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Citations for Lucigen :
1 - 50 of 131 citations for Human CMTR2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Evolutionary Biology 2020Quote: ... shRNAs were synthesized with AmpliScribe™ T7-Flash™ Transcription Kit (Epicentre, Charlotte, NC, USA) for 15 h followed by 15 min treatment with 1 μl of DNAse I ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of shRNAs was performed using the AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen). The transcription reaction was allowed to continue at 37°C for 5-7h ...
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bioRxiv - Neuroscience 2020Quote: ... Ligated plasmid was then treated with Plasmid-Safe exonuclease (Lucigen), to prevent unwanted recombination products ...
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bioRxiv - Biophysics 2023Quote: The plasmid pETite (Lucigen) containing the recombinant VHL (M54 – D213 ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid pETite (Lucigen) containing the recombinant VHL (M54 – D213 ...
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bioRxiv - Evolutionary Biology 2023Quote: The shRNA and mimiRs were transcribed according to the manufacturer’s instructions using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen, USA) with a few changes ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μl Plasmid-Safe Enzyme (Lucigen), and 6 μl 10mM ATP (New England Biolabs) ...
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bioRxiv - Genomics 2024Quote: ... Plasmid-Safe ATP-dependent DNase (Lucigen) and Exonuclease III (NEB) ...
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bioRxiv - Genetics 2023Quote: ... and Plasmid-Safe ATP-dependent DNase (Lucigen) to enzymatically degrade remaining linear DNA molecules ...
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bioRxiv - Molecular Biology 2023Quote: ... either into pCC1FOS™ plasmid (Epicentre, USA) for attB libraries ...
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bioRxiv - Cancer Biology 2020Quote: ... HBV plasmid (positive control) and HBV DNA from inoculum was digested with plasmid-safe DNase (Epicentre, E3101K, Madison, WI). According to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
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bioRxiv - Cell Biology 2021Quote: ... pSMART-HC-Amp plasmid was purchased from Lucigen. pET28-12XHis-SUMO plasmid was from Xudong Wu.
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bioRxiv - Immunology 2023Quote: The pJAZZ-OK-based linear bacterial plasmids (Lucigen) with coding regions of every HAs were used as templates for mRNAs production ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid-Safe ATP-Dependent DNase (Lucigen, WI, USA) was added to the purified DNA to remove double-stranded and circular DNA ...
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bioRxiv - Genetics 2021Quote: ... a restriction digest is performed to exclusively linearize the parental plasmid followed by an overnight digestion with Plasmid-Safe™ ATP-Dependent DNase (Lucigen). Prior to MIJ ...
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bioRxiv - Microbiology 2019Quote: ... The Plasmid-Safe ATP-dependent exonuclease (Epicentre, E3101K, USA) kit was used to ensure that mainly circular DNA elements were left in the sample ...
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bioRxiv - Genomics 2020Quote: ... TOPO plasmids were electroporated into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid-Safe ATP-Dependent DNase was purchased from Epicentre Technologies Corp ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmid-safe ATP-dependent DNase was obtained from Lucigen, WI ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid-Safe™ ATP-Dependent DNase (#E3101K) from Epicentre; PolyJet™ reagent from SignaGen Laboratories ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid pHCKan-yibDp-GFP was constructed as described previously.10 Plasmid pHCKan-yibDp-tsGFP was assembled from a gBlock™ and store-bought linearized pHCKan plasmid (Lucigen, Middleton, WI) using NEBuilder® HiFi DNA Assembly Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid-Safe ATP-Dependent DNase was from Lucigen (Middleton, WI). Surveyor Mutation Detection Kit S100 was from Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2020Quote: ... treated with 10 U Plasmid-Safe ATP-Dependent DNase (Lucigen) exonuclease at 37°C for 1 hr ...
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bioRxiv - Genomics 2019Quote: ... and linear DNA was digested by plasmid safe (Epicentre, Illumina) at 37°C for 1.5 h ...
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bioRxiv - Cell Biology 2020Quote: ... four units of exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre) was added to the reaction at 37°C (reaction volume 40 µL ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Molecular Biology 2019Quote: ... rRNA was depleted using the Ribo-Zero kit Human/Mouse/Rat (Epicentre), and libraries were prepared using random priming ...
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bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
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bioRxiv - Genomics 2020Quote: ... 400 ng of pooled plasmid DNA was electroporated into Endura (Lucigen) E ...
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bioRxiv - Microbiology 2019Quote: ... and 20U/mL exonuclease V (Epicentre Plasmid-Safe ATP-dependent DNase) with overnight incubation at 37°C to promote maturation of capsids ...
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bioRxiv - Cancer Biology 2020Quote: ... The linear DNA was then removed using Plasmid-Safe DNase (Lucigen). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligated pUSEPR plasmid DNA was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37⁰C ...
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bioRxiv - Genomics 2023Quote: ... Each sample was subjected to Plasmid-Safe ATP-dependent DNase (Epicentre) digestion and performed rolling circle amplification by Phi29 DNA polymerase with Exo-resistant random primer followed by Nanopore sequencing according to the manufacturer’s protocol (Figure 1A ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was maintained in 10G elite Escherichia coli bacteria (Lucigen).
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bioRxiv - Genomics 2019Quote: ... Gram-negative and Human/mouse/rat Ribo-Zero rRNA Removal Kit (Epicentre Technologies). The resulting RNA was purified using Zymo RNA clean & Contentrator-5 column kit (ZYMO Research) ...
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bioRxiv - Cancer Biology 2019Quote: ... and treated with Plasmid-Safe(tm) ATP-Dependent DNase (Lucigen, cat. E3101K). The reaction was again concentrated by magnetic bead purification and transformed into Lucigen Endura(tm ...
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bioRxiv - Genomics 2020Quote: ... DNA was treated with 10U of Plasmid-safe Dnase (Epicentre® Illumina) for 45 minutes at 37°C following thelatest update of the international working group on cccDNAstandardization (Allweisset al. ...
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bioRxiv - Immunology 2019Quote: The recombinant plasmids were transformed into ClearColi-BL21 (DE3) electrocompetent cells (Lucigen) by heat-shock transformation ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... BAC plasmid was delivered into BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen) by electroporation and bacteria was propagated according to the manufacturer’s guide ...
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bioRxiv - Cancer Biology 2023Quote: ... the genomic DNA was treated with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... the circularization mixture was combined with 6 μl 10X Plasmid-Safe Buffer (Lucigen), 2 μl Plasmid-Safe Enzyme (Lucigen) ...
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bioRxiv - Microbiology 2021Quote: ... fragment 4 was subsequently subcloned into a low-copy plasmid pSMART LCAmp (Lucigen) to increase stability ...
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bioRxiv - Microbiology 2021Quote: ... Linear chromosomal DNA was reduced by Plasmid-Safe ATP-Dependent DNase (Epicentre, USA) treatment for 24 hours at 37°C according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...