Labshake search
Citations for Lucigen :
1 - 17 of 17 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: A Salmonella transposon mutant library containing circa 100,000 mutants was generated using EZ-Tn5 transposase (Epicentre) and the aphA1 kanamycin resistance gene as described previously (Langridge et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... Mutant proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600 ∼0.4 ...
-
bioRxiv - Microbiology 2019Quote: ... subvibrioides ΔdivK mutant using the EZ-Tn5
TNP transposome (Epicentre). B ... -
bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from candidate mutants with the MasterPure DNA purification kit (Epicentre) and PCR followed by gel electrophoresis confirmed correct integration of the NAT dominant resistance marker ...
-
bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Biochemistry 2024Quote: ... The CRISPR/Cas9-edited mutants were validated by DNA extraction using QuickExtract solution (Lucigen, QER090150) followed by sanger sequencing prior cell line expansion28,38 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
-
bioRxiv - Microbiology 2024Quote: A transposon mutant library in pJBG007 was made using EZ-Tn5
Insertion Kit (Lucigen). The following components were used for a 10µl reaction (EZ-Tn5 10X Reaction Buffer (1µl ... -
bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Genetics 2021Quote: ... and WNT11 Del and LoxP mice mutants was performed by using EconoTaq PLUS GREEN Master mix (Lucigen). The following primers were used for genotyping:
-
bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Biochemistry 2021Quote: ... coli DnaB and mutants (R74A, R164A, K180A, R328/329A) were independently expressed in C41 strain (Lucigen, Middelton, WI) from pET11b-derived plasmids as previously described [4] ...
-
bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
-
bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ...