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Citations for Lucigen :
1 - 50 of 139 citations for 3 Phenoxybenzoic Acid Unlabeled 100 Ug Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K) for 45 minutes at RT with light shaking ...
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bioRxiv - Molecular Biology 2019Quote: Proteinase K sensitivity experiments were performed using 1.5ug (protein) of gradient-purified EVs treated with 5 ug /mL Proteinase K (Epicentre) in the absence or presence of 0.05% Triton X-100 at 37°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... with 100 U/ml of OmniCleave nuclease (Epicentre) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg/mL cycloheximide) and incubated with 15 U RNase I (Lucigen) for 45 minutes while rotating at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 8-well strips containing 3 μL of cell collection buffer (0.1% Triton X-100, 0.2 U/μL RNAse inhibitor (Lucigen)) ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco Acid Pyrophosphatase (Epicentre, discontinued) or recombinant PIR-1 was used to dephosphorylate ppp-RNAs for cloning ppp-RNAs when needed while no such treatment was required for cloning p-RNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco acid pyrophosphatase (TAP, Epicentre) was added to convert 5′ PPP or capped RNAs to 5′ P RNAs ...
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bioRxiv - Genomics 2022Quote: ... and diluted at 1:100 ratio into 250 ml LB supplemented with kanamycin (50 μg/mL) and 1x copy number induction solution (Lucigen, CCIS125). Payload DNA was isolated from E ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
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bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... The 3′ ligation oligo (listed in Supplemental Table S6) was ligated onto the cDNA using CircLigase I (Lucigen #CL4111K, 100 units) with CircLigase Reaction Buffer ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Genomics 2021Quote: ... About 30 ug of total gDNA was recovered from using MasterPure™ Complete DNA and RNA Purification kit (Lucigen), and 0.5ug-1ug gDNA was checked on a 1% agarose gel for integrity and quality ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from 3 mL of turbid liquid culture with the MasterPureTM Gram Positive DNA Purification Kit (Lucigen). DNA was quantified by using a NanodropTM device (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were incubated with Tobacco Acid Pyrophosphatase (Epicentre) and washed once with wash buffer ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Free nucleic acids were digested using OmniCleave Endonuclease (Lucigen, USA) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Purified RNAs were then treated with TAP (Tobacco Acid Pyrophosphatase, Epicentre) to convert 5′ ends of RNA to monophosphate ...
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bioRxiv - Microbiology 2019Quote: ... DNase-treated RNA was treated with Tobacco Acid Pyrophosphate (TAP) (Epicentre) at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were extracted using MasterPure yeast DNA purification kit (Epicentre, MPY80200) according the manufacturer’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... The pilT gene and flanking sequence from the FA1090 chromosome was amplified by PCR with primers PilT-F 5’-CATTGAGGTCGGCAAGCAGC-3’ and PilT-R 5’-GCATCTTTACCCAGCGCGAAAT-3’ and cloned into pSMART HC Kan (Lucigen). The cloning mix was transformed into TOP10 E ...
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bioRxiv - Molecular Biology 2022Quote: ... The 3’end of resultant cDNAs were ligated to a ssDNA linker (5’-PhosNNNAGATCGGAAGAGCGTCGTGTAG-/3SpC3/3’) using Circligase ssDNA Ligase (Epicentre) at 65°C for 12 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... purified virus RNA was treated with 5 units of tobacco acid pyrophosphatase (Epicentre) to generate 5’ monophosphorylated termini ...
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bioRxiv - Microbiology 2021Quote: ... 3-5 μl Hybridase™ Thermostable RNase H (Lucigen) and 7 μl 10x RNase H buffer preheated to 45°C was added ...
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bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted using the Epicentre MasterPure nucleic acid extraction kit (Epicentre, Madison, WI) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Library #3 was amplified using pyrophage polymerase (Lucigen, Middleton, WI).
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bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 mL 40 U/mL RNasin (Lucigen, Middleton, WI), and 0.6 mL nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Molecular Biology 2019Quote: ... and decapped using Tobacco Acid Pyrophosphatase (TAP) enzyme (Epicentre, can be replaced by RppH from NEB) and purified by phenol/chloroform extraction ...
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bioRxiv - Cell Biology 2021Quote: ... Fungal genomic acid DNA was isolated with the MasterPureTM Yeast DNA Purification Kit (Lucigen, Wisconsin, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: DNA was extracted 3 dpt using QuickExtract DNA Extraction Solution (Lucigen) and heated at 65□ for 20 min followed by 95□ for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated with 100 μL QuickExtract solution (Lucigen CorporationSupplier Diversity Partner QuickExtract DNA Extraction Solution 1.0 ...
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bioRxiv - Microbiology 2024Quote: ... 100U/ml Omnicleave (Epicentre), and Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... 100U/ml Omnicleave (Epicentre), and Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to tubes containing 3 μL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 h of removal from the incubator and within 4 h of removal from the animals.
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bioRxiv - Biochemistry 2019Quote: ... the dissolved cDNA was mixed with 3 μl of 10x CircLigase Buffer (Epicentre), 1.5 μl of 50 mM MnCl2 ...
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bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... and the 5’P of capped molecules was exposed by treatment with 5 units of Tobacco Acid Pyrophosphatase (Epicentre). RNA samples were ligated with the TIF-seq DNA/ RNA 5oligo cap using T4 RNA ligase 1 (NEB) ...
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bioRxiv - Biophysics 2021Quote: ... L and Neg sorted pools using 100 μL QuickExtract (Lucigen) per 1 million cells ...
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bioRxiv - Genomics 2020Quote: ... and then lysed in 100 μl QuickExtract™ Buffer (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was circularized with 100 U CircLigase ssDNA Ligase (Lucigen) in the presence of 1X CircLigase Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... to decap linear RNA and then was degraded using Terminator 5’-3’ exonuclease (Lucigen). The resulting RNA was put through a size selection step to remove ≤200 nts RNA using SPRI paramagnetic beads (Beckman Coulter ...