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Citations for Lucigen :
1 - 50 of 703 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... RNA-seq library construction was carried out with the ScriptSeq™ v2 kit (Epicentre SSV21124). Final libraries were amplified with 13 PCR cycles ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA samples were used for library construction using ScriptMiner Small RNA-seq library preparation Kit (Epicentre) and sequencing was performed on an Illumina NextSeq500.
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bioRxiv - Neuroscience 2022Quote: ... The samples were processed for directional mRNA-seq library construction using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Bioengineering 2020Quote: ... for the top four guides for construction of the gRNA and were used for in vitro transcription using the AmpliScribe T7-Flash Transcription kit (Lucigen, Palo Alto, CA) and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... The DNA:RNA hybrids were digested with 10 U Thermostable RNase H (Epicentre, H39500) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA-DNA hybrids were specifically digested with Hybridase Thermostable RNase H (Epicentre) at 45 °C for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... The gRNA-msDNA hybrid was extracted with the QuickExtract RNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Total genomic DNA of hybrids was extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen). DNA concentration was measured using the Qubit dsDNA HS assay (ThermoFischer ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA libraries were circularized by two sequential CircLigase (Epicentre) reactions and amplified by 9-12 PCR cycles.
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bioRxiv - Genomics 2023Quote: ... Two 40 Kb mate-pair libraries were generated by Lucigen from 1 µg of gDNA using the CviQl and BfaI restriction enzymes ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... waltii isolates as well as the generated hybrids were extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen).
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI ...
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bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Two micrograms of starting material were treated with Baseline-ZERO™ DNase (Lucigen, USA) and ribosomal RNA was removed using the Ribo-Zero™ Magnetic Core Kit and Ribo-Zero™ rRNA Removal kit (Illumina ...
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bioRxiv - Genetics 2020Quote: ... 2 µl per tube was transformed into two tubes of 50 µl of electrocompetent cells (Lucigen DUO) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was performed in a two-step barcoded PCR protocol using the FailSafe PCR PreMix (Lucigen, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloning was carried out by adding the two 15kb fragments (80fmol each) to 40fmol pSMART BAC vector (Lucigen) and incubating them with a proprietary assembly mix (Twist ...
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bioRxiv - Microbiology 2022Quote: ... two µg of total RNA were treated with 5 U of RNase R (cat. # RNR07250, Lucigen, Middleton, WI) for 40 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA of the two parents and the F2 progeny was extracted with the QuickExtract DNA Extraction Solution (Lucigen, QE0905T) according to manufacturer’s protocol with modifications ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg of RNA was used as template for reverse transcription following the manufacturer’s instructions (EpiScript™ Reverse Transcriptase System kit, Epicentre, USA), using random hexamer primers (Fermentas ...
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bioRxiv - Microbiology 2022Quote: ... two mid-vaginal specimens were self-collected by the subject using sterile Catch-All™ Sample Collection Swabs (Epicentre Biotechnologies) and placed at −20°C ...
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bioRxiv - Molecular Biology 2022Quote: Approximately 1e+06 cells were harvested two days post nucleofection and incubated in 200 μL of QuickExtract DNA Extraction Solution (Lucigen) at 65ºC for 15 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μl per tube was transformed into two tubes of 50 μl of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... two ncRNA expression cassettes (for barcoded ncRNAs “A” and “B”) from the Marionette plasmids were cloned into pSol-TSF (Lucigen F843213-1) facing in opposite directions ...
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bioRxiv - Microbiology 2020Quote: ... were collected by swabbing the inner pinna of the ear of two dogs using Sterile Catch-All™ Sample Collection Swabs (Epicentre Biotechnologies). DNA was extracted with QIAGEN-DNeasy PowerSoil Kit ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... ARTseqTM kit from Epicentre (#RPHMR12126); RNaqueous kit from Ambion (#AM1912) ...
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bioRxiv - Molecular Biology 2020Quote: ... The pure transcripts were capped using the ScriptCap™ m7G Capping System (Epicentre). Radiolabelled transcripts were obtained by substituting the GTP from the kit with [α32P]GTP.
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bioRxiv - Genetics 2021Quote: ... Masterpure Yeast RNA purification kit (Epicentre) was used to extract the RNA by following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: AmpliScribe SP6 Transcription Kit (Epicentre AS3106).
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bioRxiv - Microbiology 2019Quote: ... rRNA depletion was performed (rRNA depletion Kit Ribo Zero Magnetic Kit for Gram-positive bacteria; Epicentre [Illumina]) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Ribo-ZeroTM rRNA Removal Kits (Bacteria) and Ribo-ZeroTM Magnetic Gold Kit (Yeast) (Epicentre, San Diego, CA) were used to remove rRNA from the total RNA ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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bioRxiv - Microbiology 2021Quote: ... a combined kit for the ribosomal (rRNA) depletion Ribo-Zero™ Kit (Bacteria) (Epicentre, Illumina, Madison, WI USA) and cDNA library construction kit ...
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bioRxiv - Genetics 2021Quote: ... ribosomal depleted using Ribo-Zero Kit (Epicentre) and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) ...
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bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre). Second ...
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bioRxiv - Plant Biology 2021Quote: ... A Ribo-Zero magnetic kit (MRZPL116, Epicentre) was used for rRNA depletion from total RNA ...
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bioRxiv - Immunology 2022Quote: ... ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre) and rRNA-free residue was obtained by ethanol precipitation ...
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bioRxiv - Microbiology 2022Quote: ATP-Dependent DNase kit (Lucigen, catalog #: E3110K).
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bioRxiv - Bioengineering 2020Quote: ... using AmpliScribe T7-Flash Transcription Kit (Lucigen) following the manufacturer’s instruction and purified by RNA Clean & Concentrator (Zymo) ...
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bioRxiv - Genomics 2021Quote: ... and the Globin-Zero Gold kit (Epicentre) to remove globin mRNA and ribosomal RNA ...
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Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA), and rRNA- free residue was cleaned up by ethanol precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Poly(A) Polymerase Tailing Kit (Lucigen, UK), Direct RNA Sequencing Kit SQK-RNA002 ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 μl of the whole cell lysate containing the digested chromatin was taken forward into an overnight blunt end ligation reaction (End-It DNA repair kit and Fast-Link DNA ligation kit, Epicentre) with double stranded DNA adapters at 16°C ...
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bioRxiv - Genomics 2020Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and the libraries have been generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Genomics 2020Quote: ... and NxSeq AmpFREE Low DNA Library Kit (Lucigen) was applied ...
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bioRxiv - Genetics 2022Quote: ... and the MasterPureTM Yeast RNA Purification Kit (Epicentre). cDNAs were synthesized with the FastQuant RT Kit (with gDNase ...