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Citations for Lucigen :
1 - 50 of 377 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: The genomic DNA of individual control or Dll-KO embryos (sorted manually based on injection marker or on reporter expression) were extracted with 20 μl of Quick Extract ™ DNA Extraction Solution (Lucigen, Cat# QE09050) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... media was aspirated and 50-100 μL QuickExtract™ DNA Extraction Solution (Lucigen) were added to each well ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) to the cells ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre, Cat # QE09050) to the cells ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA extraction using QuickExtract DNA Extraction Solution (Lucigen) was performed according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of library was added to 25 µL of Endura electrocompetent cells (Lucigen). Cells were electroporated using the MicroPulser (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... Transposomes were prepared using 100 ng transposon DNA and EZ-Tn5 transposase (Lucigen, USA) according to the suppliers specifications ...
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bioRxiv - Microbiology 2024Quote: ... with 100 U/ml of OmniCleave nuclease (Epicentre) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Molecular Biology 2023Quote: Genomic DNA extraction was performed using QuickExtract™ DNA Extraction Solution (Lucigen), and the genomic locus of interest was amplified using AmpliTaq Gold® 360 Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Bioengineering 2019Quote: ... DNA extraction was performed using QuickExtract DNA extraction solution (Epicentre, Illumina, Madison, WI) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... extraction of genomic DNA was performed with QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Knockout clones were confirmed by genomic DNA extraction using QuickExtract DNA extraction solution (Epicentre), PCR amplification of the target locus (forward primer ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was spun down at 5,000 x g for 5 minutes and the pellet was resuspended in 100 µl of QuickExtract™ DNA Extraction Solution (Lucigen) and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Physiology 2021Quote: ... mRNA was purified from 100 ng of total RNA by using a Ribo-Zero rRNA removal kit (Epicentre) to deplete ribosomal RNA and convert into double-stranded complementary DNA by using an NEBNext mRNA Second Strand Synthesis Module (E6111L) ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction (Lucigen, Middleton, WI) was performed according to manufacturer’s instructions and gDNA content was quantified using a nanodrop (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: Quick extract DNA extraction solution (Lucigen) was tested in accordance with the manufacturer’s suggested buffer to sample ratio ...
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bioRxiv - Microbiology 2021Quote: ... and DNA extraction with QuickExtract (EpiCentre) was performed ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg/mL cycloheximide) and incubated with 15 U RNase I (Lucigen) for 45 minutes while rotating at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... one set of the duplicated plates was used for genomic DNA extraction using QuickExtract DNA Extraction Solution (Lucigen). 1-2μl of extracted genomic DNA in a total PCR reaction volume of 25μl were amplified in a 35 cycle PCR using a locus-specific primer set (primer set 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Microbiology 2019Quote: ... 50 ml of culture was used for DNA extraction using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA).
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bioRxiv - Cancer Biology 2019Quote: ... containing 30μl QuickExtract DNA Extraction Solution (Lucigen). A full plate containing the reaction mixture (single colony + extraction solution ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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bioRxiv - Developmental Biology 2020Quote: ... Cell debris was then pelleted and used for gDNA extraction with 10-20 µl QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Cell Biology 2022Quote: ... listed in Table S2 or previously described [89] and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre). All PCR amplified products were confirmed by sequencing (Eurofins MWG Operon ...
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bioRxiv - Genomics 2022Quote: ... and diluted at 1:100 ratio into 250 ml LB supplemented with kanamycin (50 μg/mL) and 1x copy number induction solution (Lucigen, CCIS125). Payload DNA was isolated from E ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was extracted (DNA extraction solution, Epicentre Biotechnologies) and edited regions were specifically amplified by PCR (primers are listed in Supplementary Table 1) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Genomics 2020Quote: ... Dual extraction of nucleic acid (RNA and DNA) from each pupa was carried out using the MasterPure dual extraction kit (Epicentre, MC85200). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Clones with the best response to TMP were expanded and lysed for gDNA extraction and purification using the QuickExtract DNA extraction solution (Lucigen, Middleton, WI). Genomic ecDHFR was amplified (forward ...
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bioRxiv - Cell Biology 2019Quote: ... it was extracted (QuickExtract DNA extraction solution, Epicentre Biotechnologies), the region of interest was amplified by PCR (primers described in Supplemental Table 4) ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... DNA was extracted (QuickExtract DNA extraction solution; Epicentre Biotechnologies), the region of interest was amplified by PCR (primers summarized in Table S3) ...
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bioRxiv - Molecular Biology 2023Quote: QuickExtract DNA Extraction Solution (Lucigen, cat. No. LGN-QE09050)
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNAs were prepared using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen), depending on confluency 25 to 100 μl of solution was added to the wells containing the cells for 15 minutes at 37C ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted with QuickExtract DNA extraction solution (EpiCentre) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...