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Citations for Lucigen :
1 - 33 of 33 citations for Beta glucuronidase GUSB E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... E.coli strains were obtained from Lucigen; Strep-Tactin resin was acquired from Iba lifesciences and Nickel resins were purchased from ThermoFisher Scientific (HisPur™ Ni-NTA resin ...
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bioRxiv - Neuroscience 2020Quote: ... transformed into E.coli TG1F+ DUO (Lucigen) with a minimal library size of 3E+08 transformants per HCDR3 length and phages were produced using VCSM13 helper phage (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... transformed into E.coli TG1F+ DUO (Lucigen) with a minimal library size of 1E+08 transformants ...
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bioRxiv - Immunology 2021Quote: ... The cloned library was expanded in Electrocompetent E.coli (Lucigen) and sequence complexity was analyzed using next generation sequencing using an Illumina MiSeq ...
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bioRxiv - Biochemistry 2019Quote: ... CLIC1 was expressed recombinantly in the C43 E.coli strain (Lucigen). The cells were lysed by sonication ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells ...
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bioRxiv - Biochemistry 2021Quote: ... CLIC1 was expressed recombinantly in the C43 E.coli strain (Lucigen). The cells were lysed by sonication ...
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bioRxiv - Microbiology 2020Quote: ... NEB Builder reaction mixtures were transformed into E.coli EPI300 cells (Lucigen) and successful integration of the S protein cDNA was confirmed by Sanger sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... the expression was conducted in the E.coli strain C41 (DE3) pLysS (Lucigen) in LBE-5052 autoinduction medium for 24 hours at room temperature ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... The resulting ligated DNA was transformed into electrocompetent cells E.coli SS320 (Lucigen) and propagated overnight.
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bioRxiv - Genomics 2019Quote: ... The ligated DNA sample was used to transform electrocompetent E.coli cells (EPI300, Epicentre), using 1 µl of ligated DNA solution per transformation ...
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bioRxiv - Biochemistry 2022Quote: ... pET15b/MBP-Vpu plasmid was transfected into E.coli BL21(DE3) competent cells (Lucigen) following the recommended by the manufacturer protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The dsDNA was purified from a 1% agarose gel and electroporated into E.coli SS320 (Lucigen) electrocompetent cells pre-infected with M13KO7 helper-phage (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells, Lucigen). 4 x 250ml LB preps (supplemented with 100 μg mL-1 carbenicillin ...
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The pDNA for IVT were isolated from the E.coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen) using the Plasmid Maxi Kit (QIAGEN) ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
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bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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bioRxiv - Biochemistry 2019Quote: The DNA constructs of Loa22 were individually transformed into expression host cell E.coli ClearColiTMBL21 (DE3) pLys (Lucigen, Middleton, WI). Loa22 and its variants were grown at 37°C in the medium with 50 μg/mL ampicillin to an OD600 of approximately 0.6 ...
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bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μg of RNA was treated with a beta version of Ribo-Zero Magnetic Gold Kit Yeast (Epicentre) to deplete rRNAs ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Plant Biology 2019Quote: Each ARF DBD was cloned in the pETite N-His SUMO Kan expression vector (Lucigen, WI) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The CDS encoding FLVA (residues 55-723) was cloned into pETite® C-His Kan Vector (Lucigen) downstream of a sequence for a Strep-tag II (WSHPQFEK ...
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bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
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bioRxiv - Microbiology 2024Quote: ... the amplified DNA was cloned into the pETite N-His vector (Expresso™ T7 cloning and expression system, Lucigen, # 49001-1), resulting in constructs with an N-terminal 6x His tag ...