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Citations for Cell Biolabs :
1 - 50 of 55 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
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bioRxiv - Neuroscience 2020Quote: ... Type 2 serotypes of rAAV vectors were prepared using the AAV Helper Free Packaging System (Cell Biolabs, San Diego, CA, USA). The serotypes were produced in HEK 293 cells using a helper virus-free system and were purified using two CsCl2 density gradients and titrated by quantitative polymerase chain reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Viruses were produced with Serotype 1 or DJ (Cell Biolabs, Inc.) and purified by CsCl gradients 67 ...
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bioRxiv - Bioengineering 2021Quote: ... Each AAV serotype was packaged separately using a triple transfection method32using 293AAV cells (Cell Biolabs). AAVs were purified by iodixanol gradient ultracentrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... pHelper and pAAV-RC (serotype 9 or DJ; purchased from Cell Biolabs Inc., San Diego, CA) using a standard calcium phosphate method ...
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bioRxiv - Neuroscience 2019Quote: ... pHelper and pAAV-RC (serotype 9 or DJ; purchased from Cell Biolabs Inc., San Diego, CA, USA) plasmid using a standard calcium phosphate method ...
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bioRxiv - Cancer Biology 2022Quote: ... along with the envelope plasmid pCMV-VSV-G (Cell Biolabs, San Diego, CA, USA), into cells Plat-GP packaging cells (Cell Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... and the virus titer was measured based on the titer of the viral protein p24 with QuickTiterTM Lentivirus Titer Kit (Cell Biolabs) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... at 70% confluency were transfected with a mix of Eco envelope plasmid (Cell Biolabs, 320026), pCMVR8.74 (Addgene ...
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bioRxiv - Neuroscience 2019Quote: Mycoplasma-free HEK293-AAV cells (Cell Biolabs Inc., Cat. # AAV-100) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... AAVs (DJ serotype) were generated in HEK293T cells with the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc., cat# VPK-400-DJ) using the manufacturer-suggested protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with the pAAV-hSyn-OsTIR1-P2A-mAID-EGFP and two AAV helper plasmids encoding serotype DJ (Cell Biolabs, San Diego, CA, USA) using polyethylenimine (Polysciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus expressing sgRNAs were packaged and produced using HEK293T cells with pCMV-VSV-G envelope vector (Cell Biolabs, Cat. # RV-110) and pCMV-dR8.91 packaging vector (Addgene ...
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bioRxiv - Immunology 2023Quote: ... Virus was titered using the QuickTiter™ Lentivirus Titer Kit (Cell Biolabs, Inc) following manufacturer conditions.
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bioRxiv - Neuroscience 2020Quote: AAV virus was made using the AAV-DJ Helper Free Packaging System (Cell Biolabs) using published protocols95 ...
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bioRxiv - Neuroscience 2021Quote: Adeno-associated virus (AAV) was generated using the AAV-DJ Helper Free system (Cell Biolabs) as published previously9,27 ...
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bioRxiv - Cell Biology 2022Quote: ... For production of virus for mouse cells platinum E cells (Cell Biolabs, Cat#RV-101) were transfected ...
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bioRxiv - Neuroscience 2019Quote: Adeno-associated virus (AAV) vectors were packaged using the AAV Helper Free Expression System (Cell Biolabs) as described previously45 ...
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bioRxiv - Cell Biology 2021Quote: ... Virus titer was measured using the QuickTiter™ Lentivirus Titer Kit (Cell Biolabs, VPK-107-T) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The virus packaging cell lines Platinum-E (Plat-E,Cell Biolabs, Inc, San Diego, CA, USA) and GPp210-GFP61 were kept in DMEM (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid expressing the VSV-G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The lentiviral plasmid used to generate the HEK-293T stable cell lines expressing the human ACE2 gene (GenBank ID NM_021804 ...
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bioRxiv - Biochemistry 2021Quote: ... while the plasmid expressing VSV G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The HDM-IDTSpike-fixK plasmid was employed as a template for site-directed mutagenesis to generate the expression plasmid for the D614G mutant of SARS-CoV-2 spike protein ...
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bioRxiv - Neuroscience 2021Quote: Fibcd1 constructs were packaged into virus by CaCl2 transfecting 70-80% confluent 10cm2 plate of Platinum-E (PlatE) HEK 293T cells (Cell Biolabs #RV-101) with 10µg Fibcd1 construct and 2µg gag/pol plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... and co-transfected with pAAV-DJ vector and pAAV-Helper vector into HEK 293T cells to generate recombinant adeno-associated virus expressing Mir20b or anti-Mir20b according to the manufacturer’s protocol (Cell Biolabs, San Diego, CA). The AAVs were purified with AAVpro® Purification Kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: Protein carbonyls were visualized using the OxiSelect Protein Carbonyl Immunoblot Kit (Cell Biolabs). Tnrf1-/- BMDMs were seeded in 24-well plates at 2.5x105 one day prior to infection with indicated L ...
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bioRxiv - Microbiology 2023Quote: Protein carbonyls were quantified using the OxiSelect Protein Carbonyl ELISA Kit (Cell Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Protein carbonylation was measured using the OxiSelect Protein Carbonyl ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein oxidation levels were quantified using an OxiSelect Protein Carbonyl ELISA Kit (Cell Biolabs, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: Protein carbonyl levels in heart tissues were measured using OxiSelect protein carbonyl ELISA kit (Cell Biolabs, San Diego, CA) according to the manufacture’s instructions.
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bioRxiv - Physiology 2022Quote: ... Duplicate gels were run to assess protein carbonyls using a kit which derivatises proteins directly on the membrane following transblotting (Cell Biolabs). For this ...
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bioRxiv - Physiology 2021Quote: ... Protein carbonyls were measured by following the protocol in the commercially available OxiSelect Protein Carbonyl Immunoblot Kit (Cell Biolabs STA-308) as previously performed (Konopka et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Levels of protein carbonyls [a stable marker of reactive oxygen species (ROS)-induced oxidation] in ALF (using 8 μg of protein) was determined using OxiSelect Protein Carbonyl ELISA Kit (Cell Biolabs, Inc.). Levels of 3-nitrotyrosine-containing proteins [a stable marker of reactive nitrogen species [RNS]-induced oxidation ...
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bioRxiv - Neuroscience 2019Quote: ... pAAV2/2 (Cell Biolabs Inc., Cat# VPK-422), pAAV2/5 (Cell Biolabs Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA, Cell Biolabs) were used for mitochondrial and intracellular ROS staining ...
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bioRxiv - Neuroscience 2021Quote: ... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The concentration of oxidized proteins in plasma samples was quantified using the OxiSelect Advanced Oxidation Protein Products (AOPP) Assay Kit (Cell Biolabs, Inc, Cat. No. STA-318). Plasma samples were diluted 1:2 in assay buffer prior to performing the assay according to manufacturer instructions with modifications ...
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bioRxiv - Immunology 2022Quote: Protein lysates were generated using RIPA buffer containing protease inhibitor (Cell Biolabs) on ice for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: MDA-MB-231 breast cancer cell line (triple negative) expressing red fluorescent protein (RFP) (Cell Biolabs) were cultured in DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: Protein carbonylation was measured using the OxiSelect 8-OHdG Oxidative DNA Damage ELISA Kit (Cell Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: mCherry protein levels were detected with the aid of RFP ELISA kit (Cell Biolabs, Inc., USA). All protein samples were diluted to equal concentration prior to loading on antibody plate ...
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bioRxiv - Cancer Biology 2023Quote: Cell adhesion to ECM proteins was performed using a CytoSelect 48-well Cell Adhesion Assay Kit (Cell Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... and a marker of protein nitration (3-nitrotyrosine) were measured in sea turtle plasma using ELISA kits (Cell Biolabs catalog numbers ...
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bioRxiv - Cell Biology 2021Quote: ROS levels in kidney tissues were assessed by 4-Hydroxynonenal (4-HNE) protein adduct levels measured using the OxiSelect™ HNE Adduct Competitive ELISA kit (STA838, Cell Biolabs) according to the manufacturer’s instruction.
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bioRxiv - Physiology 2021Quote: ... We assayed plasma 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels (OxiSelect™ Oxidative DNA Damage Elisa kit, Cell Biolabs Inc.) and insuline-like growth-factor 1 (IGF-1 ...
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bioRxiv - Immunology 2019Quote: LDL/VLDL was isolated from 2-3ml pooled healthy male or female serum using LDL/VLDL Purification Kit (Ultracentrifugation Free) (CELL BIOLABS) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Differentiated brown fat DE 2.3 cells were washed with 2 mL PBS and processed according to the manufacturer’s protocol (Cell Biolab # MET-5071) to measure the intracellular taurine concentrations.
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bioRxiv - Cancer Biology 2023Quote: The wound healing assay SVIP siRNA-transfected and negative siRNA transfected MCF7 and T47D cells were plated at 2 ×105 cells per well into Cytoselect wound-healing assay 24 well plate (Cell Biolab, Inc). Each well contained an insert that created a wound field ...
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bioRxiv - Immunology 2021Quote: Physical particles were determined by measuring p24 levels using the QuickTitre™ Lentivirus Titre which quantifies lentivirus-associated HIV rather than free p24 proteins (Cell Biolabs – VPK-107-T). Manufacturer’s protocol was followed ...
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bioRxiv - Plant Biology 2022Quote: ... Ten micrograms of total protein were separated on 10% SDS- PAGE gel and were followed by protein blot analysis using mouse anti- methylglyoxal monoclonal antibodies (Cell Biolabs, Catalog number- STA-011).