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Citations for Lucigen :
1 - 50 of 567 citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Single colonies of each suppressor strain were obtained and genomic DNA was prepped using MasturePure(tm) Yeast DNA Purification Kit (Lucigen). The DHR1 ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... The purified capped RNA was subjected to 2’-O methylation using ScriptCapTM 2’-O-Methyltransferase (Epicentre) in the presence of cold S-adenosyl methionine (SAM ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was amplified (Epicenter TargetAmp 2-Round aRNA Amplification Kit 2.0, Epicentre Biotechnologies), DNase-treated (RapidOut DNA Removal kit ...
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bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
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bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Biochemistry 2024Quote: The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3×FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates ...
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bioRxiv - Systems Biology 2022Quote: ... RNA was harvested using RNA QuickExtract (Lucigen). Turbo DNAse (Invitrogen ...
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bioRxiv - Immunology 2019Quote: 1ug of total cellular RNA was treated with RNA 5’ polyphosphatase (enzyme that converts 5’-triphosphorylated RNA into 5’-monophosphorylated RNA, Lucigen) for 30 min at 37°C or mock-treated ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of total RNA was treated with the RNA processing enzyme RNA 5′-polyphosphatase (Epicentre) to convert 5′-triphosphate RNA or 5′-diphosphorylated RNA to 5′-monophosphate RNA without dephosphorylating monophosphorylated RNA ...
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bioRxiv - Genetics 2024Quote: ... RNA was purified using RNA Clean & Concentrator - 5 kit and subsequently treated with RNA 5′ Polyphosphatase (Lucigen) for 30 minutes at 37°C ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
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bioRxiv - Cancer Biology 2023Quote: RNA-sequencing (RNA-seq) was performed using either ScriptSeq (EpiCentre) library preparation or using the KAPA RNA HyperPrep Kit (RiboErase HMR ...
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bioRxiv - Genetics 2021Quote: ... coli (Lucigen, 60242-2) by electroporation (1.8 kV ...
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bioRxiv - Neuroscience 2022Quote: ... coli (Lucigen 60242-2) using program EC1 on MicroPulser Electroporator (Bio-Rad 1652100 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB M0368X ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was extracted using MasterPure yeast RNA purification kit (Epicentre). To completely eliminate DNA contamination ...
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bioRxiv - Systems Biology 2020Quote: ... Total-RNA (250ng) was depleted of ribosomal RNA using RNaseH (Epicentre) and ribosomal RNA targeting oligonucleotides based on a protocol by Adiconis et al. ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was prepared using the MasterPure RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of eluent was then electroporated into each of 2 tubes of 50 uL Endura electrocompetent cells (Lucigen, Cat#60242-2) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA extraction was done using the ‘MasterPure Yeast RNA Purification Kit’ (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... RNA extraction was performed using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized from the extracted RNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermoscientific) ...
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bioRxiv - Cell Biology 2019Quote: RNA extraction was performed using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermoscientific) ...
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bioRxiv - Genetics 2021Quote: ... RNA samples for small RNA-seq were treated with 5’polyphosphatase (Lucigen) and prepared using the SMARTer Apollo system with modifications for small RNA library preparation ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA extractions were performed using MasterPure™ RNA Purification Kit (Epicentre®) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg RNA (harvested using the MasterPure Yeast RNA Purification Kit (Epicentre)) was DNAse treated with the Turbo DNA-free Kit (Invitrogen) ...
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bioRxiv - Systems Biology 2019Quote: ... total RNA was extracted with a MasterPure Yeast RNA Purification kit (Epicentre) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: RNA was extracted using the MasterPure™ RNA Purification Kit (Epicentre, #MC85200). Cells were analyzed for messenger RNA (mRNA ...
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bioRxiv - Genomics 2019Quote: RNA from 100,000 K562 cells was extracted with RNA QuickExtract (Lucigen QER090150). RNA was treated with DNaseI from the same kit ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen). First strand cDNA synthesis was performed using the EasyScript Plus cDNA Synthesis Kit (Lamda Biotech ...
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bioRxiv - Microbiology 2023Quote: Total RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized using the PrimeScript RT Master Mix (Takara).
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bioRxiv - Cell Biology 2024Quote: RNA extraction was performed using the MasterPure Yeast RNA purification kit (Lucigen) from samples treated as indicated in the legend of Figure S1 ...
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bioRxiv - Cancer Biology 2019Quote: ... coli (Lucigen, cat. 60242-2) using Bio-Rad MicroPulser Electroporator (cat ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
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bioRxiv - Immunology 2020Quote: ... coli (Lucigen, Cat# 60502-2) over fifty shocks for each library ...
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bioRxiv - Microbiology 2024Quote: ... 2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA was purified using the MasterPure™ Yeast RNA Purification Kit from Epicentre. cDNA was synthesized with random hexamer primers using SuperScript® IV reverse transcriptase ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre), and enriched mRNA was applied to library preparation according to manufacturer’s protocol (NEBNext Ultra) ...