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Citations for Agilent :
1 - 34 of 34 citations for West Nile virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Bioengineering 2023Quote: ... The obtained lysate was directly analyzed by HPLC (Agilent 1100 with a quaternary pump and diode-array detector (DAD)) ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lysate reactions were performed in 2 mL glass HPLC vials (Agilent) charged with indole (final conc ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Genomics 2020Quote: ... Lysates and positive controls (Human Universal Reference RNA - uhrRNA (Agilent Cat# 740000), and Human Brain Total RNA brRNA (ThermoFisher AM7962)) ...
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bioRxiv - Microbiology 2022Quote: ... Homogenized SDS lysates were diluted 1:1 with RNA lysis buffer (Agilent) and RNA was extracted using the Absolutely RNA Microprep Kit (Agilent ...
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bioRxiv - Genomics 2021Quote: ... 15 ul of cell lysate was transferred using a Bravo (Agilent Technologies) into the plate containing RT mix ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were revealed by chemi-luminescence (Super Signal West Dura Substrate, Pierce) using a secondary peroxydase-conjugated antibody (Dako) at a dilution of 1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Cell Biology 2020Quote: The digested lysates were desalted using C18 Omix tips (Cat number A57003100, Agilent) and dried down in 50 μg portions ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Molecular Biology 2023Quote: ... 100 µL per sample of PNA-annealed lysate was then run through an HPLC (Agilent) with a DNAPac PA100 anion-exchange column (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized to urea lysate protein levels and analyzed using Wave Desktop Software (Seahorse Bioscience), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DA content in lysates was measured using high-performance liquid chromatography (HPLC) equipped with fluorometric detection (Agilent) and a Zorbax Eclipse Plus C18 column (125 × 4.6 mm ...
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bioRxiv - Systems Biology 2019Quote: All lysates were subject to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described (Havugimana et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Systems Biology 2020Quote: Treated and control cell lysates were subjected to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described24 ...
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bioRxiv - Microbiology 2021Quote: ... gondii cell lysate and enriched eluates from affinity beads using ion- exchange (IEX) liquid chromatography by an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) individually ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...