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Citations for Lucigen :
1 - 50 of 90 citations for FABP2 I FABP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNase I (Epicentre) digestion was performed according to the manufacture’s instruction to minimize contamination with plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligase I (Lucigen) at 68°C for 2 h ...
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bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Cancer Biology 2023Quote: ... 5U/uL CircLigase I (Lucigen)) at 60C for 3 hours with heat inactivation at 80C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.25 units RNase I (Epicentre) was added to 5ul of sample in a total volume of 50 ul in IP buffer supplemented with 0.1% Igepal ...
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bioRxiv - Microbiology 2023Quote: ... treated with DNAase I (Lucigen), and reverse transcribed using AMV RT (Promega ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Molecular Biology 2020Quote: ... and 8U of DNase I (Epicentre). These diluted nuclear extracts were pre-cleared using 25µL of previously blocked Streptavidin Sepharose beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
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bioRxiv - Molecular Biology 2023Quote: ... coli RNase I (10 U/µL, Epicentre) digestion occurred at room temperature for 45 minutes while lysate gently rotated or mixed ...
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bioRxiv - Microbiology 2023Quote: ... Samples were treated with DNase I (Lucigen). Reverse transcription was performed using AMV RT (Promega ...
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bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 U/ml RNase I (Lucigen, N6901K) for 30 minutes at room temperature with gentle mixing ...
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bioRxiv - Genomics 2023Quote: ... Circularization was performed with CircLigase I (Epicentre #CL4111K) instead of CircLigase II ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Biochemistry 2020Quote: ... Illumina adapters were ligated using Circ Ligase I (Lucigen) and linker pA-Adapt-Bp (Table S5) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Plant Biology 2019Quote: Each ARF DBD was cloned in the pETite N-His SUMO Kan expression vector (Lucigen, WI) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with 500 U of RNAse I (Epicentre) per 25 μg of RNA (quantified by Qubit fluorimetry – ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs were circularized using 100U CircLigase I ssDNA ligase (Epicentre), and cDNA concentration was quantified by QPCR of cDNA compared to a standard curve of a reference sample ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Cell Biology 2024Quote: ... We treated 10 μg of RNA with RNase I (Epicentre) for 45 min at 25°C ...
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bioRxiv - Plant Biology 2024Quote: ... The CDS encoding FLVA (residues 55-723) was cloned into pETite® C-His Kan Vector (Lucigen) downstream of a sequence for a Strep-tag II (WSHPQFEK ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro transcribed RNA was then incubated with DNAse I (Lucigen), followed by purification using RNeasy Mini Kit (Qiagen-74104) ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with DNase I (cat# D9905K; Lucigen, Middleton, WI, USA). The concentration and purity of the isolated RNA were determined using a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA products were treated with Baseline-ZERO DNase I (Epicentre) and then purified again using the RNeasy Mini kit.
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bioRxiv - Genomics 2020Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, Cat: CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Genomics 2021Quote: ... Following DNAse I digestion using the Baseline-ZERO Kit (Epicentre, Madison, Wisconsin), the RNA concentration was quantified using the Qubit RNA HS Assay Kit on a Qubit Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, cat. CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg/mL cycloheximide) and incubated with 15 U RNase I (Lucigen) for 45 minutes while rotating at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by reaction clean up with exonuclease I (Lucigen, 37C for 30 minutes), RNase I/Hybridase (Lucigen ...
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bioRxiv - Biochemistry 2023Quote: ... and the transcription was terminated with the addition of DNase I (Lucigen, ASF3507). RNA targets were purified with Monarch® RNA Cleanup Kit (T2040L ...
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bioRxiv - Microbiology 2024Quote: ... the amplified DNA was cloned into the pETite N-His vector (Expresso™ T7 cloning and expression system, Lucigen, # 49001-1), resulting in constructs with an N-terminal 6x His tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate containing 10 µg RNA was treated with 20 U of RNase I (Lucigen) for 45 min at 25ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K) for 45 minutes at RT with light shaking ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... TEV eluate containing 20 µg of total RNA was treated with 10 units of RNase I (Epicentre) at 24 ºC for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...