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Citations for Agilent :
1 - 50 of 5631 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Molecular Biology 2022Quote: ... Laser box MLC400 (Agilent) Zyla cMOS camera (Andor) ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Cell Biology 2021Quote: Microscopic observation of Arabidopsis plantlets attacked with pathogens was performed using an inverted spinning disk confocal microscope with a high-resolution camera (Yokogawa CSU-X1 on Nikon Ti-E platform, laser box Agilent MLC400 ...
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bioRxiv - Developmental Biology 2020Quote: ... To generate the Sp ΔBTD-box and the btd ΔBTD-box we used the QuickChange Site-Directed Mutagenesis Kit (Stratagene) to delete the 30 bp encoding for the BTD-box using the following primers:
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Systems Biology 2021Quote: ... and an Agilent 6560 Ion Mobility QTOF MS system (Agilent, Santa Clara, CA). The total lipid extracts (TLE ...
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bioRxiv - Cell Biology 2022Quote: ... A Q-TOF mass spectrometer (6560 Ion Mobility; Agilent, Santa Clara, CA, USA) equipped with electrospray ionisation source (Dual AJS ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CA125 epitope group B (Agilent, M11, 1:100), or anti-CA125 epitope group B (Fitzgerald ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Bioengineering 2023Quote: High-performance liquid chromatography (HPLC) (Shimadzu Co.) with an ion exchange column (Agilent Hi-Plex Column) was employed to detect the metabolites of first part of pathway and the final product ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were separated on a Hi-Plex Na column by high-performance liquid chromatography (HPLC) (Agilent Technologies) by applying a flow rate of 0.7 mL/min with water and methanol as mobile phase ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were analyzed using high-performance liquid chromatography (HPLC) with a Hi-Plex Na column (Agilent Technologies). The separation was achieved by applying a flow rate of 0.7 mL/min ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNAs were obtained and amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... we calibrated the ion mobility dimension linearly using at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Synthetic Biology 2023Quote: ... Compounds eluting from the column were analyzed with a 6560 Ion Mobility QTOF mass spectrometer (Agilent Technologies) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... The E-box mutant was constructed with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using primers 5’-cgcgggttcccaccttgtggccagaagatcttctgggcagaactactcgtttggc-3’ and 5’-gccaaacgagtagttctgcccagaagatcttctggccacaaggtgggaacccgcg-3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... or anti-CA125 epitope group B (Agilent, M11 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were analyzed using the 1290 Infinity II LC system coupled to a 6560 Ion Mobility Q-TOF (Agilent Technologies). Samples were analyzed with a Zorbax Eclipse Plus C18 50 × 4.6 mm column (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Low concentration Tuning Mix standard (G1969-85000) was used to calibrate the mass spectrometry and ion mobility spectrometry and obtained from Agilent Technologies (Santa Clara ...
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bioRxiv - Plant Biology 2024Quote: ... and ion mobility calibration was performed using ESI-L low concentration tune mix bought from Agilent (Santa Clara, CA, USA). The 10 mM sodium formate solution contained 1 M NaOH (250 μL ...
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bioRxiv - Bioengineering 2023Quote: ... Staining of the brains was performed in groups consisting of GFAP (DAKO rabbit 1:500) and tomato lectin (Vector Labs mouse 1:250) ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human tau (Dako, 1:10,000), the phosphorylation specific anti-tau antibody Ser396/Ser404 (PHF1 ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD45 (Dako M0701, 1:100), and human CD20 (Dako M0755 ...
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bioRxiv - Cancer Biology 2023Quote: ... human vimentin (1:4000; M0725, DakoCytomation), breast cancer resistance protein (BCRP ...
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bioRxiv - Biophysics 2019Quote: Ion mobility (ESI-IM-MS) experiments were carried out on an Agilent 6560 IMS-Q-TOF (Agilent Technologies, Santa Clara, USA) operated with helium in the linear drift tube IM ...
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bioRxiv - Systems Biology 2023Quote: ... Analysis was performed with an Agilent 6560 Ion Mobility Q-TOF coupled to an Agilent 1290 Infinity II (Agilent Technologies,Inc.). The samples were separated with an YMC-UltraHTPro C18 column (100 × 2 mm i.d ...
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bioRxiv - Microbiology 2021Quote: ... The 5-AIQC-tagged samples (1 μL) were individually injected on an UPLC column (Agilent ZORBAX RRHD Eclipse XDB C18 column ...
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bioRxiv - Plant Biology 2023Quote: ... were transferred to a glass insert and analysed with a 1290 Infinity II coupled to a 6560 Ion Mobility Q-TOF (Agilent Technologies, Inc.) as previously published (Drapal et al. ...
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bioRxiv - Biochemistry 2023Quote: ... external mass and ion mobility calibration was achieved (via ESI source) using ESI-Low Concentration Tuning Mix (Agilent Technologies, Santa Clara, USA) and a linear calibration model ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Immunology 2020Quote: ... anti-human CD3 (Dako A0452; 1:300); anti-human periaxin (PXN ...
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bioRxiv - Cancer Biology 2021Quote: ... and human CD20 (Dako M0755, 1:1000) on the Leica Bond RX automated staining as previously described for the CAV1 IHC method ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-human Tau (1:500, Dako). After incubation with the primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... rabbit anti-human C3d (1:200; Dako), and rabbit anti-human C5b-9 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 unit of high-fidelity polymerase (Stratagene). A C-terminal Cys residue for 5-IAF labeling was introduced by site-directed mutagenesis (QuikChange ...
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bioRxiv - Systems Biology 2019Quote: ... solution before being analysed on an Infinity II UHPLC coupled to an Agilent 6560 Ion Mobility Q-ToF LC-MS (Agilent, Santa Clara, USA), using a 20 min reverse-phase chromatographic separation with a C18 Acquity column (1.7 μm ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... the final nucleotide of the TATA box of pCMV-Gluc2 was changed to a T by using site-directed mutagenesis (QuikChange lightning; Agilent) to facilitate in vitro transcription (54) ...