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Citations for Agilent :
1 - 50 of 1303 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The cells were fixed with 100 ml chilled methanol and incubated with Peroxidase Block (Dako) followed by mouse anti-CMV IE-1/IE2 mAb (Chemicon ...
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bioRxiv - Microbiology 2021Quote: ... Incorporation of 13C was quantitated and corrected for natural 13C abundance using Profinder B.08.00 (Agilent Technologies). LC-MS data was deposited in the MetaboLights database47 under the accession code MTBLS2374 (www.ebi.ac.uk/metabolights/MTBLS2374).
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bioRxiv - Microbiology 2020Quote: ... Quantitative detections of 15N2 and 15NO were performed by GC/MS (model 7890A/5975C, Agilent) equipped with a CP-Molsieve 5A Plot (25 m×0.32 mm×30 μm ...
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bioRxiv - Immunology 2023Quote: ... and 400µl of ice-cold LC-MS grade methanol (Infinity Lab Ultrapure LC-MS Methanol, Agilent technologies; 5191-4497) was added to each well and allowed to sit for 5 mins ...
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bioRxiv - Synthetic Biology 2023Quote: ... NMR spectroscopic data (1H, 13C, HSQC, COSY, and HMBC) were collected by Agilent 600 MHz (14.1 Tesla ...
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bioRxiv - Pathology 2021Quote: ... 3′-diaminobenzidine tetra-hydrochloride chromogen (DAB, K3468, Dako) was added to all sections and the reaction was stopped with distilled water ...
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bioRxiv - Biochemistry 2021Quote: ... All 13C NMR spectra were obtained with a Varian VNMRS 600 MHz NMR (Agilent) spectrometer equipped with a 3 mm standard probe ...
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bioRxiv - Plant Biology 2022Quote: ... Compounds were then further purified to ≥98% purity by HPLC (Agilent 1100 Series) equipped with a diode array UV detector ...
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bioRxiv - Neuroscience 2021Quote: ... 25 and 50 ppb with Sc-45 (ICP-MS internal standard mix 1 ug/mL in 2% HNO3, Agilent Technologies) as the internal standard for Cu-63.
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bioRxiv - Physiology 2022Quote: ... [U-13C]-palmitate enrichment and [1,1,2,3,3-2H]-glycerol enrichment were measured by GC-MS/MS (Agilent GC model 7890A and Waters Quattro Micro) ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Biochemistry 2023Quote: ... B: 98% acetonitrile/0.1% FA in water) (1290 Infinity II LC system, Agilent Technologies, Waldbronn, Germany). The temperature within the customized LC system was kept at 0 °C to minimize the back exchange ...
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bioRxiv - Microbiology 2021Quote: ... Two ug of tryptic digested Mars-14 (Agilent, Santa Clara, CA) depleted serum were eluted from a high-capacity nano-HPLC Chip (160 nL ...
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bioRxiv - Microbiology 2023Quote: ... The produced 13C-CO2 was measured directly from the exetainer headspace with a GC-MS (Agilent 5975C inert MSD). Liquid 13CO2 concentrations were calculated with the Henry coefficient (Supplementary Infomration) ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... Methanol was measured with a gas chromatograph (Agilent Technologies, model 7890B, CA, USA) with an analytical column (DB-WAX ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice and incubated with LPS (100 ng/mL) in XF based medium (Agilent, 103334-100) supplemented with 4.5 g/L glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
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bioRxiv - Microbiology 2022Quote: ... the increase of produced 13C-CO2 was measured directly from the headspace with a GC-MS (Agilent 5975C inert MSD). In addition ...
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bioRxiv - Systems Biology 2020Quote: ... and 100 μg/mL sonicated salmon sperm DNA (Agilent 201190-81) in 2X SSC ...
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bioRxiv - Cancer Biology 2019Quote: ... For antigen retrieval the slides were incubated for 30 min at 98 °C in 1x Target retrieval solution (Dako). Sections were stained by adding primary antibodies diluted in immunofluorescence buffer (IFB ...
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bioRxiv - Microbiology 2020Quote: ... sections underwent antigen retrieval in citrate buffer (pH 6.0, 20 min at 98°C) followed by blocking of endogenous peroxidase (peroxidase block, S2023, Dako) for 10 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using Dako Citrate buffer (pH 6.0) for 30 minutes at 98°C in a PT Link rinse station (Dako). Tissue sections were blocked in PBTx containing 5% BSA (Merck ...
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bioRxiv - Immunology 2019Quote: ... as internal standard were analyzed by 1D 1H and 1H{13C}-HSQC NMR on a 14.1 T DD2 NMR spectrometer (Agilent Technologies, CA). 1D 1H spectra were acquired using the standard PRESAT pulse sequence with 512 transients ...
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bioRxiv - Immunology 2020Quote: ... succinate and malate and their corresponding 13C labeled counterparts were extracted and deconvoluted using MassHunter software (Agilent Technologies, New Castle, DE). Retention time consistency was manually rechecked and compared to authentic compounds that were injected under similar chromatographic conditions ...
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bioRxiv - Plant Biology 2023Quote: ... an anion-exchange cartridge (Bond Elut DEA 100 mg/1 mL, Agilent), and a silica cartridge (Sep-Pak Silica 1 cc Vac Cartridge ...
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinization and antigen retrieval was performed in the low pH EnVision FLEX Target Retrieval Solution at 98 °C for 24 minutes in the DAKO PT link from Agilent. Antibodies were diluted in DaVinci Green Diluent (Biocare ...
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bioRxiv - Immunology 2020Quote: ... B: Acetonitrile: Methanol − 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Molecular Biology 2020Quote: ... Trans-zeatin was extracted with 80% methanol from samples and analyzed using HPLC (Agilent 1100) connected to an UV detector (λ = 274 nm) ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated in methanol-validated polyclonal rabbit anti-GFAP IgG (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:1000 in PBS/Triton-X/donkey serum/DMSO solution for 4 days at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... B: Acetonitrile: Methanol – 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were re-dissolved in 100 µL solvent A (0.1% TFA in water/ACN (98:2, v/v)) and injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Microbiology 2019Quote: ... The dried residues were then resuspended in 500 μL methanol and analyzed by HPLC-MS (Agilent Single Quad ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Immunology 2020Quote: ... Generated data were normalized to viable cell number in million/ml at the viability was found to be consistently between 96% and 99% as determined by trypan blue exclusion assay (ViCell, Beckmann Coulter) and analyzed using Seahorse Glycolysis Stress Test Generator (Agilent).
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear magnetic resonance (NMR) spectra including the 1H and 13C NMR spectra were recorded on a Varian Mercury Plus 400 NMR spectrometer (Agilent Technologies, Santa Clara, CA). Proton chemical shifts are reported as parts per million (δ ppm ...
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bioRxiv - Physiology 2023Quote: ... and the mitochondria are resuspended in 1 mL of Seahorse medium (Agilent #103335-100) with supplemented 10 µL of 5 mM pyruvate (Sigma #P2256-100G ...
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bioRxiv - Physiology 2023Quote: ... and the mitochondria are resuspended in 1 mL of Seahorse medium (Agilent #103335–100) with supplemented 5 mM pyruvate (Sigma #P2256-100G) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting extract was dissolved in methanol (200 μL) before being analysed by HPLC (Agilent 1290 UHPLC). To verify peak identity a representative set of samples were analysed by LCMS (Shimadzu IT-ToF LCMS platform) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: E-cigarette liquids were diluted 100X into spectral grade methanol and injected into the GCMS detector (Agilent 7890A gas chromatograph with 5975 MSD detector) ...
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bioRxiv - Biophysics 2021Quote: ... pH 5.9) and buffer B (75 % (v/v) buffer A with 25 % (v/v) Methanol) (Agilent 1200). A different concentration gradient of c-di-AMP and AMP were used to prepare a standard curve for c-di-AMP and AMP ...
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bioRxiv - Immunology 2022Quote: ... Solid phase extraction was performed on methanol and sodium acetate conditioned Bond Elut Certify II cartridges (Agilent). After loading the samples ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Cell Biology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...
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bioRxiv - Physiology 2023Quote: ... freshly prepared) was flowed at 1 ml/min through a 1:100 flow splitter (Agilent Technologies 1260 Infinity II ...