD GolgiPlug (BD Biosciences) for a further 12 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell pellets were dissolved in 2% FBS in PBS and flow cytometry was performed using an Accuri C6 Plus (BD Biosciences) or Citole Flow Cytometer (Beckman Coulter).
-
bioRxiv - Immunology 2024Quote: ... Stimulated and unstimulated cells were harvested and washed with 2 mL of FACS buffer and then cells were fixed with BD transcription factor fixation/perm buffer (cat #562574, BD Bioscience) for 20 minutes at room temperature ...
-
bioRxiv - Pathology 2023Quote: ... Placentas were perfused for 3 hours and 1 ml (T4 transfer experiments) or 2 ml (TRIAC transfer experiments) samples were collected from the reservoirs into blood collection tubes (BD vacutainer) at the time points indicated in the figures ...
-
bioRxiv - Immunology 2022Quote: ... The cells were fixed in 2% paraformaldehyde and GFP expression measured and quantitated using a FACS Canto II flow cytometer and FlowJo software (BD Biosciences). IC50 values were calculated using Prism software (GraphPad/Dotmatics).
-
bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
-
bioRxiv - Immunology 2023Quote: ... Cells were sorted by their distinct proliferation peaks (peak 0, peak 1 and peak 2) on FACS-Aria and Influx Cell Sorters (BD Biosciences). Cells were kept on ice and after washing with cold PBS were immediately resuspended in Trizol for RNA extraction ...
-
bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
-
bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
-
bioRxiv - Cancer Biology 2023Quote: ... were stained with the specified antibodies on ice for 30 min in D-PBS supplemented with 2% FBS in the dark and analyzed using a Fortessa or Symphony A3 (BD Biosciences). Data was analyzed using FlowJo10 (Tree Star Inc. ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed with PBS three times and resuspended in FACS buffer (PBS with 2%FBS) for analysis on BD Biosciences LSR Fortessa using a 488-nm laser and 530-nm/30 filter (BD Biosciences). Data analysis was performed with FCS Express 7 (De Novo Software) ...
-
bioRxiv - Developmental Biology 2023Quote: ... resuspended in 300 μl of 2% FBS in PBS with 1000 ng/μl DAPI and processed by FACS on a Melody Cell Sorter (BD Biosciences) using the BD FACSChorus software ...
-
bioRxiv - Developmental Biology 2023Quote: ... GFP and mCherry+ cells were collected in 50 µl 2% BSA in PBS via fluorescence-activated cell sorting (BD LSR Fortessa) and processed for single-cell RNA sequencing analysis based on 10X Genomics (10X Chromium system ...
-
bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
-
bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
-
bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
-
bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
-
bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
-
bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
-
bioRxiv - Microbiology 2023Quote: ... GYM (glucose 4 g, yeast extract 4 g, malt extract 10 g, CaCO3 2 g, and Bacto Agar (BD Difco™) 20 g ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... and the second model investigated ii) four exposures from the first eating event (AK-20, BD, KB, LT & NH; Model 2). In each model ...
-
bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
-
bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
-
bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... or FAK-KD cell lines were generated by injecting a total of 2×106 MDA-MB-231/Dendra2 cells resuspended in 20% collagen I (BD Biosciences) in PBS into the lower left mammary gland of SCID mice ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were suspended in 350 µL of FACS buffer (1XDPBS, 2% FBS, 25mM HEPES, 2mM EDTA) and was analysed by flow cytometry BD LSRFortessa (BD Biosciences). 10,000 cells were analysed for each condition ...
-
bioRxiv - Cell Biology 2023Quote: ... with a purity >95% into sterile filtered PBS with 2% FBS or acquired on a FACS Canto II or LSR fortes Analyzer (BD Biosciences). Single cells were index sorted using single cell depositor ...
-
bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...