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Citations for Becton, Dickinson and Company :
951 - 1000 of 2149 citations for Recombinant Human CD3E His and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Single cell suspensions stained in PBS + 2% FBS in the presence of Fc receptor blocking antibody (BD Pharmingen) and stained with the antibodies against the following mouse proteins ...
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bioRxiv - Microbiology 2023Quote: ... and Fixable Viability Dye eFluor 780 (Thermo Fischer) in presence of FC Block (BD, 20 min, 4°C). Stained cells were washed in FACS buffer twice and fixed in 2% paraformaldehyde (30 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were Fc-blocked with anti-mouse CD16/CD32 (1:100, Cat# 553141, BD Biosciences, San Jose, CA) followed by surface marker staining with antibodies to MerTK (2B10C42 ...
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bioRxiv - Immunology 2023Quote: ... and incubated for another 15 minutes at 4°C in the presence of Fc receptor block (BD Biosciences). Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences, Franklin Lakes, NJ). For RPE and endothelial identification ...
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bioRxiv - Cell Biology 2023Quote: FC analyses of SkM single cell suspensions were performed with the cell analyser LSRFortessa X-20 (BD Bioscience) using the FACSDiva 8.0 software ...
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bioRxiv - Immunology 2024Quote: ... 2mM EDTA) and incubated with Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 (BD Biosciences) to block non-specific staining ...
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bioRxiv - Immunology 2024Quote: The FCS files of CyTOF were analyzed with dimensionality reduction and clustering approaches using FlowJo-10 (BD Biosciences) and its plugins including DownSampleV3 ...
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bioRxiv - Immunology 2021Quote: ... PBMCs and mdDCs culture supernatants were quantified by the Cytometric Bead Array (CBA) method using the Human Inflammatory Cytokines Kit and Human Chemokine Kit (BD Biosciences), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5µl/10 million cells ...
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bioRxiv - Immunology 2020Quote: ... Flow cytometry experiments were performed using PE-anti-human CD54 (ICAM-1) (REA266, Miltenyi) and APC-anti-human CD20 (clone L27, BD Biosciences). Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58 ...
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bioRxiv - Microbiology 2021Quote: ... Purification of human CD34+ cells were assessed by flow cytometry using an anti-human CD34-FITC antibody (clone 581, BD Biosciences). Fetal liver derived from three different donors were used in this study.
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bioRxiv - Immunology 2022Quote: Suspensions of human Tconv cells or Treg cells were stained with V500-conjugated anti-human CD4 (clone RPA-T4, BD Biosciences), PE-Cy7-conjugated anti-human CD25 (clone BC96 ...
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bioRxiv - Genetics 2020Quote: ... and permeabilized using BD Cytofix/Cytoperm kit before incubation with BV605 anti-human IFN-gamma (B27 clone) and BUV395 anti-human TNF-alfa (MAB11 clone, BD Biosciences) in permeabilization buffer at 4°C for 30 min in the dark ...
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bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5μL/10 million cells ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines created with fluorescently tagged proteins were sorted and normalized for expression using the UC Berkeley Flow Cytometry Facility (BD Bioscience Influx Sorter). Cell lines were confirmed with confocal imaging and immunoblot.
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bioRxiv - Immunology 2020Quote: ... Human cytokines expression (hTNFα and hIFNγ) in human T cells were analyzed using BD LSRFortessa™ cell analyzer - flow cytometer (BD Biosciences) and the data were analyzed using FlowJo software (Dako).
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were from BD Pharmingen and Santa Cruz Biotechnology Inc. ...
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bioRxiv - Immunology 2022Quote: Flow cytometry of peritoneal cells was performed using anti-mouse CD16/32 (Fc Block, BD Biosciences, San Jose, CA) before staining with primary antibody or isotype controls ...
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bioRxiv - Microbiology 2021Quote: ... 1×106 cells were blocked with mouse BD FC block™ (5 μg/mL, BD Pharmingen, Cat No. 553141) and then stained using fluorescent-labeled monoclonal antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... for 15 minutes to block Fc receptors and then stained using with antibodies to CD45-APC-Cy7 (BD 557659) and SYTOX Red Dead Cell Stain (Life Technologies S34859 ...
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bioRxiv - Immunology 2020Quote: ... cells were washed in perm/wash solution and resuspended in 0.5% PFA-FC stain buffer prior to running on a Symphony A5 flow cytometer (BD). Analysis was performed using FlowJo software ...
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bioRxiv - Systems Biology 2022Quote: Citrated blood collected from infected and control mice was diluted with HEPES buffer containing mouse Fc-block (BD Pharmingen). Platelets ...
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bioRxiv - Immunology 2019Quote: ... FCS in PBS and were stained for 1 hour at 37°C with Vγ5 TCR-FITC (clone 536; BD), MHCII-AF647 (I-A/IE ...
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bioRxiv - Immunology 2020Quote: ... and 10% (v/v) FCS (from herein referred to as DC culture media. The antibodies were purchased from BD, Miltenyi ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:100, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed and blocked with blocking buffer plus Fc receptor blocking antibody (Clone Fc1.3216, BD Biosciences, United Kingdom). Cells were incubated at RT for 10 min then centrifuged (800 g ...
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bioRxiv - Immunology 2022Quote: Cells were incubated with purified anti-mouse CD16/CD32 clone 2.4G2 (Fc block; BD Biosciences, San Jose, CA, USA), and stained as described with H-2k(d ...
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bioRxiv - Immunology 2024Quote: ... 1% FCS and 2mM EDTA) and stained for extracellular molecules with the following antibodies: anti-CD8a (53-6.7, BD), anti-CD4 (GK1.4 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences) and stained with cell surface marker detecting fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... and blocking with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). Intracellular proteins were stained with fluorophore-conjugated antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and Fc-receptors were blocked (2.4G2) before staining with monoclonal antibodies before collection on a Fortessa flow cytometer (BD). Fluorochrome-conjugated antibodies to the following cell surface molecules were used ...
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bioRxiv - Immunology 2024Quote: ... cells were blocked with 2% FBS and 0.2% goat serum containing HBSS including 1:1000 Mouse BD Fc Block™ (Clone 2.4G2, BDBiosciences). For detection of endogenous p65 ...
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bioRxiv - Cell Biology 2023Quote: ... Data collection was done using BD FACSDiva™ software and .fcs files were formally analyzed with FlowJo (FlowJo, BD). All flow cytometry experiments contained single color controls for compensation and gating.
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bioRxiv - Neuroscience 2023Quote: ... Fc receptors were blocked for 15 min with rat anti-CD16/32 (1:200, catalog no. 553141; BD Biosciences) and cells were washed and labeled with the following antibodies for 30 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... All data were converted to FCS 3.0 using NF Profession (Version 1.0, NanoFCM) and analyzed with FlowJo (Version 10.8.1, BD, Franklin Lakes, NJ).
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bioRxiv - Immunology 2023Quote: ... Non-specific antibody binding was blocked by incubating the cells with Fc-blocking antibody 2.4G2 (BD, 25 μg/ml) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed in PBS+2% FCS and permeabilised for 10 minutes in 70ul of Cytofix/Cytoperm (BD Biosciences). After washing in 2mL of PermWash ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in FACS buffer and incubated in FC Block for 5 minutes (1:60, BD Pharmingen, 553142) and primary antibodies for 10 minutes ...
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bioRxiv - Pathology 2023Quote: ... single cells were sorted into an eppendorf containing PBS with 5% FCS using the FACSAria III sorter (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: ... Fc receptor blockade and viability staining were simultaneously performed using Rat anti-mouse FcψRIII/II (CD16/32; BD Biosciences) and ZombieNIR (BioLegend ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from tumors or spleens were incubated in Fc block (BD Biosciences, 553142, vol/vol 1/50) at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... and blocked unspecific bindings with using anti-mouse CD16/CD32 (Mouse BD Fc Block, BD Biosciences, Franklin Lakes, NJ). Cells were then stained with FITC-conjugated anti-mouse CD45 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-human CD4-PE-Cy7 (SK3, BD Biosciences) and mean fluorescence intensities (MFIs ...
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bioRxiv - Cancer Biology 2021Quote: ... PE-CF594 Mouse Anti-Human CD68 (Cat564944; BD Horizon), APC-H7 Mouse Anti-Human HLA-DR (Cat561358 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-mouse/human CD44-APC (clone !M7, BD Biosciences), and DAPI ...