Labshake search
Citations for Lucigen :
1 - 50 of 462 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... The human gDNA was isolated from U2OS cells using the QuickExtract™ DNA Extraction Solution (EPICENTRE). From the gDNA ...
-
bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
-
bioRxiv - Molecular Biology 2019Quote: ... rRNA was depleted using the Ribo-Zero kit Human/Mouse/Rat (Epicentre), and libraries were prepared using random priming ...
-
bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
-
bioRxiv - Genomics 2019Quote: ... Gram-negative and Human/mouse/rat Ribo-Zero rRNA Removal Kit (Epicentre Technologies). The resulting RNA was purified using Zymo RNA clean & Contentrator-5 column kit (ZYMO Research) ...
-
bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
-
bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
-
bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5µg of total RNA was subjected to rRNA depletion using the RiboZero Human/Mouse/Rat kit (Epicentre Biotechnologies). cDNA was generated from the depleted RNA using random hexamers or custom primers and Superscript III (Life Technologies ...
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was subjected to ribosomal RNA depletion using the Ribo-Zero Magnetic Gold Kit (Human/Mouse/Rat; Epicentre), according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... coli cells (Endura electrocompetent cells, Lucigen) following the manufacturer’s instructions transforming 90 ng of library DNA into 25 μL of electrocompetent cells for each electroporation reaction for a total of 6 reactions and the cells were pooled and plated on 6 x LB agar plates (245-mm square bioassay dish ...
-
bioRxiv - Neuroscience 2019Quote: ... The deletion of the Tet2 gene was determined by analysis of DNA extracted from isolated primary microglia using a QuickExtract™ (Epicentre) and amplified with MyTaq™ Red DNA Polymerase (Bioline).
-
bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... about 15 μg of total RNA of human frontal cortex were treated with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C and purified by phenolchloroform extraction ...
-
bioRxiv - Molecular Biology 2022Quote: ... were sent to BGI (Hongkong) for ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) followed by library construction using non-stranded (Replicate 1 ...
-
bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) and purified as described by Urbanek et al ...
-
bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
-
bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed by electroporation ...
-
bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed with the resulting plasmid by electroporation ...
-
bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
-
bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
-
bioRxiv - Biochemistry 2021Quote: ... coli cells (Lucigen). Cells cultured in SOC media and recovered for 45 minutes at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... TG1 cells (Lucigen) were transformed with this library by electroporation.
-
bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen) and then cultivated at 37°C in LB medium supplemented with kanamycin and streptomycin ...
-
bioRxiv - Systems Biology 2020Quote: ... coli cells (Lucigen) using a GenePulserXCell (Biorad ...
-
bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) were transformed with expression plasmid and a single colony was used to start an overnight culture in LB media ...
-
bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
-
bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen). To allow for fluorescent labeling of Wsp1p-PRD ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli cells (Lucigen), which were plated on LB agar supplemented with 100 µg mL−1 ampicillin ...
-
bioRxiv - Biochemistry 2020Quote: ... coli cells (Lucigen) harboring the pTA-MOB conjugation helper plasmid (Strand et al. ...
-
bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... coli cells (Lucigen). Integration ...
-
bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen), and recovered for one hour at 37°C in Recovery Media (Lucigen) ...
-
bioRxiv - Microbiology 2019Quote: ... cloni electrocompetent cells (Lucigen) according to manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: ... cloni® cells (Lucigen) and plated on 37 large agar plates with Kanamycin antibiotics in order to conserve library complexity ...
-
bioRxiv - Genetics 2021Quote: Endura electrocompetent cells (Lucigen) were electroporated with the genome-scale CRISPR KO (Brunello ...
-
bioRxiv - Bioengineering 2021Quote: ... coli electrocompetent cells (Lucigen) then plated on a 245 × 245 mm LB and kan agar plate ...
-
bioRxiv - Biochemistry 2022Quote: ... coli competent cells (Lucigen). A single colony of transformed cells was grown in 3 mL lysogeny broth (LB ...
-
bioRxiv - Molecular Biology 2020Quote: C41(DE3) cells (Lucigen) were transformed with the expression plasmid using standard techniques ...
-
bioRxiv - Biochemistry 2022Quote: ... cloni 10G cells (Lucigen) by electroporation (1.8 kV) ...
-
bioRxiv - Bioengineering 2022Quote: ... coli SS320 cells (Lucigen) containing the circular phagemid comprising the target scaffold sequence and a M13cp helper plasmid ...