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Citations for Lucigen :
1 - 50 of 750 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RNA was harvested from snap-frozen pellets (using liquid nitrogen) using the MasterPure Yeast RNA Purification Kit (Epicentre) and stored at −80 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were size-purified on TBE-Urea gels before being circularized by CircLigase II (Epicentre). Circularised cDNAs were then annealed to an oligonucleotide complementary to the BamHI site and then BamHI digested ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... from at least two freshly starved 9 cm plates of the appropriate worm strain using the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), following manufacturer protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from the frozen tissues (10 mg) with a MasterPure Complete DNA and RNA purification Kit (Lucigen). We used Q5®High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 serial dilution samples and a non-evolved yLL132a ancestor with the MasterPure Yeast DNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Crude genomic DNA was generated by incubating yeast isolates at 95°C in 50 μL of 20 mM NaOH for 10 minutes or the MasterPure™ Yeast DNA Purification Kit (Lucigen). PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution (corresponding to 10 µg of total RNA) was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 minutes at a temperature of 25°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 U Ampligase (Lucigen), and molecular-grade H2O were added to bring the total volume to 45 μl ...
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
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bioRxiv - Developmental Biology 2022Quote: ... RNase H (10 U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 μl containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted from one plate using QuickExtract DNA extraction solution (Epicentre) to perform genotyping by PCR using primer pairs specifically recognizing the mutated sequences introduced by the ssODN and a genomic sequence adjacent to the region of integration ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-U endonuclease (Lucigen Cat#DB0715K) for 30-min at 37°C and then incubated at 65°C for 10-min to stop the digestion ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 U/μL ReadyLyse lysozyme (Epicentre) and incubated for 1 h at RT ...
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bioRxiv - Bioengineering 2019Quote: ... 0.5 μL ampligase (10 U/μL, Lucigen), and 1 μL 10X ampligase buffer (Lucigen) ...
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bioRxiv - Genomics 2021Quote: ... 10 µL of RNase Inhibitor (Lucigen, #F83923) was added per 1 mL suspension immediately before microfluidic encapsulation ...
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bioRxiv - Biochemistry 2022Quote: ... coli 10 G chemically competent cells (Lucigen). Successful transformants were miniprepped and confirmed via Sanger sequencing (UW–Madison Biotechnology DNA Sequencing Core Facility) ...
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bioRxiv - Biochemistry 2022Quote: ... coli 10 G chemically competent cells (Lucigen). Successful transformants were miniprepped and confirmed via Sanger sequencing (UW–Madison Biotechnology DNA sequencing core facility) ...
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bioRxiv - Biochemistry 2022Quote: ... coli 10 G chemically competent cells (Lucigen). Successful transformants were miniprepped and confirmed via Sanger sequencing (UW–Madison Biotechnology DNA sequencing core facility).
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bioRxiv - Molecular Biology 2023Quote: ... coli RNase I (10 U/µL, Epicentre) digestion occurred at room temperature for 45 minutes while lysate gently rotated or mixed ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Genomics 2021Quote: ... 11 μL purified CUT&Tag-DNA was used in the reaction (11 μL DNA, 2 μL 10 μM Tn5mC-ReplO1 oligo, 2 μL 10× Ampligase buffer (Lucigen), 2 μL dNTP mix (2.5mM each ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 U/uL Rec J exonuclease (Epicentre RJ411250), and the included buffer were added and incubated at 30 C for 45 minutes.
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Microbiology 2020Quote: ... treated with 10 U Plasmid-Safe ATP-Dependent DNase (Lucigen) exonuclease at 37°C for 1 hr ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Genetics 2023Quote: ... then electroporated 10 uL into Endura competent cells (Lucigen #602422) and then expanded the cells in liquid culture for 18 hours at 30°C and purified library plasmid with the Nucleobond Xtra Midi EF kit (Machery-Nagel #740420.50) ...
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bioRxiv - Cell Biology 2024Quote: ... We treated 10 μg of RNA with RNase I (Epicentre) for 45 min at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... The eluted vRNA was treated with 10 U of RNaseR (Lucigen) in an RNaseR buffer (20 mM Tris- HCl [pH 8.0] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA extraction was done using 10 ul of QuickExtract™ (Lucigen) in a 96 well plate format and the whole fly was used to extract DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... ARTseqTM kit from Epicentre (#RPHMR12126); RNaqueous kit from Ambion (#AM1912) ...
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bioRxiv - Cancer Biology 2019Quote: ... one set of the duplicated plates was used for genomic DNA extraction using QuickExtract DNA Extraction Solution (Lucigen). 1-2μl of extracted genomic DNA in a total PCR reaction volume of 25μl were amplified in a 35 cycle PCR using a locus-specific primer set (primer set 1 ...
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bioRxiv - Bioengineering 2019Quote: Cells in 96 well plates were washed once with PBS before addition of QuickExtract™ DNA Extraction Solution (Lucigen). The volume was adjusted based on the estimated cell number ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Excess linker was degraded by adding 2 μl 10× RecJ buffer (Lucigen), 1 μl RecJ exonuclease (Lucigen) ...
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bioRxiv - Genetics 2021Quote: ... Masterpure Yeast RNA purification kit (Epicentre) was used to extract the RNA by following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: AmpliScribe SP6 Transcription Kit (Epicentre AS3106).
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Genetics 2021Quote: ... DNA extraction from 2 x 2 x 2mm fin-tissue clips was performed in a 96-well PCR plate using 100μl of QuickExtract solution (Lucigen, USA) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from 96-well plates of confluent clones using QuickExtract DNA Extraction Solution (Lucigen Corporation, Middleton, WI, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... rRNA depletion was performed (rRNA depletion Kit Ribo Zero Magnetic Kit for Gram-positive bacteria; Epicentre [Illumina]) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Ribo-ZeroTM rRNA Removal Kits (Bacteria) and Ribo-ZeroTM Magnetic Gold Kit (Yeast) (Epicentre, San Diego, CA) were used to remove rRNA from the total RNA ...
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bioRxiv - Genetics 2021Quote: ... The DNA:RNA hybrids were digested with 10 U Thermostable RNase H (Epicentre, H39500) in 50 mM Tris-HCl (pH 7.4) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl RNase H buffer and 2 μl hybridase thermostable RNase H (Lucigen) preheated to 45° were added ...
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bioRxiv - Microbiology 2021Quote: ... a combined kit for the ribosomal (rRNA) depletion Ribo-Zero™ Kit (Bacteria) (Epicentre, Illumina, Madison, WI USA) and cDNA library construction kit ...