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Citations for Agilent :
1 - 50 of 1776 citations for Recombinant Mouse TNFRSF17 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary biotinylated goat anti-mouse antibody (Dako) was added to the slides 1/400 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... polyclonal rabbit anti-mouse IgG biotinylated and polyclonal goat anti-rabbit IgG biotinylated (Dako) (IHC ...
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bioRxiv - Cancer Biology 2021Quote: ... and Biotinylated Goat Anti-Mouse Ig (E0433, DAKO). DAB was used as chromogenic method of detection and signal was developed using the VECTASTAIN Elite ABC HRP Kit (PK-6100 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated recombinant NS1 was purified by FPLC and then tetramerized to fluorochrome-conjugated streptavidin (Prozyme). To detect NS1-specific B cells ...
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bioRxiv - Immunology 2019Quote: ... biotinylated rabbit anti-mouse IgG ([Code E0464]; Dako, Glostrup, Denmark) was added ...
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bioRxiv - Biochemistry 2021Quote: ... 2) biotinylated goat anti-mouse Ig antibody (Dako, Glostrup, Denmark), diluted 1:5000 with TBS-BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Biotinylated goat-anti-mouse (Dako, Glostrup, Denmark; E0433; 1:200) was used as secondary antibody (1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated anti-mouse/rabbit secondary antibodies and Streptavidin-HRP (Dako), followed by TSA visualization with fluorophores Opal 520 ...
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bioRxiv - Molecular Biology 2021Quote: ... and corresponding biotinylated goat-anti-mouse secondary antibody (Dako, Hamburg, Germany). Thereafter ...
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bioRxiv - Microbiology 2021Quote: ... Staining was visualized using biotinylated polyclonal rabbit anti-mouse Ig (Dako) and goat anti-rabbit Ig (Dako) ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
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bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Systems Biology 2023Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Neuroscience 2022Quote: ... the slides were exposed to a mouse-specific biotinylated secondary antibody (GV82111-2, Dako) for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Signal amplification was performed by incubating with biotinylated anti-mouse secondary antibody (E0354, Dako) at 1:400 dilution for 30 minutes at RT ...
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bioRxiv - Physiology 2023Quote: ... we used an amplification step consisting of anti-mouse biotinylated (DAKO, cat no. E0413) or anti-rabbit biotinylated (NOVEX ...
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bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Cell Biology 2021Quote: Constructs encoding the 6His-SUMO tagged proteins were transformed into chemically competent BL21(DE3) Escherichia coli (Agilent). A fresh bacteria colony was inoculated to 10 ml LB broth containing 200 μg/ml Ampicillin and 50 μg/ml Chloramphenicol and grown at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... TAP-tagged proteins were detected using rabbit antiperoxidase antibody linked to peroxidase (PAP, Dako; 1:10000 dilution). Tubulin was detected using mouse-anti-α-tubulin antibody (Sigma-Aldrich T5168 ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Pathology 2019Quote: ... the sections were incubated with the biotinylated secondary antibody (goat anti-rabbit and goat anti-mouse, Dako) for 60 minutes ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Immunology 2021Quote: Biotinylated proteins were isolated from the organ homogenates using a Bravo AssayMap platform and AssayMap streptavidin cartridges (Agilent). The cartridges were equilibrated with ammonium bicarbonate (50mM ...
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bioRxiv - Immunology 2023Quote: ... Antigens were tetramerized by incubation at a >4:1 ratio of biotinylated protein with streptavidin-PE (Agilent; PJRS25) or streptavidin-APC (Agilent ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Immunology 2022Quote: ... The tetrameric detection reagents were generated immediately prior to use by combining biotinylated FcɣR proteins (Ackerman Laboratory) with a 1/4th molar ratio of Streptavidin-Allophycocyanin (ProZyme) and mixing by inversion for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... For PrP deposition anti-PrP monoclonal antibodies ICSM3525,26 were used in conjunction with biotinylated polyclonal rabbit anti-mouse immunoglobulin secondary antibodies (Dako; Agilent) and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...