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Citations for KeraFAST :
1 - 50 of 64 citations for SARS Coronavirus Membrane Protein Matrix E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-Matrix Protein (Kerafast, Inc.; EMS009) or goat anti-Influenza A Virus (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... while the lower portion of the membrane was probed with anti-VSV matrix protein antibody (Kerafast, EB0011; loading control) diluted 1:2,500 in 5% skim milk solution ...
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bioRxiv - Microbiology 2019Quote: ... IAV matrix protein monoclonal antibody (E10; catalog no. EMS009, Kerafast) at 1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... anti-matrix protein [E10] (M1 and M2, 1:1,000, Kerafast cat. no. EMS009), and anti-SARS-CoV-2 spike protein (RBD ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was evaluated as FFU/mL using the anti-VSV-Matrix protein 23H12 antibody (Kerafast Inc.) to detect infected cells.
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... coli LOBSTR (Kerafast) cells following cold shock and induction with 0.5 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Kerafast) were transformed with the expression vector and grown at 37 °C in LB medium with 0.4% (w/v ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli LOBSTR (Kerafast) cells and plated on LB ampicillin (100 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... coli cells (Kerafast) and Get1 was expressed in E ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Immunology 2019Quote: ... anti-Zika E antibody (Kerafast) was used ...
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bioRxiv - Cell Biology 2023Quote: ... coli LOBSTR cells (Kerafast), and grown in 2XYT media supplemented with 10 μM ZnCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... coli LOBSTR strain (Kerafast) in TB medium supplemented with kanamycin (50 µg/mL ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Molecular Biology 2021Quote: ... coli LOBSTR expression strain (Kerafast). Cells were grown overnight at 18°C in TB medium and expression was induced with 0.05 mM of IPTG at OD260nm = 0.8-1.0 ...
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bioRxiv - Microbiology 2021Quote: ... coli LOBSTR-BL21(DE3) (Kerafast) containing pET29b-DpaA-his (encoding PelB-DpaA(20-246)-His6 ...
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bioRxiv - Biochemistry 2021Quote: ... coli LOBSTR cells (EC1001, KeraFast) were used for the expression of PRAS40 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression strains (Kerafast, EC1002) were transformed ...
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bioRxiv - Microbiology 2024Quote: ... coli LOBSTR strain (Kerafast EC1002). Cell pellets from 50 ml E ...
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bioRxiv - Microbiology 2021Quote: ... and anti-VSV matrix (VSV-M) (23H12, 1:1,000; Kerafast Inc.) antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... coli LOBSTR-RIL(DE3)53 (Kerafast) cells and protein production was induced with 0.2 mM IPTG at 18 °C for 12-14 h ...
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bioRxiv - Microbiology 2021Quote: ... coli Lobstr BL21 (DE3) RIL (Kerafast), and protein was produced in the M9 media ...
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bioRxiv - Biophysics 2019Quote: Escherichia coli LOBSTR-RIL(DE3) (Kerafast)43 cells were co-transformed with vectors ...
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bioRxiv - Biochemistry 2019Quote: ... coli strain LOBSTR(DE3) RIL (Kerafast) 46 was transformed with the MBP-TorsinA construct ...
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bioRxiv - Microbiology 2021Quote: ... coli LOBSTR-BL21(DE3) cells (Kerafast) harbouring the pET28a-LdtE-his plasmid were grown in 2 L of LB-autoinduction medium (LB supplemented with 0.5% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC-CBM ...
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bioRxiv - Biochemistry 2022Quote: ... coli expression strain LOBSTR (Kerafast, #EC1001) to minimize purification of endogenous Ni(II ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LOBSTR cells (Kerafast) to generate the various NEC oligomeric and heterodimeric interface mutants (listed in Supplementary Table S18) ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to produce the NEC-SUPUL31 and NEC-DNUL34/SUPUL31 crystallization constructs ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220-His8 ...
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bioRxiv - Biochemistry 2019Quote: ... coli BL21 (DE3) (Kerafast Inc, Boston, MA) cells transformed with DDX21 expressing pET28b ...
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bioRxiv - Biochemistry 2021Quote: ... coli LOBSTR-BL21 (DE3) RIL (Kerafast Inc) using a pET26b-derived expression vector (81) ...
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bioRxiv - Biochemistry 2022Quote: ... coli (Kerafast, Winston-Salem, NC, Ref: EC1002) transformed via heat shock with plasmids validated by sequencing ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) LOBSTR cells (Kerafast EC1002) were employed for recombinant protein expression and purification ...
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bioRxiv - Bioengineering 2022Quote: ... coli strains DH5α and LOBSTR-BL21(DE3) (Kerafast, #EC1002). NikABCDE was cloned into the first site ...
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bioRxiv - Cell Biology 2021Quote: ... coli expression strain LOBSTR (Andersen et al., 2013) (Kerafast, Boston, MA) transformed with pTf16 ...
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Biochemistry 2020Quote: The Nup84-Nup133CTD vector was transformed into Escherichia coli LOBSTR-RIL(DE3) (Kerafast)68 cells and protein production was induced with 0.2 mM IPTG at 18 °C for 12-14 h ...
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bioRxiv - Microbiology 2020Quote: ... L and G proteins (Kerafast), all of which under T7 promoter control ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (Bigalke et al ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (NEC construct absent of the UL34 C-terminal transmembrane helix)14 ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was expressed in LOBSTR (Kerafast) E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio ...
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bioRxiv - Neuroscience 2019Quote: ... The membranes were probed with anti-puromycin antibody (KeraFAST, Cat # EQ0001, 1:1000). The relative amount of loading was determined by β-actin ...
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bioRxiv - Immunology 2022Quote: ... Membranes were probed overnight at 4°C with anti-RNA:DNA hybrid (S9.6) (Kerafast) at 1:10,000 dilution or dsDNA antibody (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Blocked membrane was incubated overnight with antibody against R-loops (Clone S9.6, Kerafast) under rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... eEF3 protein was detected with anti-eEF3 antibody (Kerafast ED7003) and Glucose-6-phosphate dehydrogenase (G-6-PDH ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-MLV transmembrane protein/p15E (clone 42/114, Kerafast), rabbit anti-myc (Cell Signaling Technology) ...