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Citations for NanoTemper :
101 - 150 of 153 citations for Cyclic GMP Direct ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 5% glycerol with 0.1% Tween20 was labelled with Monolith His-Tag Labelling Kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified recombinant PTPN2 protein (20 μM) was labeled according to the protein labeling kit RED-NHS (Nanotemper, Cat. No. L001). The K73 compound was diluted into 16 concentration gradients with 1xPBS-P using the fold-and-half dilution method ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µL of 100 nM solution of his-tagged protein was labeled with RED-tris-NTA 2nd Generation dye (Monolith HIS-tag labeling Kit, NanoTemper). Protein-protein complexes were obtained by mixing dye-labeled IFIT protein (final concentration 50 nM ...
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bioRxiv - Immunology 2022Quote: ... Germany) and MST-Red-MIF was prepared using the Monolith Protein Labeling Kit RED-NHS 2nd Generation from NanoTemper (Munich, Germany), following the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RBD was labeled with the RED-NHS 2nd generation Monolith Protein Labeling Kit according to the manufacturer’s instructions (Nanotemper; MO-L011). The fluorescent group was conjugated to RBD through primary amines ...
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bioRxiv - Microbiology 2019Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Labeling was performed by incubating PlaF with 15 μL dye (440 μM stock solution) for 2.5 h using the NHS labeling Kit (NanoTemper, Munich, Germany). PlaF was then transferred into a purification buffer using PD-10 columns ...
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bioRxiv - Biochemistry 2021Quote: 6xHis-eIF1 was labeled with a Cy5 fluorophore through non-covalent linkage to the His-tag moiety using the Monolith Protein Labeling Kit RED-tris-NTA 2nd generation (NanoTemper Technologies). 40S subunits (0.58 nM –1.2 μM ...
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bioRxiv - Microbiology 2022Quote: ... were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd generation according to the recommendations of the manufacturer (NanoTemper Technologies). Briefly ...
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies, Germany). After labeling ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The TACC3 domain was fluorescently labeled via NHS coupling using the standard NHS Red labeling kit following the manufacturers protocol (Nanotemper, Germany). MST experiments were recorded on a Nanotemper Monolith instrument using a concentration of the fluorescently labeled TACC domain of 600 nM while the concentration of the H5 peptide was varied in the range of 0–400 µM ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018, NanoTemper Technologies, GmbH, Munich, Germany) per the manufacturer’s instructions with slight modifications (43) ...
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bioRxiv - Cancer Biology 2019Quote: BRCA1 tBRCT wild type or mutant domains used in the assay were labeled with NT-647-NHS fluorescent dye using the Monolith NT™ Protein Labeling Kit (NanoTemper Technologies). Assays were carried out in 20mM Tris buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... with increments of 1 °C per minute using a Prometheus NT.48 instrument (NanoTemper Technologies).
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent NbSyt1 was incubated with different dilutions of purified Syt-1(97-421) using Premium Coated Capillaries (NanoTemper). For operation of the instrument and evaluation of affinity data ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: Purified proteins after SEC were concentrated to 0.5-1 mg/mL for NanoDSF using a Prometheus NT.48 (Nanotemper). Capillaries were filled up with ∼ 15 µL of protein for melting scans between 20-95 °C with a pre- stabilization phase of 1 min and a temperature slope of 2 °C/min (37 min ...
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bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... was incubated with the different peptides for 15 minutes and subsequently heated from 20°C to 95°C at 1°C/min using a Nanotemper Prometheus NT.48 fluorimeter (Nanotemper) controlled by the PR.ThermControl software (version 2.1.2) ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Microbiology 2019Quote: ... The binding affinities were determined to fit the data points to a quadratic equation (equation 1) using non-linear regression using MO Affinity software (NanoTemper Technologies), Prism (GraphPad Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... fluorescently labeled protein and ligand were mixed in 1:1 volume and then incubated for 5 minutes at room temperature before loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies). MST was measured using a Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... 10 ⌈L of 1 mg/mL ADCs were loaded into standard capillaries and mounted in a Prometheus NT.48 (Nanotemper Technologies, Germany) with excitation near UV ...