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Citations for Avidity :
1 - 50 of 139 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... coli.19 The purified proteins were biotinylated using biotin protein ligase (Avidity); excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity, #BirA500). Biotinylation of the NiV protein probes was confirmed by biolayer interferometry by testing the ability of the biotinylated protein to bind to streptavidin sensors ...
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bioRxiv - Immunology 2020Quote: ... purified PR8-HA and S proteins were biotinylated using BirA biotin-protein ligase (Avidity). Biotinylated PR8-HA and S proteins were fluorescently labelled by the sequential addition of streptavidin-conjugated to phycoerythrin (PE ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... biotinylated (BirA biotin-protein ligase bulk reaction kit [Avidity, USA]) and purified by size exclusion chromatography (Superdex 75 column [GE Healthcare] ...
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bioRxiv - Immunology 2020Quote: ... biotinylated (BirA biotin-protein ligase bulk reaction kit [Avidity, USA]) and purified by size exclusion chromatography (Superdex 75 column [GE Healthcare] ...
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bioRxiv - Immunology 2021Quote: ... biotinylated (BirA biotin-protein ligase bulk reaction kit [Avidity, USA]) and purified by size exclusion chromatography (Superdex 75 column [GE Healthcare] ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.325 μg/μl Maltose Binding Protein (MBP)-AviTag substrate (Avidity, L.L.C.), 8.3 mM ATP and 42 μM biotin ...
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bioRxiv - Immunology 2024Quote: ... The purified spike protein was biotinylated using the BirA biotin-protein ligase reaction kit (Avidity) following the manufacturer’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation was performed using a BirA biotin-protein ligation kit (Avidity) on proteins produced with a C-terminal avidin tag sequence ...
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bioRxiv - Microbiology 2020Quote: ... a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA) was used to biotinylate protein samples ...
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bioRxiv - Immunology 2020Quote: ... The RBD protein was biotinylated with the BirA biotin-protein ligase kit (Avidity LLC, Colorado, USA) using the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... BirA biotinylation of S proteins was conducted using BirA biotin-protein ligase bulk reaction kit (Avidity). Coronavirus S proteins with Avi-tags were concentrated to 7-9 mg/ml using 100K Amicon tubes in TBS ...
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bioRxiv - Immunology 2021Quote: Recombinant HA trimers were biotinylated by addition of biotin–protein ligase (Avidity). To generate HA tetramers ...
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bioRxiv - Microbiology 2020Quote: ... S proteins with Avi-tag were pre-biotinylated using BirA biotin-protein ligase standard reaction kit (Avidity). 25 nM S-614D or 15 nM S-614G in 10X kinetic buffer (ForteBio ...
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bioRxiv - Bioengineering 2021Quote: ... S1 protein was biotinylated at the AVI-tag using the BirA biotin-protein ligase kit (Avidity Biosciences) and premixed at 5 nM with serial dilutions of VNAR-hFc antibodies for 1 hr at 4°C ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 Wuhan-Hu-1 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before18 ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 Wuhan-Hu-1 RBD and NTD were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before (11) ...
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bioRxiv - Immunology 2022Quote: ... AviTagged tri-S and RBD proteins were biotinylated using BirA biotin-protein ligase bulk reaction kit (Avidity, LLC) or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... purified spike ectodomain trimers and RBD domain protein were biotinylated by the addition of biotin-protein ligase (Avidity). Biotinylated proteins were buffer exchanged into PBS ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation of monomer was carried out using BirA biotin-protein ligase kit (Avidity) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Overnight biotinylation reactions were performed using the BirA Biotin-Protein Ligase Kit (Avidity) at 4℃ in 1x BiomixA ...
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bioRxiv - Immunology 2020Quote: ... purified S1B/RBD or S proteins were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Biotinylated-CoV proteins were captured on streptavidin coated 96-well plates (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the purified RBDs were biotinylated using the BirA biotin-protein ligase reaction kit (Avidity). The biotinylated proteins were re-purified and concentrated as described above ...
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bioRxiv - Immunology 2021Quote: ... each FcγR was biotinylated using a BirA biotin-protein ligase bulk reaction kit (Avidity) according to the protocol of the manufacturer ...
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bioRxiv - Immunology 2023Quote: BSP-tagged proteins were biotinylated using the BirA biotin-ligase bulk reaction kit (Avidity), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... biotinylation with BirA biotin-protein ligase standard reaction kit (Avidity, 318 LLC-Aurora, Colorado), and purification using size-exclusion column (Waters ...
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bioRxiv - Immunology 2023Quote: ... rhesus macaque FcγR2A and FcγR3A (acquired from the Duke Human Vaccine Institute Protein Production Facility) were biotinylated with a BirA biotin–protein ligase bulk reaction kit (Avidity). C1Q (Sigma ...
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bioRxiv - Immunology 2022Quote: Aliquots of RBD-HISAVI protein were biotinylated in vitro using the BirA500:BirA biotin-protein ligase standard reaction kit (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... VH F6 was biotinylated with BirA biotin-protein ligase standard reaction kit (BirA500, Avidity, USA). Streptavidin biosensors (ForteBio ...
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bioRxiv - Immunology 2020Quote: ... purified spike ectodomain trimers were biotinylated by addition of biotin-protein ligase (Avidity, Aurora, CO). Biotinylated spike ectodomain trimers were buffer exchanged into PBS ...
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bioRxiv - Immunology 2023Quote: ... 500μM biotin (Avidity) was then added at a 1:100 ratio relative to the total solution volume and incubated rotating for 10min at RT ...
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bioRxiv - Microbiology 2021Quote: ... TGEV and CCoV-HuPn-2018 were biotinylated using BirA biotin-protein ligase standard reaction kit (Avidity) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Biophysics 2024Quote: ... and subsequently biotinylated at the C-termini Avi tag sequence via BirA biotin-protein ligase (Avidity) (31) ...
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bioRxiv - Microbiology 2021Quote: ... The samples were then biotinylated in vivo using a BirA biotin-protein ligase standard reaction kit (Avidity). Following the kit instructions ...
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bioRxiv - Immunology 2020Quote: All antigens with an Avi tag were biotinylated enzymatically using BirA biotin-protein ligase (Avidity, Bulk BirA) while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: Purified mini-HA was biotinylated using the Biotin-Protein Ligase-BIRA kit according to the manufacturer’s instructions (Avidity). Biotinylated mini-HA was then conjugated to streptavidin– PE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... avi-tagged proteins were biotinylated with a BirA500 biotin-ligase reaction kit according to the manufacturer’s instruction (Avidity). TT was purchased from Creative Biolabs (Vcar-Lsx003) ...
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bioRxiv - Immunology 2023Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Free biotin (Avidity, Cat. #BIO200) was added to the beads at 5 μM and incubated for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Free biotin (Avidity, Cat. #BIO200) was added to the beads at 5 μM and incubated for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD or SARS-CoV-2 RBD KEN mutant (K417N, E484K, N501Y) was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before 3 ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD or SARS-CoV-2 RBD K417N/E484K/N501Y mutant was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before3 ...
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bioRxiv - Immunology 2020Quote: ... Folded MHC-I molecules were biotinylated using the BirA biotin-protein ligase standard reaction kit (Avidity, LLC-Aurora, Colorado), and MHC-I monomers were purified using size exclusion chromatography (HPLC ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 NTD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before (Robbiani et al. ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylation was performed using the BirA biotin-protein ligase standard reaction kit (BirA-500) as per the manufacturer’s instructions (Avidity). This BG505 trimer probe was further purified by SEC on a HiLoad 16/600 Superdex 200 PG column (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before 1 ...