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Citations for Lucigen :
1 - 50 of 50 citations for Scyliorhinin II amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... circularized using CircLigase II (EpiCentre), annealed to the iCLIP Cut Oligo ...
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bioRxiv - Developmental Biology 2021Quote: ... then circularised using Circligase II (Epicentre) followed by AMPure XP beads purification ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... circularized with CircLigase II (CL9025K, Epicentre), and PCR-amplified using Phusion polymerase (M0530S ...
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bioRxiv - Microbiology 2020Quote: ... Next 5 μL of circularization reaction buffer was added (3.3 μL water, 1.5 μL 10x Circligase-II buffer, and 0.5 μL of Circligase-II (Epicentre)) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was resuspended in 7 μl of water and then 8 μl of CircLigase II mix (5 μl water, 1.5 μl 10x CircLigase Buffer II, 0.75 μl 50 mM MnCl2, 0.75 μl Circligase II [Lucigen]) were added ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... and then circularized using CircLigase II (Epicentre). Two rounds of PCR were then performed to first amplify the library using adaptor-specific primers and to add sequences compatible with Illumina sequencing instruments ...
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bioRxiv - Neuroscience 2020Quote: ... and circularized with CircLigase II (Epicentre CL9025K). RPF libraries were PCR amplified with Phusion High-Fidelity DNA Polymerase (NEB M0530A ...
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bioRxiv - Genetics 2020Quote: ... and subsequently circularised with CircLigase II (Epicentre). PCR amplification was obtained after 18-21 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... Fragments were circularized by CircLigase II (Epicentre), recovered by ethanol precipitation ...
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bioRxiv - Genomics 2019Quote: ... After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme, CL9025K, Epicentre) was added to the sample and incubated for two hours at 60° C ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... circularized by CircLigase™ II ssDNA ligase (Lucigen). Subsequently ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was circularized with CircLigase II (CL9021, Lucigen). Libraries were PCR amplified using Phusion DNA Polymerase (M0530 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recovered cDNA was circularized with Circligase™ II (Epicentre) at 60 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were circularized using circligase II (#CL9025K, Epicentre). The major rRNA contaminants were removed using subtractive hybridization with custom biotinylated oligos (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was then circularized using Circligase II (Lucigen, #CL4111K) and then linearized using BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... and the RT product was circularized by CircLigase II (Epicentre). A PCR library was amplified and sequenced using Illumina Nextseq 500 at the Rockefeller University Genomics Center.
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bioRxiv - Molecular Biology 2022Quote: ... The purified cDNAs were then ligated using CircLigase II (Lucigen) and PCR-amplified to generate the sequencing library.
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bioRxiv - Microbiology 2022Quote: ... Linear single stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Neuroscience 2022Quote: ... cDNA was size selected and circularized with Circligase II (Epicentre). Circularized cDNA was cut with BamHI and amplified using Accuprime Supermix I (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Linear single-stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Purified cDNA was circularized using CircLigase II enzyme (Epicentre, CL9025K), then library PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al ...
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bioRxiv - Synthetic Biology 2020Quote: ... The synthetic template DNA (IDT) was circularized using CircLigase II (Epicentre), treated with Exonuclease I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Immunology 2023Quote: ... Circular ligation was performed using CircLigase II ssDNA Ligase (Epicentre CL4111K) for 1,5 hours at 60°C ...
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bioRxiv - Genomics 2023Quote: ... and gel- purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described36 and libraries constructed using a different reverse indexing primer for each sample.
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bioRxiv - Cell Biology 2024Quote: ... Eluted cDNA was circularized with CircLigase II ssDNA ligase (Lucigen, CL9021K) for 1 hr at 60°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was circularized by CircLigase™ II ssDNA Ligase (Epicentre, CL9025K). Next ...
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bioRxiv - Microbiology 2022Quote: ... Gel-purified cDNAs were then circularized with CircLigase II (Lucigen, Cat: CL4115K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4μL 50mM MnCl2 and 0.3μL of 100U/μl CicrLigase II ssDNA ligase (Epicentre CL9021K) and incubated in a PCR machine at 60°C for 60 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 10pmol of template oligo was cyclized and ligated using CircLigase II ssDNA Ligase (Lucigen) supplemented with 2.5mM MnCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of circularization reaction mix containing 5 units/µL CircLigase II (Lucigen, CL9021K), 1× CircLigase II buffer (Lucigen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were size-purified on TBE-Urea gels before being circularized by CircLigase II (Epicentre). Circularised cDNAs were then annealed to an oligonucleotide complementary to the BamHI site and then BamHI digested ...
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bioRxiv - Molecular Biology 2021Quote: Eluted cDNA was moved to a new PCR tube and mixed with CircLigase II (Epicentre) reaction mix containing ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified linearized DNA was ligated to the JEBPN-DNA linker (Table S6) using Circligase II (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were hydrolyzed by addition of 250mM NaOH and the cDNAs were circularized using CircLigase II (Lucigen). The circularized cDNAs were sent to Genomics Core at Scripps Florida for deep sequencing using either single-end or pair-end Illumina NextSeq 500 platform.
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bioRxiv - Systems Biology 2019Quote: ... Ligation of the 5’ adapter (P5_phospho_adapter, oligo 39) to the cDNA was performed using CircLigase II (Lucigen, CL9021K) for 6 hours at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was later hydrolyzed by 250 mM NaOH and the cDNA was circularized using CircLigase II (Lucigen). The circularized cDNA was shipped to the Scripps La Jolla Genomics Core for final library preparation with Illumina sequencing adaptors and sequenced using single-end sequencing on the Illumina NextSeq 500 platform.
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bioRxiv - Microbiology 2024Quote: ... and the DNA adaptor was added for cDNA adaptor ligation using CircLigase™ II ssDNA Ligase (CL9021K, Lucigen) as described by the product instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Circular ssDNA substrate was prepared by circularisation of 5’-32P labelled TK-49 using CircLigase II (Lucigen cat#CL9021K), according to manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...