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Citations for Lucigen :
1 - 50 of 102 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
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bioRxiv - Microbiology 2024Quote: ... the amplified DNA was cloned into the pETite N-His vector (Expresso™ T7 cloning and expression system, Lucigen, # 49001-1), resulting in constructs with an N-terminal 6x His tag ...
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bioRxiv - Genomics 2020Quote: T7 RNA polymerase (Lucigen NxGen)
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bioRxiv - Molecular Biology 2021Quote: ... 50U T7 RNA Polymerase (Lucigen), 1 μL RNase Inhibitor and 50~100 ng DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Genetics 2022Quote: ... and NxGen T7 RNA polymerase (Lucigen) at 37 °C for 18 hours ...
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... using AmpliScribe T7-Flash Transcription Kit (Lucigen) following the manufacturer’s instruction and purified by RNA Clean & Concentrator (Zymo) ...
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bioRxiv - Neuroscience 2019Quote: ... 20pg of in vitro transcribed RNA synthesized from the T7 control DNA Template (AmpliScribe™ T7 Transcription Kit, Epicentre) was added to the samples ...
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bioRxiv - Genetics 2021Quote: ... columns and approximately 120–150 ng of DNA was used as template for a T7 in vitro transcription (IVT) reaction (AmpliScribe-T7-Flash transcription kit from Epicentre). In vitro transcribed gRNAs were DNAse-treated using TURBO-DNAse for 20 min at 37 °C and precipitated with sodium acetate/ethanol and resuspended in RNAse and DNAse free water ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 U/µl NextGen T7 RNA polymerase (Lucigen), 1 mM of each rNTP (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507, Lucigen) was used according to the manufacturer protocol with 4 μL PCR products per 20 μL reaction ...
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were then in vitro transcribed with T7 polymerase (Lucigen). Capture SELEX was then carried out as follows ...
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bioRxiv - Microbiology 2022Quote: ... sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre), DNase treated ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were then synthesized using the AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified by ethanol precipitation or with RNeasy MinElute Cleanup Kit (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... The AmpliScribe™ T7 High Yield Transcription Kit was purchased from Epicentre and the HiScribe T7 In Vitro Transcription Kit was obtained from New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... was synthesized using in vitro transcription (AmpliScribe T7-Flash Transcription Kit, Lucigen) and was then installed with 5’cap (Vaccinia Capping System ...
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bioRxiv - Evolutionary Biology 2023Quote: ... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Bioengineering 2021Quote: ... PCR fragments were then in vitro transcribed using the AmpliScribe T7-Flash kit (Lucigen) with overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Developmental Biology 2024Quote: ... The dsRNA was synthesized using the AmpliScribe T7 High Yield Transcription Kit (Lucigen, USA). Primer sequences for the PCR amplification are provided in Supplementary Table S3.
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bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Genomics 2019Quote: ... The resulting template was transcribed using the Lucigen AmpliScribe T7-flash Transcription Kit from Epicentre/Illumina (cat ...
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bioRxiv - Microbiology 2019Quote: ... 200 ng of template DNA were transcribed using the AmpliScirbe T7 Flash Transcription Kit (Epicentre) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and in vitro transcription performed using the T7-Flash BiotinRNA Transcription Kit (Epicentre, biotin labelling) or TranscriptAid T7 High Yield Transcription Kit (ThermoScientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... shRNAs were synthesized with AmpliScribe™ T7-Flash™ Transcription Kit (Epicentre, Charlotte, NC, USA) for 15 h followed by 15 min treatment with 1 μl of DNAse I ...
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bioRxiv - Microbiology 2022Quote: ... In vitro transcription was performed using AmpliScribe T7-Flash Transcription kit (Lucigen Corp., Middleton, USA), in the presence of 32P-labeled nucleotides ...
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bioRxiv - Molecular Biology 2022Quote: ... 2459 bp) were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). Curlcake 2 was polyadenylated using E ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Molecular Biology 2020Quote: ... The ‘curlcake’ constructs were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507) with either unmodified rNTPs (UNM) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... this mixture was mixed with the components of AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen, USA) and incubated for eight hours at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of shRNAs was performed using the AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen). The transcription reaction was allowed to continue at 37°C for 5-7h ...
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bioRxiv - Genomics 2023Quote: ... Linearized plasmids were used for in vitro transcription with the AmpliScribe T7-Flash Transcription Kit (Lucigen, ASF3507) using 5-Methyluridine-5’-Triphosphate (5-mUTP ...
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bioRxiv - Plant Biology 2019Quote: Each ARF DBD was cloned in the pETite N-His SUMO Kan expression vector (Lucigen, WI) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al ...
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bioRxiv - Bioengineering 2021Quote: ... The dsDNA library was then converted to modified-base RNA using the Durascribe T7 Transcription Kit (Lucigen, MA170E). This kit produces RNA that is resistant to RNase A degradation through the replacement of canonical CTP and UTP with 2’-fluorine-dCTP (2’-F-dCTP ...
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bioRxiv - Plant Biology 2021Quote: ... complementary RNA (cRNA) was prepared with the AmpliCap-Max™ T7 High Yield Message Maker Kit (Epicentre Biotechnologies). Oocyte preparation and cRNA injection were performed as previously described [94] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al. ...
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bioRxiv - Molecular Biology 2019Quote: In vitro transcribed (IVT) sequences were produced using the Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507), using 1 ug of purified digestion product as starting material ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... sgRNA was synthesized in vitro as per the suggested protocol using Ampliscribe T7 Flash Transcription kit (Lucigen, USA). 6xHis-Cas9 protein was synthesized as described previously25 ...
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bioRxiv - Biophysics 2023Quote: Viral UTR RNA and its variants were made by in vitro transcription (IVT) (AmpliScibeTM T7-FlashTM, ASF3257, Lucigen) using a template containing a T7 promoter made from PCR off a plasmid encoding full-length gRNA sequence ...