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Citations for Agilent :
1 - 50 of 1617 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike mutations were introduced using the QuikChange II XL site-directed mutagenesis protocol (Stratagene). The presence of the desired mutations was determined by automated DNA sequencing ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Microbiology 2020Quote: ... A series of alanine mutants were introduced into the SARS-CoV-2 spike protein using the QuickChange mutagenesis kit or the QuickChange multi-mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
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bioRxiv - Microbiology 2023Quote: Site-directed mutagenesis of SARS-CoV-2 spike was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent 200522), using primers listed in Supplementary table S2 and according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (Patil et al. ...
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bioRxiv - Biophysics 2022Quote: Point substitutions within RBD in SARS-CoV-2 spike gene were introduced by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies Inc.) following the manufacturer’s protocol and by overlapping PCR strategy as described previously (33) ...
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bioRxiv - Cell Biology 2023Quote: ... Horseradish Peroxidase-conjugated Goat Anti-Rabbit (Dako P044801-2) and Rabbit Anti-Mouse (Dako P044701-5 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 pseudovirus was produced using HEK293T cells transfected with GeneJammer (Agilent) using IgE-SARS-CoV-2 spike plasmid (Genscript ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-IgG horseradish peroxidase (Dako) and chemiluminescent detection (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-IgG horseradish peroxidase (Dako) and chemiluminescent detection (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... followed by streptavidin horseradish peroxidase (Dako) 1:300 in the same blocking solution for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... followed horseradish peroxidase-conjugated streptavidin (Dako) and 3-Amino-9-ethylcarbazole substrate ...
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bioRxiv - Immunology 2020Quote: ... The RBD domain of SARS-CoV-2 S was biotinylated and tetramerized with streptavidin-APC (Agilent). The APC decoy reagent was generated by conjugating SA-APC to Dylight 755 using a DyLight 755 antibody labeling kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... followed by EnVision FLEX DAB+ Chromogen in Substrate buffer (Agilent; anti-SARS-CoV-2, -CD8, -CD45R, -Iba1) for 10 min at RT or the DAB-Map-Kit (Ventana ...
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bioRxiv - Cancer Biology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies (DAKO) were used for visualization using chemiluminescence (Lumi-Light ...
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bioRxiv - Cell Biology 2022Quote: ... α-rabbit horseradish peroxidase conjugate (1:2000, Dako). When multiple antibodies of the same origin were used ...
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bioRxiv - Cell Biology 2019Quote: ... Horseradish peroxidase-conjugated anti-mouse (Dako, P0260) or anti-rabbit (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... The horseradish peroxidase-streptavidin system (Dako, USA) was applied for IHC signal detection ...
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bioRxiv - Cancer Biology 2021Quote: ... EnVision Flex/HRP (Horseradish peroxidase) (Dako-Agilent) was used for signal amplification for 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... EnVision Flex/HRP (Horseradish peroxidase) (Dako-Agilent) was used for signal amplification for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Biochemistry 2020Quote: ... IgG horseradish peroxidase (HRP)-conjugated antibody (DAKO P0447) was diluted 1/1000 in blocking solution and added to the wells ...
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bioRxiv - Biochemistry 2021Quote: ... IgG horseradish peroxidase (HRP)-conjugated antibody (DAKO P0447) was diluted 1/1000 in blocking solution and added to the wells ...
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bioRxiv - Cancer Biology 2020Quote: ... stained with horseradish peroxidase-conjugated secondary antibodies (DAKO) and bands detected using ECL Plus (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Horseradish peroxidase-conjugated secondary antibodies (1:2500, DAKO) were used and visualized with chemiluminescence (Lumi-Light ...
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bioRxiv - Biophysics 2022Quote: ... Secondary horseradish peroxidase conjugated antibodies were from Dako Cytomation ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Cell Biology 2021Quote: ... The SARS CoV-1 3aY109A mutant was generated by QuikChange mutagenesis (Stratagene) using the forward primer 5’-GCGCAATTTTTGTACCTGGCGGCCTTGATATATTTTC-3’ and the complementary reverse primer ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...
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bioRxiv - Genomics 2021Quote: ... the initial concentrations of purified SARS-CoV-2 and the universal human reference RNA (UHRR, Agilent Technologies, product number 740000) were determined by ddPCR as described above ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Microbiology 2023Quote: ... were prepared and stained with hematoxylin eosin (HE) for histological examination or subjected to immunohistological staining to detect SARS-CoV-2 antigen (performed in an autostainer; Agilent), using the horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase-conjugated anti-mouse (Dako, #P0447; 1:4,000) or anti-rabbit IgG (gamma-chain specific ...
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bioRxiv - Molecular Biology 2021Quote: ... Horseradish Peroxidase conjuguated anti-mouse IgG (Dako, 1/5000) and anti-rabbit IgG (Dako ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies conjugated to horseradish peroxidase (DAKO, Glostrup, Denmark) were incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and horseradish peroxidase-conjugated secondary antisera (Dako, Glostrup, Denmark) for TSA were used ...
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bioRxiv - Cell Biology 2022Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse IgG, Dako, P0260 ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated immunoglobulins were obtained from Agilent Technologies Denmark ...
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bioRxiv - Immunology 2020Quote: ... horseradish peroxidase (HRP)-labelled anti-rabbit antibody (Dako K4003) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies (DAKO, 1:2000) were used for visualization using chemiluminescence (Lumi-Light ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a streptavidin-linked horseradish peroxidase (SM802, Dako) for 20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... either the horseradish peroxidase-conjugated rabbit anti-mouse (DAKO) or the horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... horseradish peroxidase (HRP)-conjugated secondary antibodies were from DAKO. Y27632 was from Calbiochem ...
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bioRxiv - Cancer Biology 2023Quote: ... we used a secondary horseradish peroxidase-labeled polymer (Dako) and AEC Substrate Chromogen (Vector Laboratories).