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Citations for Becton, Dickinson and Company :
801 - 850 of 896 citations for 58K Golgi protein Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Proteins were detected using anti-HA (Covance cat# MMS-101P, 1:1000) and anti-Actin (BD cat# 612657, 1:1000) antibodies ...
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bioRxiv - Immunology 2020Quote: ... The levels of IFNg protein expression was determined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
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bioRxiv - Immunology 2020Quote: ... IFN-γ and IL-4 protein in culture supernatants were quantified using the Cytokine Bead Array according to the manufacturer’s instructions (BD Biosciences)
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
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bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-Lhx1 (1:250, gift from Dr. Masanori Taira) and mouse anti-E-cadherin (1:100, BD Transduction Laboratories, 610182). For detection of primary antibodies the following secondary antibodies were used ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Neuroscience 2020Quote: ... dissected tissues were then incubated overnight with rabbit anti-Afg3l2 (1:250, Antibody plus CSB-PA04005A0Rb) and mouse anti-cox4 (1:1000, BD Biosciences) antisera ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cell were washed three times with HF buffer and incubated with secondary antibodies APC-Cy7 goat anti-rabbit IgG (BD, 611203) used at 1:200 dilution and AF647 Donkey anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: anti-histone H3 (Ser10) (rabbit polyclonal; Cell Signal #9701) and anti-E-cadherin (mouse monoclonal, clone 36; BD Biosciences). The following secondary antibodies were use ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with rabbit anti-GRA7 (1:5000) (gift from John Boothroyd, Stanford) and rat anti-LAMP1 (1D4B, BD Biosciences) (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... washed with PBS-T and kept overnight in 1% BSA solution in PBS-T containing a 1:500 dilution of anti-rabbit-Vi-polysaccharide antibody (BD BioSciences) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg ...
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bioRxiv - Cell Biology 2020Quote: ... and macrophage chemoattractant protein-1 (MCP-1) were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) in accordance with the instructions of the manufacturer.
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Cell Biology 2019Quote: ... macrophage inflammatory protein (MIP-1α) and tumor necrosis factor (TNF-α) were detected using Cytometric Bead Array (CBA) technology (BD Biosciences) and assayed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: Cytometric bead array (CBA) was performed using the mouse soluble protein master buffer kit combined with the appropriate CBA flex sets (BD Biosciences), per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 1:50 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences) with side-scatter (height ...
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bioRxiv - Cell Biology 2022Quote: ... Green fluorescent protein (GFP) positive cells in HRasL61 with GFP transfected cells were sorted using flow-cytometry (FACS Aria II; BD Bioscience).
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bioRxiv - Physiology 2022Quote: The geometric mean fluorescence intensities (MFI) corresponding to the probes/target protein levels were determined by flow cytometry acquired on Aria II (BD Biosciences) or CytoFLEX (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 344SQ murine lung adenocarcinoma cells expressing firefly luciferase and green fluorescent protein (in 50 μL of 1:1 mix of HBSS and BD Matrigel) were injected into the left lung of 8 weeks old female 129/Sv mice via intrapulmonary injection as described previously (50) ...
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Cell Biology 2021Quote: ... and monoclonal cell lines were generated by isolating cells expressing both TauRD fusion proteins in a 96 well-plate by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Protein transport inhibitor containing Monensin and protein transport inhibitor containing Brefeldin A were added to cells (both 10 μg/ml, BD Biosciences) and cells cultured for an additional 4 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected with 5 mg/mL puromycin and sorted based on red fluorescent protein (RFP) by fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...