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Citations for EpiCypher :
1 - 23 of 23 citations for Recombinant Human HDAC8 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Plant Biology 2024Quote: ... recombinant mononucleosomes (EpiCypher) as substrates ...
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bioRxiv - Molecular Biology 2023Quote: One μg of recombinant (Epicypher) or cellular nucleosomes (see protein purification above ...
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bioRxiv - Biochemistry 2021Quote: ... the arrays were incubated with a 1:1000 dilution of anti-GST (EpiCypher, 13-0022) in 1% milk 1x PBST for two hours at 4oC with shaking ...
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bioRxiv - Biochemistry 2024Quote: ... One ug of recombinant designer demethylated nucleosomes (EpiCypher) was incubated with the PHF21A-containing complexes from 293T cells ...
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bioRxiv - Cell Biology 2021Quote: Recombinant semi-synthetic designer nucleosomes (dNucs) were from EpiCypher. Two phases of dCypher® testing on the PerkinElmer AlphaScreen® platform were performed as previously described9 ...
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bioRxiv - Cell Biology 2019Quote: ... 15 uL of recombinant nucleosomes (SNAP-ChIP, EpiCypher, 19-1001) were fixed with fresh 1% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...