Labshake search
Citations for Lucigen :
101 - 150 of 227 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... transposable element insertional mutagenesis using EZ-Tn5™
2> Insertion Kit (Epicentre) and sequencing were performed ... -
bioRxiv - Cancer Biology 2024Quote: 2 μg of total RNA were treated with 4U of RNase R (Lucigen) at 37°C for 5 or 10 minutes in case of downregulated and at 37°C for 30 minutes for upregulated circRNAs followed by 20 minutes at 65°C ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Microbiology 2019Quote: ... Transposome mixtures were prepared by mixing the EZ-Tn5
2> transposon from Epicentre Biotechnologies ... -
bioRxiv - Evolutionary Biology 2021Quote: ... sample 2 was treated with 20 U RNase R (Epicentre/Illumina, Cat. No. RNR07250) for 1 h at 37°C to degrade linear RNAs ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage of around 200x.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Genetics 2021Quote: ... The DNA:RNA hybrids were digested with 10 U Thermostable RNase H (Epicentre, H39500) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA samples (∼2 µg) were end-repaired using the End-it kit from Epicentre Technologies (Madison ...
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bioRxiv - Microbiology 2021Quote: ... the assembled plasmid pool was used to transform 25μl of electrocompetent bacteria (Lucigen #60242-2) on a Bio-Rad Gene-Pulser 2 electroporation system (Bio-Rad # 1652105 ...
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bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
2> Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Cancer Biology 2022Quote: ... The library was transformed into electrocompetent Lucigen Endura™ Escherichia coli (Lucigen; cat. 60242-2) using a Bio-Rad MicroPulser Electroporator (#1652100) ...
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bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
2>TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
2> Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...
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bioRxiv - Developmental Biology 2021Quote: The cell pellets of picked colonies were resuspended in 10 µl QuickExtract (Epicentre, QE0905T) and the suspension was incubated at 65 °C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: Genomic DNA was extracted with 10-13 µl of QuickExtract DNA Extraction Solution (Epicentre). Fragments around the intended mutation sites were amplified by PCR in 50 µl reactions with Taq DNA polymerase (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA were digested with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... in 10 μL PCR reactions consisting of 5μL of 2X EconoTaq Master mix (Lucigen), 1uL of each primer (10μM) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 2 µl water and subjected to electroporation into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2019Quote: ... One μl of the EZ-Tn5
2> Tnp transposome complex (Epicentre BioTechnologies, Madison, WI, USA) was added to electrocompetent S ... -
bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
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bioRxiv - Microbiology 2024Quote: A transposon mutant library in pJBG007 was made using EZ-Tn5
2> Insertion Kit (Lucigen). The following components were used for a 10µl reaction (EZ-Tn5 10X Reaction Buffer (1µl ... -
bioRxiv - Cancer Biology 2023Quote: ... the pBA439_UMI20 library was electroporated into 25 μl of Lucigen Endura ElectroCompetent Cells (Lucigen, 60242-2) using prechilled 1 mm electroporation cuvettes (BioRad ...
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bioRxiv - Microbiology 2023Quote: Transposon library of Salmonella was generated using EZ-Tn5
2>Tnp Transposome kit (Lucigen, TSM99K2) following manufacturer’s instructions ... -
bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: Transposon mutagenesis was performed by electroporation using the EZ-Tn5
2> Tnp Transposome kit (Lucigen). Briefly ... -
bioRxiv - Molecular Biology 2019Quote: ... The lysate containing 10 µg RNA was treated with 20 U of RNase I (Lucigen) for 45 min at 25ºC ...
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bioRxiv - Microbiology 2021Quote: ... To extract DNA 10 ul of culture was added to 500 ul QuickExtract Solution (Epicentre) and treated 65 °C for 6 min followed by 98 °C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... were digested overnight with 10 units of Plasmid-Safe ATP-Dependent DNAse (Lucigen, Middleton, Wisconsin) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... gRNAs were degraded by the addition of 0.5 µl of RNaseI (10 U/µl, Epicentre) and incubation at 37°C for 10 min ...
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bioRxiv - Genomics 2021Quote: ... The libraries were amplified with 10 PCR cycles using the FailSafe PCR enzyme (Illumina/Epicentre). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
2> Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Microbiology 2021Quote: ... 100µl of competent cells was mixed with 1 µl of EZ-Tn5 KAN-2 Tnp Transposome (Epicentre) and electroporated at 1800V ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... were used to amplify lnt that had been disrupted by the insertion of Tn5
2> (Epicentre) [6] ... -
bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
2> Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Neuroscience 2021Quote: ... We used 1.5 μL of the ligation mix and electroporated 30 μL competent bacteria (Lucigen, 60242-2) with ...
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bioRxiv - Genetics 2020Quote: ... 2 µl per tube was transformed into two tubes of 50 µl of electrocompetent cells (Lucigen DUO) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA of 2 mL overnight culture was extracted with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and whole PCR Tag analysis was performed on population level to test the general presence of all tRNAs within the population (data not shown) ...
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bioRxiv - Molecular Biology 2023Quote: ... and excess unligated linker was removed using 10 U/µl 5’ deadenylase/RecJ exonuclease (Epicentre, RJ411250), followed by pooling and purification of ligated footprints using an Oligo Clean and Concentrator column (Zymo Research ...
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bioRxiv - Microbiology 2019Quote: ... The cells in glycerol were electroporated with the EZ-Tn5 R6Kγori/KAN-2>Tnp transposon (Epicentre, Madison WI) using an Eppendorf Electroporator 2510 at 2.5 kV yielding around 5 ms ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
2> Tnp Transposome (Epicentre) in 50 μL of EV18-pkD46 electrocompetent cells ...