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Citations for Lucigen :
1 - 50 of 187 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Molecular Biology 2022Quote: ... For RNAse-free IP conditions TBS-0.05% NP-40 was supplemented with 1U/ul NxGen RNAse inhibitor (Lucigen). For nuclease treated samples ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were lysed with 4000U of RNase-free lysozyme (Lucigen) at 37 °C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... the total RNA was treated with RNase free DNase1 (Epicentre Biotechnologies, USA) by incubating at 37 °C for 15 min ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: Five micrograms of small RNAs was dissolved in nuclease-free water and incubated with TEX (Lucigen) for 1 h at 30°C according to the manufacturer’s protocol ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Neuroscience 2022Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... RNase R (Epicentre) treated for 1 h at 40°C and supplied with DNase I (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase R (Lucigen) digestion was performed to degrade linear RNA ...
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bioRxiv - Genetics 2022Quote: ... RNase Inhibitor (Lucigen) and NxGen T7 RNA polymerase (Lucigen ...
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bioRxiv - Genomics 2023Quote: ... RNase inhibitor (Lucigen) was added at 0.4 U/µl throughout the incubation until the rolling cycle amplification (RCA ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Genetics 2019Quote: ... An additional RNase step was performed using RiboShredder RNase Blend (Epicentre Biotechnologies). Three independent biological repeats were obtained for each sample ...
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bioRxiv - Neuroscience 2022Quote: RNAse R (Lucigen, #RNR07250) treatment was performed on 1 μg total RNA for 10 minutes at 37°C using 5U of enzyme ...
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bioRxiv - Physiology 2024Quote: ... RNase inhibitor (30281, Lucigen), Maxima H-RTase (EP0751 ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl RNase H buffer and 2 μl hybridase thermostable RNase H (Lucigen) preheated to 45° were added ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 18.75 U RNase R (Lucigen), 4.375 U RNase T (NEB) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
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bioRxiv - Genomics 2019Quote: ... 2.5 µl RNase Inhibitor (Lucigen), and 2.5 µl of 100 µM 10x_TSO ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), 1X NEB buffer 3.1 (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), and nuclease-free water (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase H (10U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 μl containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM KCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.25 units RNase I (Epicentre) was added to 5ul of sample in a total volume of 50 ul in IP buffer supplemented with 0.1% Igepal ...
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM LiCl ...
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bioRxiv - Immunology 2024Quote: ... for which RNase H (Epicentre) treatment was used ...
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bioRxiv - Genomics 2019Quote: ... we added 5μL preheated Hybridase RNase H reaction mix (10U Hybridase Thermostable RNase H [Lucigen H39500] ...
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bioRxiv - Microbiology 2023Quote: ... RNase-H master mix was prepared for each depletion (3µl Hybridase Thermostable RNase H; Epicentre), 0.5µl 1M Tris-HCl pH=7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 2 μl Maxima H- RTase (EP0751 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen) and 31 μl water ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U/ml Rnase Inhibitor (Lucigen), 2.5 μM Template Switch Oligo ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl RNase R mixture (Epicentre) was added to the sample before incubation at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... RNase H (10 U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 μl containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of RNase inhibitor (Lucigen) and 450 μl of IP buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 U NxGen RNase Inhibitor (Lucigen), and 40 U Superscript IV RT (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2U/uL RNAse inhibitor (Lucigen). The tissue was then triturated 15 times through a fire-polished Pasteur pipette and then passed through a 40 uM filter to remove cell debris and large chunks ...
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bioRxiv - Molecular Biology 2023Quote: ... After treatment with Rnase A (Epicentre) at 37°C for 20 minutes and Proteinase K (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µl RiboGuard RNase Inhibitor (Lucigen) in a total volume of 20 µl for 60 minutes at 30°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.6 u/μl NxGen RNase inhibitor (Lucigen) and 20.3 nM radiolabeled primer ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 u/µl NxGen RNase inhibitor (Lucigen) and 5% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl of RiboGuard RNase Inhibitor (Epicentre) was added to the eluted RNA to protect against ribonucleases ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
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bioRxiv - Cell Biology 2021Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...