Labshake search
Citations for Lucigen :
601 - 650 of 880 citations for 2'3' Cyclic GAMP cGAMP ELISA Kit 1 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted from yeast pellets using the MasterPure™ Yeast RNA Purification Kit (Lucigen Cat. No. MPY03100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
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bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
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bioRxiv - Bioengineering 2023Quote: ... a PCR-amplified NLuc gene block was transcribed using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, ASF-3507) following manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was isolated from the thawed cell pellets using the MasterPure Yeast DNA Purification kit (Lucigen MPY80200). The DNA was digested with XhoI and NgoMIV and fractionated on a 0.6% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of 6 mL of recovery medium (Lucigen, F98226-1) and incubation at 37 °C for one hour at 280 rpm rotation ...
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bioRxiv - Synthetic Biology 2021Quote: ... a highly competent derivative of DH10β was obtained from Lucigen (60107-1)53 ...
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bioRxiv - Neuroscience 2022Quote: ... together with 1 1l genomic DNA in QuickExtract DNA Extraction Solution (Lucigen). After droplet generation with the QX200 Droplet generator (Biorad) ...
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bioRxiv - Biochemistry 2022Quote: ... The electroporated bacteria were then cultured in recovery medium (Lucigen 80026-1) for 1 hour ...
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bioRxiv - Genetics 2019Quote: ... 1 μg of RNA was treated with 20 units of RNAseR (Epicentre) in a 25μl reaction at 37°C for 30 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Genetics 2023Quote: ... Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen, 60242-1) via electrotransformation (Bio-Rad Gene Pulser II) ...
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bioRxiv - Cell Biology 2023Quote: ... Ruptured cells were treated with 1 U/μl OmniCleave endonuclease (Lucigen, OC7850K) for 5 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Cell Biology 2019Quote: ... were grown overnight at 37°C with 12.5 µg of chloramphenicol and extracted using a BACMAX DNA purification kit (Epicentre). Then ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
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bioRxiv - Immunology 2021Quote: ... libraries were constructed from first strand cDNA using ScriptSeqTM v2 RNA-Seq library preparation kit (Epicentre Biotechnologies, Madison, WI). Briefly ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a (modified) CTAB protocol or a rapid desalting method (MasterPure™ Complete DNA and RNA Purification Kit; Lucigen Corporation).
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bioRxiv - Genomics 2020Quote: ... an aliquot of 25 uL was reserved from each sample for extraction of DNA with MasterPure™ kit (Epicentre) and bacterial cell count was measured using qPCR (TaqMan™) ...
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bioRxiv - Genomics 2020Quote: ... gDNA for the rest of the strains was extracted using the Masterpure Complete DNA and RNA purification kit (Lucigen) using the protocol for tissue samples ...
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bioRxiv - Molecular Biology 2019Quote: ... and shSmt3 GLKD lines was depleted of ribosomal RNA with the Ribo-Zero™ rRNA Removal Kit (Epicentre/Illumina). Initial RNA-seq libraries were made using the TruSeq RNA prep kit by Illumina (shW ...
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bioRxiv - Microbiology 2019Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... About 30 ug of total gDNA was recovered from using MasterPure™ Complete DNA and RNA Purification kit (Lucigen), and 0.5ug-1ug gDNA was checked on a 1% agarose gel for integrity and quality ...
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Barcoding and demultiplexing Oxford Nanopore native RNA sequencing reads with deep residual learningbioRxiv - Genomics 2019Quote: ... Curlcake in vitro transcribed (IVT) sequences were produced using the Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). Sequin IVT sequences were produced using SP6 Polymerase (NEB-M0207S) ...
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bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Phage DNA extractions were performed from high titer suspensions using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 20pg of in vitro transcribed RNA synthesized from the T7 control DNA Template (AmpliScribe™ T7 Transcription Kit, Epicentre) was added to the samples ...
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bioRxiv - Molecular Biology 2020Quote: The initial RNA library was transcribed from 1µg of input dsDNA using the AmpliScribe T7-Flash Transcription Kit (Epicentre). 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI) with a total amount of 2 ug RNA as an input for each library ...
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Epigenetics of post-operative delirium: A genome-wide DNA methylation study of neurosurgery patientsbioRxiv - Neuroscience 2022Quote: Genomic DNA was extracted from whole blood tissues with the MasterPure DNA extraction kit (MCD85201, Epicentre, Madison, WI, USA) following the recommended protocol ...
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bioRxiv - Genomics 2022Quote: DNA was isolated from the 16 parental strains using the Masterpure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). The Pacific Biosciences protocol “Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” was used to create libraries from 30 micrograms of DNA ...
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bioRxiv - Genomics 2022Quote: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from 50mL flash-frozen cell pellets grown to mid-log (OD600 ∼0.65-0.85) using MasterPure™ yeast RNA purification kit (Lucigen) including a DNaseI treatment step ...
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bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed on a Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was subjected to ribosomal RNA depletion using the Ribo-Zero Magnetic Gold Kit (Human/Mouse/Rat; Epicentre), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Total RNAs of nodule and root were processed using the Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA) to deplete ribosomal RNAs ...
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bioRxiv - Bioengineering 2023Quote: ... The genomic DNA was extracted from homogenized liver tissues using the MasterPure Complete DNA and RNA Purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Isolated DNA was used to construct a fosmid library using the CopyControl™ Fosmid Library Production kit (Lucigen Corporation) following the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: Genomic DNA extraction from homogenized liver tissue was performed using the MasterPure Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: High molecular weight DNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. Seven days old-mycelia of P ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the bacterial pellets using the MasterPure™ Complete DNA and RNA purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and used to electroporate 1-4 tubes of Endura DUO electrocompetent cells (Lucigen) at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width ...