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Citations for Lucigen :
1 - 50 of 56 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... mRNAs were purified after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre Biotech., WI). Fluorescently labeled cRNAs derived from these transcripts were used to probe Agilent Mouse V4.0 LncRNA Array containing probes for 22,692 mRNAs (ArrayStar ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
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bioRxiv - Immunology 2021Quote: ... and additionally purified with a mRNA-ONLY Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were maintained under regular media exchange until reaching ∼90% confluency when editing was analyzed by extracting genomic DNA using QuickExtract (Lucigen).
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA from edited and unedited HSPC samples was harvested 48h post-editing using QuickExtract DNA Extraction Solution according to manufacturer’s recommendations (Lucigen Corp., Teddington, UK) and diluted to 4.55 ng/uL in IDTE pH 8.0 (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μl Plasmid-Safe Enzyme (Lucigen), and 6 μl 10mM ATP (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... 20% End-It Enzyme Mix (Lucigen, E0025-D1), 18% Glycerol ...
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bioRxiv - Systems Biology 2020Quote: ... and 23S rRNA subunits and spaced nine bases apart were then annealed at 65 °C followed by digestion with Hybridase (Lucigen), a thermostable RNase H ...
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bioRxiv - Microbiology 2020Quote: ... and 23S subunits and spaced 9 bases apart were then annealed at 65 degrees followed by digestion with Hybridase (Lucigen), a thermostable RNAseH ...
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bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...
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bioRxiv - Cancer Biology 2023Quote: ... The melted agarose was digested with GELase enzyme (Epicentre Biotechnologies 1 unit per 50 μl of agarose suspension ...
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bioRxiv - Microbiology 2024Quote: ... FailSafe®PCR Enzyme Mix (Epicentre, Madison, WI, USA) and ScriptSeq®Index PCR Primers (Epicentre ...
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bioRxiv - Genomics 2022Quote: ... it is possible to purchase the enzyme commercially (Lucigen TNP92110) and proceed directly to adapter annealing ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified cDNA was circularized using CircLigase II enzyme (Epicentre, CL9025K), then library PCR ...
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bioRxiv - Genomics 2021Quote: ... DNase-treated RNA was subjected to terminator digestion (TEX enzyme, Epicentre) to degrade 5’-monophosphate RNA (16S and 18S ribosomal RNA) ...
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bioRxiv - Microbiology 2022Quote: ... sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre), DNase treated ...
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bioRxiv - Genomics 2023Quote: ... The eluted RNA was then treated with 5’ Terminator exonuclease enzyme (Epicentre) to remove the uncapped RNA ...
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bioRxiv - Microbiology 2020Quote: ... rRNA depletion for the mRNA library was conducted with Ribozero kit (Epicentre) with the low input protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was generated with EpiScript RT enzyme (Lucigen, 50C for 30 minutes) followed by reaction clean up with exonuclease I (Lucigen ...
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bioRxiv - Microbiology 2021Quote: ... and mRNA was enriched using the Bacterial Ribo-Zero rRNA removal kit (Epicentre). Residual mRNA was converted to sequencing libraries using TruSeq barcoded adaptors (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... The purified total mRNA was treated by 5’-Phosphate-Dependent Exonuclease (Lucigen, TER51020) to degrade RNAs with 5’ monophosphates ...
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Genomics 2021Quote: ... The libraries were amplified with 10 PCR cycles using the FailSafe PCR enzyme (Illumina/Epicentre). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Microbiology 2020Quote: ... DNAse-treated RNA (5 µg) was mRNA enriched using a Ribo-Zero Magnetic Kit (Epicentre). After Illumina sequencing the reads were mapped to C ...
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the Quantseq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... and decapped using Tobacco Acid Pyrophosphatase (TAP) enzyme (Epicentre, can be replaced by RppH from NEB) and purified by phenol/chloroform extraction ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of total RNA was treated with the RNA processing enzyme RNA 5′-polyphosphatase (Epicentre) to convert 5′-triphosphate RNA or 5′-diphosphorylated RNA to 5′-monophosphate RNA without dephosphorylating monophosphorylated RNA ...
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bioRxiv - Microbiology 2021Quote: ... TSS mapping was performed using an ExactSTAR Eukaryotic mRNA 5’- and 3’-RACE Kit (Epicentre Biotechnologies) as described in the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... Free mRNA and rRNA ends were then ligated into circular forms with a circRNA ligase (Lucigen); it favors ligation events between proximal RNA ends11 and it has limited activity at high temperature ...
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bioRxiv - Molecular Biology 2020Quote: Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on the Illumina HiSeq2500 platform (100 bp single-end reads) ...
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bioRxiv - Cancer Biology 2021Quote: ... while prokaryotic mRNA was enriched by removing rRNA by Ribo-Zero™ Magnetic Kit (Epicentre, Madison, USA). Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA (1 μg) from untreated HPAECs was digested by 2U of RNase R enzyme (Lucigen, Middleton, WI) in 10 μL reactions containing 1x digestion buffer ...
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bioRxiv - Physiology 2021Quote: ... mRNA was purified from 100 ng of total RNA by using a Ribo-Zero rRNA removal kit (Epicentre) to deplete ribosomal RNA and convert into double-stranded complementary DNA by using an NEBNext mRNA Second Strand Synthesis Module (E6111L) ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Immunology 2019Quote: 1ug of total cellular RNA was treated with RNA 5’ polyphosphatase (enzyme that converts 5’-triphosphorylated RNA into 5’-monophosphorylated RNA, Lucigen) for 30 min at 37°C or mock-treated ...
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bioRxiv - Genomics 2019Quote: ... After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme, CL9025K, Epicentre) was added to the sample and incubated for two hours at 60° C ...
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bioRxiv - Bioengineering 2020Quote: ... After mutagenesis DNA sublibrary pools were digested with appropriate restriction enzymes to remove phagemid template before transformation into SS320 electrocompetent cell (Lucigen) for phage production ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments in the range of 30-40 kbp were resolved by gel electrophoresis (2 V cm-1 overnight at 4 °C) and recovered from 1% low melting point agarose gel using GELase 50X buffer and GELase enzyme (Epicentre). Nucleic acid fragments were then ligated to the linearized CopyControl pCC2FOS vector following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were processed for directional mRNA-seq library construction using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... A 5 µg aliquot of total RNA was depleted for rRNA with the mRNA then converted into cDNA with the Scriptseq complete bacteria kit (Epicentre). The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.
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bioRxiv - Microbiology 2023Quote: ... The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4) by blunt-end ligation enzyme (Epicentre FastLinkTM kit) and ligation products in the range of 4-10 kb were extracted from a 0.7% agarose gel.
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation products were purified by removing the enzymes and transformed into 500 μl of electroporation competent TG1 cells (Lucigen, Middleton, WI) to make the library.
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bioRxiv - Genomics 2023Quote: ... About 20,000 prepared barcode beads were resuspended in 40 μl enzyme buffer (1 U/μl Fast-Link DNA Ligase (Lucigen, E0077-2-D3), 20% End-It Enzyme Mix (Lucigen ...
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bioRxiv - Genomics 2022Quote: ... and patients A and B healthy and cancer tissue RNAs (500 ng) were incubated with 20 U exonuclease I (Lucigen) and 2 U Baseline-ZERO DNase (Lucigen ...