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Citations for Cell Biolabs :
1 - 43 of 43 citations for Adenovirus Type 5 Particles CMV Luciferase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and adenovirus was concentrated by ViraBind™ adenovirus purification kit (Cell Biolabs, VPK-100). Adenovirus titer was measured by counting GFP-positive cells 24 h after infection with serial dilution ...
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bioRxiv - Bioengineering 2023Quote: ... and QuickTiter™ Adenovirus Titer ELISA Kit (Cell Biolabs, San Diego, CA), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... AAV-CMV-YFP-FKBP and AAV-CMV-Tom20-CFP-FRB were produced based on AAV-DJ CMV Expression System (Cell Biolabs, VPK-410-DJ). HEK293T cells were seeded in two six-well cell culture plates (FALCON ...
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bioRxiv - Neuroscience 2019Quote: ... The QuickTiter Adenovirus Titer Elisa kit was purchased from Cell Biolabs (San Diego, CA). Antisedan ...
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bioRxiv - Neuroscience 2021Quote: ... AAV2-CMV-LacZ (Cell Biolabs, Catalog number AAV-342), AAV1/2-EF1a-N-CretrcintG (Cat #B387Harvard Medical School Gene Vector Core) ...
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bioRxiv - Neuroscience 2020Quote: ... FLAG-Drp1S637A and GFP from the CMV promoter were as previously described (Filippi et al, 2017) using the RAPAd CMV Adenoviral Expression system (Cell Biolabs, Inc. San Diego, CA, USA.), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The scAAV-CMV-GFP plasmid was purchased from Cell Biolabs (Cat. No. AAV410). The insulin-like growth factor 1 (IGF1 ...
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bioRxiv - Cancer Biology 2021Quote: SKOV3-luciferase (Cell Biolabs, San Diego, CA) were grown in RPMI containing 10% FBS and 0.8 mg/ml neomycin ...
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bioRxiv - Immunology 2021Quote: ... The titres were determined on T-REx™ cells using anti-hexon immunostaining assay based on the QuickTiter™ Adenovirus Titer Immunoassay kit (Cell Biolabs Inc).
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bioRxiv - Cell Biology 2020Quote: ... immortalized brown preadipocytes were infected with ecotropic lentiviral particles (Cell Biolabs, Inc.) with a scrambled (Scr) ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral particles were created using the ViraSafe lentiviral packaging system (Cell Biolabs). ShRNA oligo sequences were based upon the Sigma-Aldrich MISSION shRNA library and were obtained from Integrated DNA technologies ...
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bioRxiv - Bioengineering 2019Quote: ... was subcloned downstream of the ubiquitous CMV promoter into the AAV-MCS vector (Cell Biolabs Inc).
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bioRxiv - Neuroscience 2019Quote: ... Retroviral particles were generated by transfection of PLAT-E cells (Cell Biolabs, Inc.) with the retroviral vector encoding MC4R-3×HA using Lipofectamine 3000 (Thermo fisher scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... After purification, it was cloned into pAdtrack-CMV (Quantum Biotechnologies, Inc.) or pAAV-MCS (Cell Biolabs, Inc.) plasmids under the control of a CMV or a CAG promoter respectively ...
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bioRxiv - Neuroscience 2020Quote: The luciferase assay with HEK 293 cells (293AAV cell, Cell Biolabs) was performed as described previously (Tsuboi et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Viral particles were purified using Virabind AAV Purification Kit (Cell Biolabs, Inc., cat# VPK-140). AAV titers were approximately 1.0 ×1011 infectious units/ml ...
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bioRxiv - Immunology 2023Quote: Homology donors comprising sequences to be inserted and flanking homology arms were cloned into plasmid ITR-CMV-GFP (containing ITRs from AAV2; Cell Biolabs) or pVAX by Infusion cloning (Takara) ...
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bioRxiv - Neuroscience 2019Quote: ... the U6-VDAC1-shRNA sequences were then cut using ApaI and BstEI to be cloned into a pAAV-CMV-GFP vector (Cell Biolabs, Inc.), the pAAV-mito-GCaMP2 ...
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bioRxiv - Physiology 2021Quote: ... The AAV-CMV-GFP plasmid was developed by inserting a CMV promoter and GFP (ZsGreen1) into a promoterless AAV vector (Cat. No. VPK-411-DJ; Cell BioLabs, USA) using In-Fusion Cloning (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... under control of the CMV promoter were constructed and produced using AAV-DJ Helper Free Expression System and 292AAV cell line (Cell Biolabs, Inc.). The viral tier was determined by standard qPCR with the BioRad SYBR Green supermix using serial dilutions of the cloning vector pAAV-MCS as standards.
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bioRxiv - Immunology 2021Quote: ... Lentivirus was packaged into VSV-G pseudotyped particles using the third-generation ViraSafe Lentiviral Packaging System (Cell Biolabs) and Lenti-Pac 293Ta cells (GeneCopoeia)(24) ...
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bioRxiv - Bioengineering 2021Quote: ... The number of lentiviral particles were quantified using the QuikTiter Lentivirus Quantitation Kit from Cell Biolabs (HIV P24 ELISA). All reported viral titers were within the linear range of the standard curve made using a positive control ...
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bioRxiv - Genomics 2021Quote: Constructs based on the RT3REN backbone (see Supplementary Table 4) were packaged into lentiviral particles using the Platinum-E packaging cell line (Cell Biolabs). In brief ...
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bioRxiv - Cancer Biology 2023Quote: The firefly luciferase cDNA was cloned into the PSMPUW-Hygro lentiviral vector (Cell Biolabs, catalog number: VPK-214). Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were verified to be free of residual viral particles 10 days post transduction using the QuickTiter™ Lentivirus-Associated HIV p24 titer kit (Cell BioLabs Inc.).
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bioRxiv - Immunology 2022Quote: ... coli particles conjugated with HRP (horseradish peroxidase) in 96-well plates per manufacturer’s protocol (CytoSelect 96-well phagocytosis assay, Cell Biolabs, San Diego CA) for 3 hours in 37°C incubator with 5% CO2.
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bioRxiv - Cell Biology 2021Quote: ... 5 × 106 Plat-A cells (Cell Biolabs) were plated on a 10 cm plate in media without antibiotics ...
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bioRxiv - Neuroscience 2020Quote: ... Type 2 serotypes of rAAV vectors were prepared using the AAV Helper Free Packaging System (Cell Biolabs, San Diego, CA, USA). The serotypes were produced in HEK 293 cells using a helper virus-free system and were purified using two CsCl2 density gradients and titrated by quantitative polymerase chain reaction ...
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bioRxiv - Neuroscience 2019Quote: ... pAAV2/5 (Cell Biolabs Inc., Cat# VPK-425), pAAV2/6 (Cell Biolabs Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... DCFH-DiOxyQ (STA-347-5, Cell Biolabs, USA). RPE/choroid complex was homogenized ...
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bioRxiv - Cancer Biology 2023Quote: ... by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs, catalog number: VPK-206) with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The full cDNA sequence of the lncRNA was then amplified with forward primer: 5’- GTATCATAAGGATCCCTTTCCACTGCTCTGGTGAG-3’ and reverse primer: 5’- GTATCATAAGTCGACCTCACCTAGCTGTCTGTCC-3’ and cloned into pAAV-MCS (Cat#: VPK-410, Cell Biolabs Inc.) using the restriction enzymes BamH I and HindIII sites ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 5 x 106 Platinum E cells (PE cells; Cell Biolabs) were plated in 10-cm dishes ...
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bioRxiv - Cell Biology 2023Quote: ... about 5 x 106 Platinum-E packaging cells (Cell Biolabs, Inc, San Diego, CA) were transfected with 5 μg of Flag_HsIRE1a_pBabePuro ...
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bioRxiv - Biochemistry 2019Quote: ... 10‐20 μg plasmid DNA and 5 μg helper plasmid (pCMV-Gag-Pol, Cell Biolabs) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Comet assays were performed using the OxiSelect Comet Assay Kit (Cell Biolabs Cat#STA-351-5) with alkaline (pH > 7.0 ...
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bioRxiv - Physiology 2022Quote: ... and subsequently incubated with primary antibody against DNP (Cell Biolab; 1:1000 dilution 5% FSG TBS-T) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Intracellular NO levels were measured using OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Cell Biolabs, STA-800-5). Nitrite levels in the cleared supernatant were measured using Griess Reagent Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... grown in 10 cm dish were transiently transfected with the following plasmids: 5 µg of pLenti-GFP (Cell Biolabs), 6 µg of psPAX2 and 0.9 µg of pCMV-VSVG (Cell Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then blocked for 1 hour at room temperature in blocking buffer containing 5% PhosphoBLOCKER blocking reagent (Cell Biolabs) in TBST ...
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bioRxiv - Synthetic Biology 2019Quote: ... AAV6-CCR5-PGK-GFP was produced at Sangamo Therapeutics as previously described.5 All other AAV vectors were produced in-house using the AAV helper free packaging system (Cell Biolabs). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 ×104 cells were seeded into 24-well plates containing CytoSelect™ 24-Well Wound Healing Assay inserts (Cell Biolabs, San Diego, CA, USA) (16) ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR fragment encoding ERp44 was cloned into the 5′ BamHI and 3′ XhoI sites of the pMXs-IRES-Blasticidin retroviral vector (RTV-016) (Cell Biolabs, Inc., San Diego, CA USA). The following primers were used to produce the C29S mutation of ERp44 using QuikChange mutagenesis ...