Labshake search
Citations for Lucigen :
1 - 50 of 80 citations for Tick Borne Encephalitis Virus Lysate Strain Moscow B 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... coli bacterial strain (Lucigen). Expression was induced for 1h at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... coli strain (Lucigen, EC300150). E ...
-
bioRxiv - Synthetic Biology 2020Quote: ... All strains were constructed using Escherichia coli strain C43(DE3) (Lucigen, Madison, WI). The deletion of frdABCD ...
-
bioRxiv - Molecular Biology 2019Quote: ... coli strain C41(DE3) (Lucigen) in 2×TY media supplemented with 100 μg·L−1 ampicillin ...
-
bioRxiv - Microbiology 2020Quote: ... coli strain C41(DE3)(Lucigen). Bacteria were grown at 37°C to an OD600nm of 0,9 ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli strain EPI300 (Epicentre, USA) under chloramphenicol selection for attB libraries ...
-
bioRxiv - Biophysics 2019Quote: ... E.coli strains were obtained from Lucigen; Strep-Tactin resin was acquired from Iba lifesciences and Nickel resins were purchased from ThermoFisher Scientific (HisPur™ Ni-NTA resin ...
-
bioRxiv - Biochemistry 2020Quote: ... coli C43(DE3) strain (Lucigen, WI). Protein expression was induced by 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli strain TG1 (Lucigen, Madison, WI, USA) or C3040 (NEB) ...
-
bioRxiv - Biophysics 2020Quote: ... coli cells of strain C41(DE3) (Lucigen) were transformed with the KR2 expression plasmid ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli strains Epi300 (Lucigen, Cat #: LGN-EC300110) and MC1061 (NCBI ...
-
bioRxiv - Biochemistry 2020Quote: ... Mutant proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600 ∼0.4 ...
-
bioRxiv - Biochemistry 2020Quote: ... Wild-type proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600∼0.4 ...
-
bioRxiv - Plant Biology 2022Quote: ... coli strains DH5α and C41 (DE3) (Lucigen, Middleton, WI) were grown on Luria agar medium at 37 ℃ ...
-
bioRxiv - Immunology 2023Quote: ... coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8 ...
-
bioRxiv - Pathology 2023Quote: ... coli strains DH5α and C41(DE3) (Lucigen, Middleton, WI) were grown on Luria agar medium at 37 ℃ ...
-
bioRxiv - Biochemistry 2019Quote: ... CLIC1 was expressed recombinantly in the C43 E.coli strain (Lucigen). The cells were lysed by sonication ...
-
bioRxiv - Biochemistry 2021Quote: ... CLIC1 was expressed recombinantly in the C43 E.coli strain (Lucigen). The cells were lysed by sonication ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
-
bioRxiv - Molecular Biology 2019Quote: ... purified virus RNA was treated with 5 units of tobacco acid pyrophosphatase (Epicentre) to generate 5’ monophosphorylated termini ...
-
bioRxiv - Biophysics 2021Quote: ... coli strain DH5α was isolated using the QuickExtract DNA extraction solution (Lucigen). The coding sequences of the four autoinducer-2 exporter genes were amplified using the Phusion DNA polymerase (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... the expression was conducted in the E.coli strain C41 (DE3) pLysS (Lucigen) in LBE-5052 autoinduction medium for 24 hours at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... Worms were lysed in DNA Quick Lysate (Epicentre Technologies) for 1 hour at 60°C and the lysate was then used as a template for PCR with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA was isolated from strains using a MasterPure Gram Positive DNA (Epicentre) purification kit with modifications as previously described [53] ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with 500 U of RNAse I (Epicentre) per 25 μg of RNA (quantified by Qubit fluorimetry – ThermoFisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
-
bioRxiv - Immunology 2023Quote: The Escherichia coli strains TG1 and AXE688 (21, 22, 23) were purchased from Lucigen Corporation (Middleton ...
-
bioRxiv - Molecular Biology 2024Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
-
bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Synthetic Biology 2020Quote: Golden gate assembly reactions were transformed into chemically competent Escherichia coli prepared from strain TG1 (Lucigen). Transformed cells were selected on Lysogeny Broth containing the antibiotics chloramphenicol ...
-
bioRxiv - Microbiology 2021Quote: Ribosomal RNA was removed from strain B50 transcriptomes by using the Ribo-Zero Magnetic Kit (EPICENTRE Biotechnologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4 µg RNA was digested with 4 U RNase R (Epicentre) in a total reaction volume of 10 µL for 10 minutes at 37 °C ...
-
bioRxiv - Microbiology 2019Quote: ... 10 μg of total RNA of each strain was treated with RNA 5’-Polyphosphatase (Epicentre, Madison, Wisconsin). The dephosphorylated RNAs were self-ligated by T4 RNA ligase (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: The RNAs from Mucor strains were extracted using the MasterPure™ yeast RNA purification kit (Lucigen MPY03100). Extracted RNA was then converted to cDNA by utilizing a high-capacity cDNA reverse transcription kit (Applied Biosystems™ 4374966) ...
-
bioRxiv - Biochemistry 2021Quote: ... coli DnaB and mutants (R74A, R164A, K180A, R328/329A) were independently expressed in C41 strain (Lucigen, Middelton, WI) from pET11b-derived plasmids as previously described [4] ...
-
bioRxiv - Biochemistry 2022Quote: ... the wild type or the variants of BbZIP was expressed in the strain of C41 (DE3) pLysS (Lucigen) in LBE-5052 Autoinduction medium (30 ...
-
bioRxiv - Cell Biology 2019Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
-
bioRxiv - Genomics 2020Quote: ... gDNA for the rest of the strains was extracted using the Masterpure Complete DNA and RNA purification kit (Lucigen) using the protocol for tissue samples ...
-
bioRxiv - Genomics 2022Quote: DNA was isolated from the 16 parental strains using the Masterpure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). The Pacific Biosciences protocol “Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” was used to create libraries from 30 micrograms of DNA ...
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate containing 10 µg RNA was treated with 20 U of RNase I (Lucigen) for 45 min at 25ºC ...
-
bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Single colonies of each suppressor strain were obtained and genomic DNA was prepped using MasturePure(tm) Yeast DNA Purification Kit (Lucigen). The DHR1 ...
-
bioRxiv - Microbiology 2019Quote: ... was used to create a fusion PCR product to replace fHbp in strain L91543 with the kanamycin resistance gene (kan) from EZ::Tn5
insertion kit (Epicentre) following the approach described by Horton [57] ... -
bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2022Quote: ... the Janus cassette was amplified from genomic DNA isolated from strain AZ59 (13) using the MasterPure DNA purification kit (Lucigen), with flanking regions (∼1kb ...
-
bioRxiv - Microbiology 2022Quote: ... 1 kb regions directly upstream and downstream of the hsdS open reading frame were amplified from genomic DNA of the respective strains and annealed to either side of a chloramphenicol resistance cassette (Tn5 HyperMu transposon, Epicentre) using overlap extension PCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
-
bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...