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Citations for Agilent :
1 - 50 of 982 citations for pTH Related Protein Splice Isoform 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was generated by introducing mutations in the 3’ splice site of the pPOLI-WSN-M plasmid using QuikChange II site-directed mutagenesis protocol (Agilent). pPolI-WSN-M-M2SMRtoAAA (Mut1) ...
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bioRxiv - Cell Biology 2019Quote: Padi2 splice variants were amplified with Pfu Turbo DNA polymerase (Agilent) from cDNA using In-Fusion primers listed below ...
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bioRxiv - Bioengineering 2019Quote: ... Removal of the native splice acceptor in the 3’ homologous arm of HDR templates was achieved via site-directed mutagenesis (Agilent Technologies, 200523).
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bioRxiv - Cell Biology 2021Quote: ... Levels of splice variants were analyzed by TAPE 2200 capillary electrophoresis (Agilent) using high throughput D1000 ScreenTapes (Agilent) ...
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bioRxiv - Genetics 2020Quote: ... following antibodies and related dilutions were used: MyoD (Dako, M3512, 1:800), Pax7 (Developmental Studies Hybridoma Bank ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Cell Biology 2022Quote: ... Each mitochondrial oxygen respiration-related value was calculated using Wave software (Agilent Technologies).
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bioRxiv - Genetics 2021Quote: ... Target sequencing was performed for all the exons of 550 cancer-related genes (Agilent SureSelect XT Custom kit ...
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bioRxiv - Developmental Biology 2022Quote: Tmod isoforms in pRSF_Duet1 were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mutant M1237I spike related constructs were generated using the QuikChange II site-directed mutagenesis kit (Agilent) with the primer set of S-M1237I-F ...
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bioRxiv - Genetics 2020Quote: ... the exonic regions and flanking splice junctions of the genome were captured using the Clinical Research Exome kit (Agilent Technologies, Santa Clara, CA). Massively parallel (NextGen ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of FAM104A isoform 5 was mutated using the QuikChange XL II kit (Agilent) with primers FAM104A_NL-RR_fwd (5’-cct cta ctt cca cat ccg cca gac ccg cag gga ggc cca ctt cc) ...
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bioRxiv - Molecular Biology 2022Quote: contains the canonical human HELZ2 long isoform cDNA (2649 bps) cloned into pCMV-3Tag-9 (Agilent Technologies), which allows the expression of a HELZ2-3xMYC fusion protein ...
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bioRxiv - Genetics 2021Quote: ... Target sequencing was performed for all the exons of 550 cancer-related genes (Agilent SureSelect XT Custom kit; Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... oxygen consumption rate (OCR) and related extracellular acidification rate (ECAR) were determined in a Seahorse XFe24 analyzer (Agilent Techonoligies) by adding 10 mM glucose ...
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bioRxiv - Biochemistry 2023Quote: ... The free HSF2BP protein was analyzed using a BIOSEC 3 column (Agilent) equilibrated in 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Quality and the related size of small RNA was assessed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). 130 ng of total RNA was labeled with biotin using the FlashTag Biotin HSR RNA Labeling Kit (Applied Biosystems ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Plant Biology 2022Quote: ... Photorespiration-related organic acids other than glyoxylate were quantified using a GC-MS system (7890B/5977A; Agilent Technologies, Santa Clara, CA) equipped with a DB-5MS capillary column (30 m ...
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bioRxiv - Biochemistry 2021Quote: ... The purified S protein was separated by an SEC column (BioSEC-3, Agilent, USA) connected to an HPLC system (Analytical HPLC 1260 LC system ...
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bioRxiv - Immunology 2023Quote: ... and rabbit polyclonal antibodies against CD-3 protein (Dako, Glostrup, Denmark; cat. no. A0452).
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Biophysics 2019Quote: ... construct by deleting 14aa from near the C-terminus of PRC1 isoform 1 (aa:1-620) using Quikchange mutagenesis (Agilent). The PRC1 isoform 2 gene was then in a pET-DUET plasmid containing an N-terminal histidine tag followed by a Tobacco Etch Virus (TEV ...
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bioRxiv - Bioengineering 2019Quote: ... Then endogenous peroxidase block was performed by using 3% H2O2 in water to cancel the interference of peroxidase in the final step.[33] Protein block buffer (Dako Protein Block) was used to treat the tissue slides to mask the non-specific binding sites ...
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bioRxiv - Cell Biology 2024Quote: ... an MluI restriction site was introduced immediately upstream of the stop codon and XhoI site in pBluescript plasmids bearing full-length Slik and SlikKD coding sequences (PA isoform) as EcoRI-XhoI fragments (Hipfner and Cohen, 2003) by QuickChange mutagenesis (Agilent Technologies). For pUAST/SlikCCD-GFP ...
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation in 3% hydrogen peroxide and in serum-free protein block solution (Dako, X0909) before adding primary antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... washed in PBS (3 × 5min) and incubated with serum free protein block (Dako, Cat# X090930-2) for 1h at RT ...
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bioRxiv - Pathology 2022Quote: ... and a monoclonal antibody directed against the alpha isoform of smooth muscle actin at a working dilution of 1/100 (a-SMA, clone 1A4, n M0851; Dako, Denmark A/S). Alligator skeletal muscle was used as positive control for anti-desmin antibody (Supplemental figure 3A) ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of protein (10 mg/ml) were injected into a size-exclusion column (Agilent BioSec-3) equilibrated in 50 mM Tris ...
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bioRxiv - Physiology 2020Quote: ... 3’-3-Diamino-benzidine (DAB+, Dako) was used as substrate chromogen ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then incubated for 40 mins in 3% H2O2 TBST solution and blocked with protein block (Dako) for 20 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... The free HSF2BP protein as well as the complex between ARM and F15X were analyzed using a Bio SEC-3 column (Agilent) equilibrated in 25 mM Tris-HCl buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were developed with 3′3-diaminobenzidine (DAKO Cytomation), following the manufacturer’s instructions.
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bioRxiv - Pathology 2019Quote: ... and 3-3′-diamino-benzidine-tetrahydrochloride (DAB, Dako, K3468) revelation ...
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bioRxiv - Physiology 2021Quote: ... and 3-3′-diamino-benzidine-tetrahydrochloride (DAB, Dako, K3468) revelation.
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slides were then treated with 3% hydrogen peroxide for 10 min and then blocked with Serum-Free Protein Block (Dako, X0909) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 3% hydrogen peroxide to quench endogenous peroxidase activity and 15 minutes in serum-free protein block (DAKO, Glostrup, Denmark). Sections were then subjected to appropriate antigen retrieval methods (if needed ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... Chromagen was developed using the DAB (3, 3-diaminobenzidine) reagent (Dako). Sections were counter-stained using pre-filtered Mayer’s haematoxylin (Amber Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen–antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with haematoxylin (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with hematoxylin (Dako ...
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bioRxiv - Physiology 2022Quote: ... Antigen–antibody complexes were revealed with 3-3′- diaminobenzidine (K346811, Dako) or the DAB (Polymer ...
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bioRxiv - Immunology 2021Quote: ... and Protein block (Protein block (Dako) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Tissue sections were colored via DAB-staining (3, 3-diaminobenzidine; Dako, K3468).
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bioRxiv - Cancer Biology 2022Quote: ... 3’-Diaminobenzidine (DAB, Agilent) and then counterstained with haematoxylin (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... in 3 cycles for 3 hours using the Seahorse XFe24 analyzer system (Agilent Technologies). Inhibitors and substrates were used at the following concentrations ...
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bioRxiv - Developmental Biology 2019Quote: ... service pack 3 (Agilent Technologies).