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Citations for Agilent :
1 - 50 of 1245 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike mutations were introduced using the QuikChange II XL site-directed mutagenesis protocol (Stratagene). The presence of the desired mutations was determined by automated DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: SpyCatcher003-mi3 nanoparticles (78) were expressed in BL21 (DE3)-RIPL E coli (Agilent) transformed with the pET28a His6-SpyCatcher003-mi3 gene (Addgene ...
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Immunology 2021Quote: SpyCatcher003-mi3 particles were prepared by purification from BL21 (DE3)-RIPL E coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (including an N-terminal 6x-His tag ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Microbiology 2020Quote: ... A series of alanine mutants were introduced into the SARS-CoV-2 spike protein using the QuickChange mutagenesis kit or the QuickChange multi-mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Microbiology 2023Quote: Site-directed mutagenesis of SARS-CoV-2 spike was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent 200522), using primers listed in Supplementary table S2 and according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (Patil et al. ...
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Systems Biology 2022Quote: The DNA integrity and long-term stability were evaluated by Agilent 2200 TapeStation system (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Systems Biology 2019Quote: ... and Spike Kit (Agilent, UK). cDNAs were hybridized to Mouse miRNA Microarray ...
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) were chemically transformed with 1:4 diluted Gibson assembly products and plated onto ampicillin-agarose plates ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Agilent) were used for cloning other plasmids ...
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bioRxiv - Genomics 2019Quote: ... coli (Agilent). After cultures reached an OD600 nm of 0.5 ...
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bioRxiv - Immunology 2021Quote: ... coli (Agilent) were transformed with pET-6xHis-SUMO bacterial expression vectors for full-length ORF9 or C-terminally truncated ORF9 1–244 (ORF9-N) ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) as previously described (57) ...
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bioRxiv - Microbiology 2021Quote: ... coli (Agilent), grown as starter cultures in MDG media (0.5% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Stratagene) using glutathione (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) grown in high salt Luria broth (Sigma ...
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bioRxiv - Immunology 2020Quote: ... coli (Agilent), and purified with glutathione sepharose (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... coli (Agilent). Dilutions of 1:1000 and 1:10,000 were plated on LB agar plates supplemented with 100 μg/mL ampicillin to estimate the number of unique transformants in each library ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli (Agilent) and colonies were grown on MDG agar plates (1.5% agar ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (89 ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with pET15b p97 ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent). Bacterial cell pellets were lysed using a cell disruptor in the presence of 2.0 mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with the UBXD1 expression vectors and used for large-scale expression ...
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bioRxiv - Genomics 2023Quote: ... coli (Stratagene) for protein production using Isopropyl β-d-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent). Uniformly labeled (U-13C,15N ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Agilent) were transformed with a vector containing sequences encoding for GELYTACs or nanobody only controls with pelB signal sequence for localization to the periplasm for disulfide bond formation ...
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bioRxiv - Microbiology 2024Quote: ... coli (Agilent), colonies were grown on 1.5% agar MDG plates (2 mM MgSO4 ...
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bioRxiv - Biophysics 2023Quote: ... and E-cadherin (E-cadherin Monoclonal Mouse clone NCH-38, DAKO), in blocking solution (TBST ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... coli (Agilent #200314). Colonies were miniprepped and Sanger sequenced using a set of primers to ensure proper assembly and in-frame V regions (Supplementary Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Agilent). Cells were grown to optical density at 600nm (OD600 ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent technologies). Small-scale expression was done in 5 ml of auto-inducible terrific broth media (ForMedium ...
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bioRxiv - Physiology 2019Quote: ... coli (Agilent Technologies). 30 ml bacterial starter cultures were grown at 37° C overnight and then added to 1.5 liters of LB media at 37° C ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (Stratagene) with an N-terminal TEV cleavable GST tag ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Agilent) were transformed with pET28-S plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Agilent) were transformed with ~2 μg αHL-D8H6 plasmid40 without heat shock and incubated on LB-agar plates containing 25 g L−1 lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2022Quote: ... coli strain (Stratagene). Cells were grown at room temperature in 2L of standard LB media containing kanamycin and chloramphenicol to an OD600 of ∼0.6 ...