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Citations for Lucigen :
1 - 50 of 759 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Klenow DNA Polymerase (KP810250, Epicentre, Madison, Wisconsin), and T4 Polynucleotide Kinase (EK0031 ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping was achieved by polymerase chain reaction with EconoTaq® DNA polymerase (Lucigen). Primers for genotyping of CEP164 mice ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Developmental Biology 2020Quote: ... complementary tandem repeats were generated through rolling-circle amplification (RCA) with NxGen® phi29 DNA Polymerase (Lucigen, 30221). The single-stranded amplicons were detected with a Cy5-labeled oligonucleotide (Cy5-DP5 ...
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bioRxiv - Genomics 2023Quote: ... The MDA reactions were prepared using RepliPHI Phi29 DNA polymerase and Reagent set (Epicentre, Illumina, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... C-circle and G-circles assays were performed as before except that NxGen phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Molecular Biology 2021Quote: ... C-circle and G-circles assays were performed as before except that NxGen® phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Genomics 2020Quote: T7 RNA polymerase (Lucigen NxGen)
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bioRxiv - Microbiology 2019Quote: ... coli RNA polymerase holoenzyme (Epicentre) in the transcription buffer recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... 50U T7 RNA Polymerase (Lucigen), 1 μL RNase Inhibitor and 50~100 ng DNA template ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). For embryonic genotyping during primary culture preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... coli RNA polymerase σ70 holoenzyme (Epicentre) or S ...
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bioRxiv - Genetics 2022Quote: ... and NxGen T7 RNA polymerase (Lucigen) at 37 °C for 18 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Poly(A) Polymerase Tailing Kit (Lucigen, UK), Direct RNA Sequencing Kit SQK-RNA002 ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 U/µl NextGen T7 RNA polymerase (Lucigen), 1 mM of each rNTP (NEB) ...
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bioRxiv - Genomics 2019Quote: ... Library #3 was amplified using pyrophage polymerase (Lucigen, Middleton, WI).
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bioRxiv - Developmental Biology 2023Quote: ... and tailed with a poly(A) polymerase tailing kit (Epicentre) and purified with RNA clean & concentrator-25 kit (Zymo Research).
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bioRxiv - Biophysics 2020Quote: ... Rolling circle amplification was performed by using phi29 polymerase (Lucigen, 30221) for 12 hours at 37°C followed by 2 washes each 5 min with label probe hybridization buffer (2x SSC ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were then in vitro transcribed with T7 polymerase (Lucigen). Capture SELEX was then carried out as follows ...
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bioRxiv - Immunology 2022Quote: ... rinsed once with 1x phi29 polymerase buffer (Lucigen, NxGen kit 30221) in nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using DNA QuickExtract (Lucigen), and qPCR was performed using iTaq Universal SYBR Green (Bio-rad ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was extracted (DNA extraction solution, Epicentre Biotechnologies) and edited regions were specifically amplified by PCR (primers are listed in Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from using DNA QuickExtract (Lucigen). Amplicons for indel analysis were generated by PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using DNA QuickExtract (Lucigen #QE09050). Cells were lysed in 50 μL of QuickExtract solution and incubated at 68°C for 15 min followed by 95°C for 10 min ...
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bioRxiv - Genetics 2023Quote: ... DNA was isolated using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... DNA was extracted (QuickExtract DNA extraction solution; Epicentre Biotechnologies), the region of interest was amplified by PCR (primers summarized in Table S3) ...
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bioRxiv - Genetics 2020Quote: DNA was isolated using QuickExtract DNA extract solution (Lucigen) for PCR grade DNA or the QIAamp DNA mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA extraction using QuickExtract DNA Extraction Solution (Lucigen) was performed according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... and the target exons were amplified with EconoTaq polymerase (Lucigen, Middleton, WI, USA). The resulting amplicons were subcloned using the TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNAs were prepared using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2019Quote: ... Total DNA was purified using Masterpure DNA Purification kit (Epicentre), and prepared for TnSeq by PCR amplification of transposon:genome junctions and adapter ligation following the protocol in [53] ...
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bioRxiv - Molecular Biology 2020Quote: ... cellular genomic DNA was extracted using QuickExtract DNA solution (Epicentre) and the Gaac.1826 target locus was PCR amplified and purified using DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen), depending on confluency 25 to 100 μl of solution was added to the wells containing the cells for 15 minutes at 37C ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted with QuickExtract DNA extraction solution (EpiCentre) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was isolated (Quick Extract DNA extraction solution, Lucigen), amplified by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... genomic DNA was extracted using Quickextract DNA extraction solution (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen). PCR amplified target sequence was heated to 95°C and slowly (2C/s ...
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bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated using QuickExtract DNA Extraction Solution (Epicentre). Two sets of PCR primers were designed ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) and small DNA fragments were preserved by purification with AMPure beads (Agencourt ...
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bioRxiv - Genomics 2021Quote: DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit and sequencing was performed on the Illumina NextSeq500 with 75bp single-end reads (first experiment) ...
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bioRxiv - Developmental Biology 2022Quote: ... Yolk-sac DNA was extracted (QuickExtract DNA Extraction Solution, Epicentre) and used for genotyping to distinguish heterozygous and homozygous Hand2 conditional allele ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was harvested using QuickExtract DNA Extraction Solution (Lucigen), and gDNA was prepared by heating to 65°C for 10 min followed by heat inactivation at 95°C for 2 min ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was isolated using QuickExtract DNA lysis solution (Epicentre #QE0905T), and genomic DNA flanking the targeted sequence was amplified by PCR (For1 ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was extracted with QuickExtract DNA extraction solution (EpiCentre) following manufacturer’s recommendations ...