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Citations for Lucigen :
1 - 36 of 36 citations for Adenovirus Type 40 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Wild-type proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600∼0.4 ...
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bioRxiv - Microbiology 2019Quote: ... and Type One Inhibitor (Lucigen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Type 1 restriction inhibitor (1 µl; Epicentre) was added to the plasmid DNA prior to mixing with competent cells ...
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bioRxiv - Microbiology 2022Quote: ... 40 U of RiboGuard RNase Inhibitor (Lucigen), and 1 µM of the proteins of interest ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 μL 40 U/μL RNasin (Lucigen), and 0.6 μL nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 mL 40 U/mL RNasin (Lucigen, Middleton, WI), and 0.6 mL nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Two 40 Kb mate-pair libraries were generated by Lucigen from 1 µg of gDNA using the CviQl and BfaI restriction enzymes ...
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bioRxiv - Microbiology 2021Quote: ... 40 and 50 U/μl of Ready-Lyse Lysozyme (Lucigen R1810M). qPCR was performed on the Thermo Fisher Quant Studio 3 Real-Time PCR machine with the following parameters ...
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bioRxiv - Microbiology 2020Quote: The resulting cDNA was circularized by 40 U ssDNA ligase (Lucigen) at 60 °C for 4 h ...
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bioRxiv - Molecular Biology 2020Quote: ... ssAAV vector samples were incubated with 40-U exonuclease (Lucigen Cat#E3101K) and 10-U endonuclease (Lucigen Cat#DB0715K ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA (gDNA) was extracted by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Microbiology 2022Quote: ... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
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bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction for wild type and Sts5-2A cells was performed using MasterPure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the wild type or the variants of BbZIP was expressed in the strain of C41 (DE3) pLysS (Lucigen) in LBE-5052 Autoinduction medium (30 ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... For RNAse-free IP conditions TBS-0.05% NP-40 was supplemented with 1U/ul NxGen RNAse inhibitor (Lucigen). For nuclease treated samples ...
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bioRxiv - Cell Biology 2020Quote: ... ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then ...
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bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Genomics 2019Quote: ... A fosmid library consisting of over eight million clones with a mean insert size of 40 kb was generated by Lucigen Corporation and end-sequenced on an Illumina MiSeq ...
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bioRxiv - Biochemistry 2020Quote: ... Mutant proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600 ∼0.4 ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments sized between 30 and 40 kb were isolated as previously described (Tasse et al., 2010) and cloned into pEPIFOS-5 fosmids (Epicentre Technologies). EPI100 E ...
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bioRxiv - Genomics 2023Quote: ... About 20,000 prepared barcode beads were resuspended in 40 μl enzyme buffer (1 U/μl Fast-Link DNA Ligase (Lucigen, E0077-2-D3), 20% End-It Enzyme Mix (Lucigen ...
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... Fractions containing few endogenous bacterial proteins were pooled and treated with SUMO Express Protease (Lucigen, WI) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The samples were chilled on ice for 5 minutes and 175 μl of MPC protein precipitation solution (Lucigen, USA) was added to 300 μl of the lysed sample and vortexed vigorously for 10 seconds ...
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bioRxiv - Molecular Biology 2019Quote: Proteinase K sensitivity experiments were performed using 1.5ug (protein) of gradient-purified EVs treated with 5 ug /mL Proteinase K (Epicentre) in the absence or presence of 0.05% Triton X-100 at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Selected clones lacking the expression of protein-of-interest were sequenced to confirm the knockout: cells were resuspended in QuickExtract (Lucigen), incubated at 65 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI). Remaining steps followed the recommended PureLink Genomic DNA Mini Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI). Remaining steps followed the recommended PureLink Genomic DNA Mini Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... eDNA was purified from the samples by removing the proteins and RNA using MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), and the DNA concentration was measured using NanoVue Plus (GE Healthcare ...