Labshake search
Citations for Agilent :
1 - 50 of 784 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
-
bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
-
bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
-
bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
-
bioRxiv - Plant Biology 2019Quote: ... β-caryophyllene and 20 mg indole (Erb et al., 2015) was added into separate 2.0 ml amber glass vial (Agilent Technologies) with 1 mg of glass wool (Fig ...
-
bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
-
bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
-
bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
-
bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
-
bioRxiv - Bioengineering 2021Quote: ... and MBP-tagged (for immunoblotting) NC1 domains of type IV Collagen α1-6 (V) were expressed in BL21-CodonPlus-RP (Agilent Technologies, Santa Clara, CA) transformed with pET-28a (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
-
bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
-
bioRxiv - Biophysics 2021Quote: ... pH 5.9) and buffer B (75 % (v/v) buffer A with 25 % (v/v) Methanol) (Agilent 1200). A different concentration gradient of c-di-AMP and AMP were used to prepare a standard curve for c-di-AMP and AMP ...
-
bioRxiv - Microbiology 2021Quote: ... 0.001% (v/v) of StrataClean resin (Agilent) was added in order to bind protein ...
-
bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were incubated with 1:200 (v/v) mouse monoclonal anti-AX AO3.2 [16] and 1:2000 (v/v) anti-mouse IgG-HRP (Dako Cytomation) diluted in 1%(w/v ...
-
bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies tagged with Horse Radish Peroxidase (HRP; Dako) were incubated for 1 h at room temperature under rotation ...
-
bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
-
bioRxiv - Biochemistry 2023Quote: ... and 0.01% medronic acid (v/v, 5191-4506, Agilent). For the re-equilibration gradient ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 μL of AccuScript Hi-Fi (Agilent Technologies) was added up to a 10 μL volume ...
-
bioRxiv - Microbiology 2022Quote: ... A 300×7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
-
bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
-
bioRxiv - Systems Biology 2023Quote: ... A 300 × 7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
-
bioRxiv - Biophysics 2021Quote: ... Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Stratagene, CA, USA). A construct containing the cDNA encoding the isolated PTP domain preceded by the C-SH2 domain (residues 105-528 ...
-
bioRxiv - Cancer Biology 2023Quote: OXPHOS and glycolysis of leukemia cells were assessed by extracellular flux using a Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, CA) with a Cell Mito Stress Test Kit (103015 ...
-
bioRxiv - Biochemistry 2019Quote: ... hexahistidine-tagged IN was overexpressed in BL-21 CodonPlus RIL cells (Agilent). Cells were lysed in 25 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
-
bioRxiv - Developmental Biology 2020Quote: ... Dual-tagged Slit2 Δ construct was obtained by Quickchange mutagenesis procedure (Agilent), deleting 9 amino acids (SPPMVLPRT ...
-
bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... Samples were then incubated with 5% (v/v) Goat or Donkey Serum (Dako) for 30 min ...
-
bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...
-
bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 5% (v/v, no unit definition available) AffinityScript Multiple Temperature Reverse Transcriptase (Agilent). RNA was incubated at 65°C for 5 min and was then left at RT for 10 min for primer annealing ...
-
bioRxiv - Immunology 2023Quote: ... proteins in the supernatant were precipitated with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... Point mutations were introduced into plasmid-encoded viral cDNAs using QuikChange mutagenesis (Agilent) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: All PKR and viral genes were cloned into the pSG5 expression vector (Stratagene) for transient transfection assay ...
-
bioRxiv - Immunology 2023Quote: ... Viral foci were detected using 1:10,000 dilution of αHSV rabbit antibody (Dako) and 1:50,000 dilution of goat αrabbit HRP conjugated antibody ...
-
bioRxiv - Plant Biology 2021Quote: ... Solvent A and B were supplied with 0.1% (v/v) Infinity Lab deactivator additive (Agilent) to improve peak shapes ...
-
bioRxiv - Plant Biology 2023Quote: ... Solvents A and B were supplied with 0.1% (v/v) Infinity Lab deactivator additive (Agilent) to improve peak shapes ...
-
bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
-
bioRxiv - Biochemistry 2021Quote: ... N-terminally His6-tagged KRAS was expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl-β-D-1-thiogalactopyranoside induction at 18 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... HA-tagged DCX mutant T203R were created using QuikChange Site-Directed Mutagenesis kit (Stratagene). HA-tagged DCX mutant A71S was synthesized commercially (Genewiz ...
-
bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
-
bioRxiv - Microbiology 2022Quote: OPG2-tagged ORF3c plasmids were generated by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies) and confirmed by DNA sequencing (GATC ...
-
bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
-
bioRxiv - Cell Biology 2023Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
-
bioRxiv - Immunology 2024Quote: ... C4b deposition was measured using a biotin-tagged anti-C4c polyclonal Ab (Agilent, Q0369) followed by HRP-streptavidin and TMB One ...
-
bioRxiv - Biochemistry 2024Quote: ... the CBP-tagged Drp1 was purified by affinity chromatography using calmodulin agarose resin (Agilent) that had been pre-equilibrated with CalA Buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.1% (v/v) NP-40] was incubated with 40 μg of rabbit anti-mouse antibody (DakoCytomation) in a vertical wheel for 90 min at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: Adeno-associated viral (AAV) vectors were produced using the AAV Helper-Free System (Agilent Technologies ...