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Citations for Becton, Dickinson and Company :
801 - 850 of 4520 citations for Recombinant Mouse Tnfrsf13c Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... Unspecific binding of antibodies was blocked by previous incubation for 10 min with anti CD16/CD32 (Fc block, clone 2.4G2; #55314, BD Pharmingen) in Fluorescence Activated Cell Sorting (FACS ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and incubated (15-20 min., on ice) with Fc Block (1:100 in 1% FBS/PBS, Clone 2.4G2, BD Biosciences), followed by anti-CD45-PE-Cy5.5 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Pellets were suspended in 100 μl of flow buffer and blocked with 0.25 μg Fc block (BD Pharmingen, CA, USA) for 10 min followed by staining with antibodies ...
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bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: Cell pellets were eluted in FACs buffer (0.5% BSA in PBS with 2mM EDTA) and blocked for ten min with Fc preblock (CD16/CD32, BD 553141), followed by 20 minute staining with anti-CD31-APC (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in PBS with 1% FCS and analyzed for GFP and AF647 fluorescence on a FACS Verse (BD). Nontransfected and non-AF647-stained cells were used for gating the cells positive for GFP and AF647 ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked with 3% (w/v) bovine serum albumin (BSA, ChemCruz) in PBS with 0.5 g/mL Fc Block (BD Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... All samples were then incubated with Fc block (5 μg mL-1 CD16/CD32 (BD Biosciences, San Diego, CA, USA) and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then treated with dasatinib for 1 hr at 37°C prior to blocking with Fc-block (BD Bioscience), 5 % normal mouse serum ...
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bioRxiv - Immunology 2021Quote: ... Surface antibody cocktail was prepared in cell staining buffer (Fluidigm) and added to all samples equally after Fc block (BD). Cells were washed then fixed and permeabilized using eBioscience Transcription Factor Fix/Perm Buffer followed by barcoding (Fluidigm) ...
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bioRxiv - Genomics 2021Quote: ... Cell counting was performed by counting of the events with FCS ≥ 1.104 and SSC ≥ 5.105 by flow cytometry (BD-Accuri-C6). The transposition activity was represented as the ratio of the hygromycine-resistant colonies to the total cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... Fc blocking was performed with a combination of antibodies diluted in FACS buffer with Brilliant Violet Stain Buffer (BD Biosciences) for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... the stained cells were stored in DPBS supplemented with 2.5% (v/v) paraformaldehyde (PFA) and 1% FCS before flow cytometry measurement on a BD LSRFortessa flow cytometer (BD Biosciences). pMHC-I multimer binding T cells were identified by a Boolean gating strategy in FlowJo (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted macrophage fraction was resuspended in a final volume of 200μl MACS buffer and incubated 10 min with Fc block (1:50, BD Pharmingen). 200μl of staining solution containing flow cytometry antibodies (anti-CD45-PECy7 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Immunology 2024Quote: Enriched B cells were resuspended in ice cold FACs buffer at a density of 106 cells/100 μL and treated with Human Fc Block (BD) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding of IGSF3-Fc to the cell surface was detected as the APC fluorescence intensity using FACSVerse (BD Biosciences).
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bioRxiv - Systems Biology 2023Quote: All flow cytometry raw data were saved as .fcs files and processed by the FlowJo software (version 10, BD Biosciences) for further analysis ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse anti-human CD63 and mouse IgG isotype control were purchased from BD Biosciences (San Jose ...
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bioRxiv - Cancer Biology 2024Quote: ... PE rat anti-mouse IgM and BV605 rat anti-mouse IgD (BD Pharmingen). First ...
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bioRxiv - Developmental Biology 2019Quote: ... Interactions with desalted analytes diluted in PBS at 100 nM (recombinant CBP domains 6His-tag Full HAT, Bromodomain (BD), RING) ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-mouse gp91phox monoclonal antibody (1:50, clone 53, BD Biosciences, UK) overnight at 4°C in PBS with 1% FCS and 0.05% Triton X-100 ...
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bioRxiv - Cell Biology 2021Quote: ... Commercial primary antibodies were mouse anti-GM130 and mouse anti-p230 (BD Transduction Labs), and rabbit anti-giantin (Covance Research Products) ...
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bioRxiv - Immunology 2021Quote: ... mouse sera were incubated with biotin rat anti-mouse IgG1 (BD Pharmingen, Cat. 550331) or biotin rat anti-mouse IgG2a (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-mouse gp91phox monoclonal antibody (1:50, clone 53, BD Biosciences, UK) or rabbit anti-mouse NFκB monoclonal antibody (1:400 ...
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bioRxiv - Cancer Biology 2024Quote: ... primary antibodies for mouse anti-human CD49b (611017) mouse anti-EEA1 (610457) from BD Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... PE rat anti-mouse CD62E (for E-selectin, BD Biosciences 553751, 10µg per mouse), Alexa594 anti-mouse Ly-6G/Ly-6C (Gr-1 ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy7 or AF647-conjugated mouse anti-mouse CD64 (X54-5/7.1; BD Biosciences), AF700-conjugated mouse anti-mouse CD45.2 (104 ...
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bioRxiv - Cell Biology 2024Quote: ... mouse IgG1 (#555746; 1:100) and mouse IgG2 (#555740; 1:100) all from BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then resuspended in 200 uL FACS buffer and transferred to 96 well U bottom plates for staining with Fc block (BD Biosciences), zombie fixable viability stain for 20 min ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Immunology 2019Quote: ... 10 ml PBS with 10 % FCS (buffer A) was added and the suspension was passed through a 100 µm cell strainer (BD Biosciences). The resulting single cell suspension was pelleted and resuspended in 1 ml ACK buffer (0.15 M NH4Cl ...
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bioRxiv - Immunology 2019Quote: ... and CD3+CD4+CD25− cell population (Tconv) were directly sorted into tubes with 500 μL FCS on a FACSAria™ III (BD). For RNA sequencing ...